Emodin[1,3,8-Trihydroxy-6-methylanthraquinone]has been reported to exhibit vascular anti-inflammatory properties.However,the relevant anti-inflammatory mechanisms are not well understood.The present study was design t...Emodin[1,3,8-Trihydroxy-6-methylanthraquinone]has been reported to exhibit vascular anti-inflammatory properties.However,the relevant anti-inflammatory mechanisms are not well understood.The present study was design to explore the molecular target(s)of emodin in modifying lipopolysaccharide(LPS)-associated signal transduction pathway in human umbilical vein endothelial cells(HUVECs).Cultured HUVECs were pre-incubated with 1 to 50μg/ml emodin for 30 min,LPS-induced proinflammatory cytokines(IL-1,IL-6)and chemokines(IL-8,MCP-1)expression were inhibited dose-dependently,which agreed well with the NF-B activation and IB degradation detected by immunocytochemistry and western blotting,respectively.展开更多
基金supported by National Natural Science Foundation of China,30700151Youth Investigator Fund from UESTC,Y02018023601062
文摘Emodin[1,3,8-Trihydroxy-6-methylanthraquinone]has been reported to exhibit vascular anti-inflammatory properties.However,the relevant anti-inflammatory mechanisms are not well understood.The present study was design to explore the molecular target(s)of emodin in modifying lipopolysaccharide(LPS)-associated signal transduction pathway in human umbilical vein endothelial cells(HUVECs).Cultured HUVECs were pre-incubated with 1 to 50μg/ml emodin for 30 min,LPS-induced proinflammatory cytokines(IL-1,IL-6)and chemokines(IL-8,MCP-1)expression were inhibited dose-dependently,which agreed well with the NF-B activation and IB degradation detected by immunocytochemistry and western blotting,respectively.
