背景与目的日间手术的种类和数量在不断扩大,部分经过选择的肺癌患者进行日间手术,临床效果如何?基于加速康复外科(enhanced recovery after surgery, ERAS)理念和外科微创技术,探索肺癌患者日间手术的操作流程及其临床应用效果。方法选...背景与目的日间手术的种类和数量在不断扩大,部分经过选择的肺癌患者进行日间手术,临床效果如何?基于加速康复外科(enhanced recovery after surgery, ERAS)理念和外科微创技术,探索肺癌患者日间手术的操作流程及其临床应用效果。方法选取2019年6月-2019年11月四川大学华西胸外科单个医疗组连续收治行肺手术患者150例,最终纳入研究患者48例,其中住院手术(inpatient surgery group, ISG)患者28例和日间手术患者(day surgery group,DSG)20例。分析两组患者平均住院日、住院费用及并发症等。结果平均住院日在日间手术组(1 d)显著低于住院手术组(7.7±2.8)d(P=0.000);平均住院费用在日间手术组(38,297.3±3408.7)$显著低于住院手术组(47,831.1±7376.1)$(P=0.000)。术后总体并发症发生率在日间手术组(5.0%)与住院手术组(3.6%)无统计学差异(P=0.812)。术后总体不良反应发生率日间手术组(10.0%)与住院手术组(17.9%)无统计学差异(P=0.729)。结论经过选择的肺癌患者行日间手术是可行的且能够加速康复。展开更多
Objective:Patient-derived xenograft(PDX)models have shown great promise in preclinical and translational applications,but their consistency with primary tumors in phenotypic,genetic,and pharmacodynamic heterogeneity h...Objective:Patient-derived xenograft(PDX)models have shown great promise in preclinical and translational applications,but their consistency with primary tumors in phenotypic,genetic,and pharmacodynamic heterogeneity has not been well-studied.This study aimed to establish a PDX repository for non-small cell lung cancer(NSCLC)and to further elucidate whether it could preserve the heterogeneity within and between tumors in patients.Methods:A total of 75 surgically resected NSCLC specimens were implanted into immunodeficient NOD/SCID mice.Based on the successful establishment of the NSCLC PDX model,we compared the expressions of vimentin,Ki67,EGFR,and PD-L1 proteins between cancer tissues and PDX models using hematoxylin and eosin staining and immunohistochemical staining.In addition,we detected whole gene expression profiling between primary tumors and PDX generations.We also performed whole exome sequencing(WES)analysis in 17 first generation xenografts to further assess whether PDXs retained the patient heterogeneities.Finally,paclitaxel,cisplatin,doxorubicin,atezolizumab,afatininb,and AZD4547 were used to evaluate the responses of PDX models to the standard-of-care agents.Results:A large collection of serially transplantable PDX models for NSCLC were successfully developed.The histology and pathological immunohistochemistry of PDX xenografts were consistent with the patients’tumor samples.WES and RNA-seq further confirmed that PDX accurately replicated the molecular heterogeneities of primary tumors.Similar to clinical patients,PDX models responded differentially to the standard-of-care treatment,including chemo-,targeted-and immuno-therapeutics.Conclusions:Our established PDX models of NSCLC faithfully reproduced the molecular,histopathological,and therapeutic characteristics,as well as the corresponding tumor heterogeneities,which provides a clinically relevant platform for drug screening,biomarker discovery,and translational research.展开更多
We process the standard 30 s, static GPS data and the 1 s, high-rate GPS （HRGPS） data provided by the Crustal Movement Observation Network of China with GAMIT/GLOBK software package, and obtain the co- seismic displ...We process the standard 30 s, static GPS data and the 1 s, high-rate GPS （HRGPS） data provided by the Crustal Movement Observation Network of China with GAMIT/GLOBK software package, and obtain the co- seismic displacements of near field and far field, and the epoch-by-epoch time series of HRGPS during Lushan earthquake. GPS data from about 20 sites in Sichuan province, which located between 40 and 450 km from the epicenter, are analyzed so as to study the characteristics of the static displacements and the dynamic crustal defor- mations, with periods ranging from several minutes to over a month. The result shows that： the static displacements caused by Lushan earthquake are limited to several centi- meters; the nearest station SCTQ at 43 km from the epi- center has the largest static displacement of about 2 cm, while the other stations generally have insignificant dis- placements of less than 5 mm. the stations in the east ofSichuan-Yunnan region shifts 5-10 mm toward the southwest, and the stations in the middle-west of Sichuan Basin moves indistinctively 1-2 mm toward the northwest; station SCTQ has the largest kinematic displacement of about 4 and 3 cm peak-to-peak on the north and east component, respectively, and is much greater than the static permanent displacement; for the stations located at a distance greater than 150 km from the epicenter, the kinematic motions are generally insignificant; exception- ally, station SCNC and station SCSN in central Sichuan Basin have significant kinematic motions although they are more than 200 km away from the epicenter.展开更多
Background:The incidence rate of lung cancer in women has significantly increased over the past decade,and previous evidence has indicated a significant relationship between the elevated levels of sex hormones and the...Background:The incidence rate of lung cancer in women has significantly increased over the past decade,and previous evidence has indicated a significant relationship between the elevated levels of sex hormones and the risk of lung cancer.Therefore,we hypothesized that female hormone-related cancer(FHRC)patients,including breast,endometrial,cervical,and ovarian cancer patients,may experience a higher risk of developing subsequent lung cancer.This meta-analysis aimed to identify the risk of lung cancer among FHRC patients compared to the general population.Methods:The PubMed,Web of Science,EMBASE,Cochrane Library,and CNKI databases were searched up to May 11,2022.Standardized incidence ratios(SIRs)with 95%confidence intervals(CIs)were used to identify the risk of subsequent lung cancer after FHRC.Subgroup analyses based on the follow-up time and tumor type were also conducted.Results:A total of 58 retrospective cohort studies involving 4,360,723 FHRC participants were included.The pooled results demonstrated that FHRC patients had a significantly increased risk of developing subsequent primary lung cancer(SIR=1.61,95%CI:1.48-1.76,P<0.001).Subgroup analysis revealed an obvious trend of increasing lung cancer risk over time(SIRs for<5 years,≥5 years,≥10 years,≥20 years,and≥30 years after FHRC:1.32,1.59,1.57,1.68,and 1.95,respectively).In addition,subgroup analysis stratified by tumor type indicated an increased risk of developing subsequent lung cancer after breast(SIR=1.25,P<0.001),endometrial(SIR=1.40,P=0.019),cervical(SIR=2.56,P<0.001),and ovarian cancer(SIR=1.50,P=0.010).Conclusion:FHRC patients are more likely to develop lung cancer than the general population.Furthermore,the increased risk of subsequent primary lung cancer is more obvious with a longer survival time and is observed in all types of hormone-related cancer.Registration:International Platform of Registered Systematic Review and Meta-analysis Protocols:No.INPLASY202270044;https://inplasy.com/.展开更多
文摘背景与目的日间手术的种类和数量在不断扩大,部分经过选择的肺癌患者进行日间手术,临床效果如何?基于加速康复外科(enhanced recovery after surgery, ERAS)理念和外科微创技术,探索肺癌患者日间手术的操作流程及其临床应用效果。方法选取2019年6月-2019年11月四川大学华西胸外科单个医疗组连续收治行肺手术患者150例,最终纳入研究患者48例,其中住院手术(inpatient surgery group, ISG)患者28例和日间手术患者(day surgery group,DSG)20例。分析两组患者平均住院日、住院费用及并发症等。结果平均住院日在日间手术组(1 d)显著低于住院手术组(7.7±2.8)d(P=0.000);平均住院费用在日间手术组(38,297.3±3408.7)$显著低于住院手术组(47,831.1±7376.1)$(P=0.000)。术后总体并发症发生率在日间手术组(5.0%)与住院手术组(3.6%)无统计学差异(P=0.812)。术后总体不良反应发生率日间手术组(10.0%)与住院手术组(17.9%)无统计学差异(P=0.729)。结论经过选择的肺癌患者行日间手术是可行的且能够加速康复。
基金supported by the National Natural Science Foundation of China(Grant Nos.81101143,81572617,and 81630101)the Sichuan Province Science and Technology Support Program(Grant Nos.2019JDRC0019 and 2018SZ0009)+2 种基金1.3.5 project for disciplines of excellence,West China Hospital,Sichuan University(Grant No.ZYJC18026)The Science and Technology Project of the Health Planning Committee of Sichuan(Grant No.19PJ242)Chengdu science and technology Support Program(Grant No.2019-YFYF-00090-SN)。
文摘Objective:Patient-derived xenograft(PDX)models have shown great promise in preclinical and translational applications,but their consistency with primary tumors in phenotypic,genetic,and pharmacodynamic heterogeneity has not been well-studied.This study aimed to establish a PDX repository for non-small cell lung cancer(NSCLC)and to further elucidate whether it could preserve the heterogeneity within and between tumors in patients.Methods:A total of 75 surgically resected NSCLC specimens were implanted into immunodeficient NOD/SCID mice.Based on the successful establishment of the NSCLC PDX model,we compared the expressions of vimentin,Ki67,EGFR,and PD-L1 proteins between cancer tissues and PDX models using hematoxylin and eosin staining and immunohistochemical staining.In addition,we detected whole gene expression profiling between primary tumors and PDX generations.We also performed whole exome sequencing(WES)analysis in 17 first generation xenografts to further assess whether PDXs retained the patient heterogeneities.Finally,paclitaxel,cisplatin,doxorubicin,atezolizumab,afatininb,and AZD4547 were used to evaluate the responses of PDX models to the standard-of-care agents.Results:A large collection of serially transplantable PDX models for NSCLC were successfully developed.The histology and pathological immunohistochemistry of PDX xenografts were consistent with the patients’tumor samples.WES and RNA-seq further confirmed that PDX accurately replicated the molecular heterogeneities of primary tumors.Similar to clinical patients,PDX models responded differentially to the standard-of-care treatment,including chemo-,targeted-and immuno-therapeutics.Conclusions:Our established PDX models of NSCLC faithfully reproduced the molecular,histopathological,and therapeutic characteristics,as well as the corresponding tumor heterogeneities,which provides a clinically relevant platform for drug screening,biomarker discovery,and translational research.
基金supported by the National Natural Science Foundation of China(Granted Nos.41174029 and 41204028)the Polar Strategic Research Foundation of China(Granted No.20110205)+1 种基金the Open Research Fund of Key Laboratory for Polar Science of State Oceanic Administration(Granted No.KP201201)the Science and Technology Project of National Administration of Surveying,Mapping,and Geoinformation(Granted name Polar Geomatics Technology Test)
文摘We process the standard 30 s, static GPS data and the 1 s, high-rate GPS （HRGPS） data provided by the Crustal Movement Observation Network of China with GAMIT/GLOBK software package, and obtain the co- seismic displacements of near field and far field, and the epoch-by-epoch time series of HRGPS during Lushan earthquake. GPS data from about 20 sites in Sichuan province, which located between 40 and 450 km from the epicenter, are analyzed so as to study the characteristics of the static displacements and the dynamic crustal defor- mations, with periods ranging from several minutes to over a month. The result shows that： the static displacements caused by Lushan earthquake are limited to several centi- meters; the nearest station SCTQ at 43 km from the epi- center has the largest static displacement of about 2 cm, while the other stations generally have insignificant dis- placements of less than 5 mm. the stations in the east ofSichuan-Yunnan region shifts 5-10 mm toward the southwest, and the stations in the middle-west of Sichuan Basin moves indistinctively 1-2 mm toward the northwest; station SCTQ has the largest kinematic displacement of about 4 and 3 cm peak-to-peak on the north and east component, respectively, and is much greater than the static permanent displacement; for the stations located at a distance greater than 150 km from the epicenter, the kinematic motions are generally insignificant; exception- ally, station SCNC and station SCSN in central Sichuan Basin have significant kinematic motions although they are more than 200 km away from the epicenter.
基金funded by the Sichuan Science and Technology Program(No.2020YFS0252)the Natural Science Foundation of Sichuan Province(No.2022NSFSC1464).
文摘Background:The incidence rate of lung cancer in women has significantly increased over the past decade,and previous evidence has indicated a significant relationship between the elevated levels of sex hormones and the risk of lung cancer.Therefore,we hypothesized that female hormone-related cancer(FHRC)patients,including breast,endometrial,cervical,and ovarian cancer patients,may experience a higher risk of developing subsequent lung cancer.This meta-analysis aimed to identify the risk of lung cancer among FHRC patients compared to the general population.Methods:The PubMed,Web of Science,EMBASE,Cochrane Library,and CNKI databases were searched up to May 11,2022.Standardized incidence ratios(SIRs)with 95%confidence intervals(CIs)were used to identify the risk of subsequent lung cancer after FHRC.Subgroup analyses based on the follow-up time and tumor type were also conducted.Results:A total of 58 retrospective cohort studies involving 4,360,723 FHRC participants were included.The pooled results demonstrated that FHRC patients had a significantly increased risk of developing subsequent primary lung cancer(SIR=1.61,95%CI:1.48-1.76,P<0.001).Subgroup analysis revealed an obvious trend of increasing lung cancer risk over time(SIRs for<5 years,≥5 years,≥10 years,≥20 years,and≥30 years after FHRC:1.32,1.59,1.57,1.68,and 1.95,respectively).In addition,subgroup analysis stratified by tumor type indicated an increased risk of developing subsequent lung cancer after breast(SIR=1.25,P<0.001),endometrial(SIR=1.40,P=0.019),cervical(SIR=2.56,P<0.001),and ovarian cancer(SIR=1.50,P=0.010).Conclusion:FHRC patients are more likely to develop lung cancer than the general population.Furthermore,the increased risk of subsequent primary lung cancer is more obvious with a longer survival time and is observed in all types of hormone-related cancer.Registration:International Platform of Registered Systematic Review and Meta-analysis Protocols:No.INPLASY202270044;https://inplasy.com/.
