Astrocyte maturation is crucial for brain function,yet the mechanisms regulating this process remain poorly understood.In this study,we identify the bHLH transcription factors Olig1 and Olig2 as essential coordinators...Astrocyte maturation is crucial for brain function,yet the mechanisms regulating this process remain poorly understood.In this study,we identify the bHLH transcription factors Olig1 and Olig2 as essential coordinators of cortical astrocyte maturation.We demonstrate that Olig1 and Olig2 work synergistically to regulate cortical astrocyte maturation by modulating Bmp7 expression.Genetic ablation of both Olig1 and Olig2 results in defective astrocyte morphology,including reduced process complexity and an immature gene expression profile.Single-cell RNA sequencing reveals a shift towards a less mature astrocyte state,marked by elevated levels of HOPX and GFAP,resembling human astrocytes.Mechanistically,Olig1 and Olig2 bind directly to the Bmp7 enhancer,repressing its expression to promote astrocyte maturation.Overexpression of Bmp7 in vivo replicates the astrocyte defects seen in Olig1/2 double mutants,confirming the critical role of BMP7 signaling in this process.These findings provide insights into the transcriptional and signaling pathways regulating astrocyte development and highlight Olig1 and Olig2 as key regulators of cortical astrocyte maturation,with potential implications for understanding glial dysfunction in neurological diseases.展开更多
Traditional metal sulfides used as anodes for sodium-ion batteries are hindered by sluggish kinetics,which limits their rate performance.Previous attempts to address this issue focused on nanostructured configurations...Traditional metal sulfides used as anodes for sodium-ion batteries are hindered by sluggish kinetics,which limits their rate performance.Previous attempts to address this issue focused on nanostructured configurations with conductive frameworks.However,these nanomaterials often suffer from low packing density and the tendency for nanoparticles to agglomerate,posing significant challenges for practical applications.To overcome these limitations,this study presents a novel bimetal superionic anode material Cu_(3.21)Bi_(4.79)S_(9),which effectively resolves the conflict between sluggish kinetics and micrometer-scale particle size.By leveraging the vacancies created by free Cu and Bi atoms,this material forms rapid migration channels during sodium insertion and extraction,significantly reducing the migration barriers for sodium ions.The development of micrometer-scale Cu_(3.21)Bi_(4.79)S_(9)enables ultrafast chargingdischarging capabilities,achieving a reversible capacity of 325.5 mAh g^(-1)after 4000 cycles at a high rate of 45 C(15 A g^(-1)).This work marks a significant advancement in the field by offering a solution to the inherent trade-off between high capacity and rate performance in coarse-grained materials,reducing the need for reliance on nanostructured configurations for next-generation high-capacity anode materials.展开更多
Human cortical radial glial cells are primary neural stem cells that give rise to cortical glutaminergic projection pyramidal neurons, glial cells (oligodendrocytes and astrocytes) and olfactory bulb GABAergic interne...Human cortical radial glial cells are primary neural stem cells that give rise to cortical glutaminergic projection pyramidal neurons, glial cells (oligodendrocytes and astrocytes) and olfactory bulb GABAergic interneurons. One of prominent features of the human cortex is enriched with glial cells, but there are major gaps in understanding how these glial cells are generated. Herein, by integrating analysis of published human cortical single-cell RNA-Seq datasets with our immunohistochemistical analyses, we show that around gestational week 18, EGFR-expressing human cortical truncated radial glial cells (tRGs) give rise to basal multipotent intermediate progenitors (bMIPCs) that express EGFR, ASCL1, OLIG2 and OLIG1. These bMIPCs undergo several rounds of mitosis and generate cortical oligodendrocytes, astrocytes and olfactory bulb interneurons. We also characterized molecular features of the cortical tRG. Integration of our findings suggests a general picture of the lineage progression of cortical radial glial cells, a fundamental process of the developing human cerebral cortex.展开更多
Mouse cortical radial glial cells(RGCs)are primary neural stem cells that give rise to cortical oligodendrocytes,astrocytes,and olfactory bulb(OB)GABAergic interneurons in late embryogenesis.There are fundamental gaps...Mouse cortical radial glial cells(RGCs)are primary neural stem cells that give rise to cortical oligodendrocytes,astrocytes,and olfactory bulb(OB)GABAergic interneurons in late embryogenesis.There are fundamental gaps in understanding how these diverse cell subtypes are generated.Here,by combining single-cell RNA-Seq with intersectional lineage analyses,we show that beginning at around E16.5,neocortical RGCs start to generate ASCL1^(+)EGFR^(+)apical multipotent intermediate progenitors(MIPCs),which then differentiate into basal MIPCs that express ASCL1,EGFR,OLIG2,and MKI67.These basal MIPCs undergo several rounds of divisions to generate most of the cortical oligodendrocytes and astrocytes and a subpopulation of OB interneurons.Finally,single-cell ATAC-Seq supported our model for the genetic logic underlying the specification and differentiation of cortical glial cells and OB interneurons.Taken together,this work reveals the process of cortical radial glial cell lineage progression and the developmental origins of cortical astrocytes and oligodendrocytes.展开更多
Locomotion stability is essential for controlling quadruped robots and adapting them to unstructured terrain.We propose a control strategy with center-of-mass(CoM)dynamic planning for the stable locomotion of these ro...Locomotion stability is essential for controlling quadruped robots and adapting them to unstructured terrain.We propose a control strategy with center-of-mass(CoM)dynamic planning for the stable locomotion of these robots.The motion trajectories of the swing legs are synchronized with the CoM of the robot.To implement the synchronous control scheme,we adjusted the swing legs to form a support triangle.The strategy is applicable to both static walk gait and dynamic trot gait.In the motion control processes of the robot legs,the distribution of the ground reaction forces is optimized to minimize joint torque and locomotion energy consumption.We also used an improved joint-torque controller with varied controller coefficients in the stance and swing phases.The simulation and experimental results demonstrate that the robot can complete omnidirectional locomotion in both walk and trot gaits.At a given locomotion speed,the stability margins for the robot during walking and trotting were 27.25%and 37.25%higher,respectively,than in the scheme without CoM planning.The control strategy with energy consumption optimization(ECO)reduced the energy consumption of the robot in walk and trot gaits by 11.25%and 13.83%,respectively,from those of the control scheme without ECO.展开更多
This paper discusses H-infinity state feedback control for a networked control system with time-varying delays. Based on the flee-weighing matrix method, a dehy-dependent stability criterion satisfying a prescribed H-...This paper discusses H-infinity state feedback control for a networked control system with time-varying delays. Based on the flee-weighing matrix method, a dehy-dependent stability criterion satisfying a prescribed H-infinity norm bound is presented for an NCS with unknown, time-varying and bounded delays. And then, the criterion is transformed into sufficient conditions based on linear matrix inequalities for H-infinity control. The conditions thus obtained are also used to design an H-infinity state feedback controller. This design method is further extended to solve the design problem of robust H-infinity state feedback control. A numerical example demonstrates the validity of the method.展开更多
This paper investigates the remote tracking control problem of Network-based Agents with communication delays existing in both forward and feedback communication channels.A networked predictive tracking controller is ...This paper investigates the remote tracking control problem of Network-based Agents with communication delays existing in both forward and feedback communication channels.A networked predictive tracking controller is proposed to compensate the negative effects caused by bilateral time-delays in a wireless network.Furthermore,the problem of consecutive data loss in the feedback channel is solved using aforementioned controller,where lateral movement perturbations are introduced.Simulations and experiments are provided for several cases,which verify the realizability and effectiveness of the proposed controller.展开更多
Fractures are of great significance to tight oil and gas development.Fracture identification using conventional well logs is a feasible way to locate the underground fractures in tight sandstones.However,there are thr...Fractures are of great significance to tight oil and gas development.Fracture identification using conventional well logs is a feasible way to locate the underground fractures in tight sandstones.However,there are three problems affecting its interpretation accuracy and practical application,namely weak well log responses of fractures,a lack of specific logs for fracture prediction,and relative change omission in log responses.To overcome these problems and improve fracture identification accuracy,a fracture indicating parameter(FIP)method composed of a comprehensive index method(CIM)and a comprehensive fractal method(CFM)is introduced.The CIM tries to handle the first problem by amplifying log responses of fractures.The CFM addresses the third one using fractal dimensions.The flexible weight parameters corresponding to logs in the CIM and CFM make the interpretation possible for wells lacking specific logs.The reconstructed logs in the CIM and CFM try to solve the second problem.It is noted that the FIP method can calculate the probability of fracture development at a certain depth,but cannot show the fracture development degree of a new well compared with other wells.In this study,a formation fracture intensity(FFI)method is also introduced to further evaluate fracture development combined with production data.To test the validity of the FIP and FFI methods,fracture identification experiments are implemented in a tight reservoir in the Ordos Basin.The results are consistent with the data of rock core observation and production,indicating the proposed methods are effective for fracture identification and evaluation.展开更多
Chronic occlusion of large intracranial arteries is the main cause of ischemic stroke in China.Patients with symptomatic intracranial artery occlusion and hemodynamic impairment are at high risk of recurrent stroke.Ch...Chronic occlusion of large intracranial arteries is the main cause of ischemic stroke in China.Patients with symptomatic intracranial artery occlusion and hemodynamic impairment are at high risk of recurrent stroke.Chronic occlusion of the intracranial segment of the internal carotid artery is a common type of intracranial artery occlusion.Medical management is regarded as the standard treatment for this disease.With the development of endovascular treatment,some patients with chronic cerebral artery occlusion have achieved satisfactory results with endovascular therapy.We reported a patient with symptomatic chronic occlusion of the ophthalmic segment of the internal carotid artery.Simple balloon angioplasty was performed,and the occluded ophthalmic segment of the internal carotid artery was successfully recanalized without perioperative complications.At 4 months followup,the internal carotid artery remained patent and perfusion of the right cerebral hemisphere improved dramatically.In addition,we briefly reviewed the relevant literature.展开更多
Molybdenum disulfide(MoS_(2))has garnered significant attention in the field of catalysis due to the high density of active sites in its unique two-dimensional(2D)structure,which could be developed into numerous high-...Molybdenum disulfide(MoS_(2))has garnered significant attention in the field of catalysis due to the high density of active sites in its unique two-dimensional(2D)structure,which could be developed into numerous high-performance catalysts.The synthesis of ultra-small MoS2 particles(<10 nm)is highly desired in various experimental studies.The ultra-small structure could often lead to a distinct S-Mo coordination state and nonstoichiometric composition in MoSx,minimizing in-plane active sites of the 2D structure and making it probable to regulate the atomic and electronic structure of its intrinsic active sites on a large extent,especially in MoSx clusters.This article summarizes the recent progress of catalysis over ultra-small undoped MoS_(2) particles for renewable fuel production.Through a systematic review of their synthesis,structural,and spectral characteristics,as well as the relationship between their catalytic performance and inherent defects,we aim to provide insights into catalysis over this matrix that may potentially enable advancement in the development of high-performance MoS_(2)-based catalysts for sustainable energy generation in the future.展开更多
OBJECTIVE: Apply spectral clustering to analyze the patterns of symptoms in patients with chronic gastritis(CG).METHODS: Based on 919 CG subjects, we applied mutual information feature selection to choose the positive...OBJECTIVE: Apply spectral clustering to analyze the patterns of symptoms in patients with chronic gastritis(CG).METHODS: Based on 919 CG subjects, we applied mutual information feature selection to choose the positively correlated symptoms with each pattern.Then, we used the Shi and Malik spectral clustering algorithm to select the top 20 correlated symptoms.RESULTS: We ascertained the results of six patterns.There were three categories for the pattern of accumulation of damp heat in the spleen-stomach(0.00332). There were six categories for the pattern of dampness obstructing the spleen-stomach(0.02466). There were two categories for the pattern of spleen-stomach Qi deficiency(0.013 89).There were three categories for the pattern of spleen-stomach deficiency cold(0.009 15). There were five categories for the pattern of liver-Qistagnation(0.01910).There were four categories for the pattern of stagnant heat in the liver-stomach(0.00585).CONCLUSION: Most of the spectral clustering results of the symptoms of CG patterns were in accordance with clinical experience and Traditional Chinese Medicine theory. Most categories suggested the nature and/or location of the disease.展开更多
Medium spiny neurons(MSNs)in the striatum,which can be divided into D1 and D2 MSNs,originate from the lateral ganglionic eminence(LGE).Previously,we reported that Six3 is a downstream target of Sp8/Sp9 in the transcri...Medium spiny neurons(MSNs)in the striatum,which can be divided into D1 and D2 MSNs,originate from the lateral ganglionic eminence(LGE).Previously,we reported that Six3 is a downstream target of Sp8/Sp9 in the transcriptional regulatory cascade of D2 MSN development and that conditionally knocking out Six3 leads to a severe loss of D2 MSNs.Here,we showed that Six3 mainly functions in D2 MSN precursor cells and gradually loses its function as D2 MSNs mature.Conditional deletion of Six3 had little effect on cell proliferation but blocked the differentiation of D2 MSN precursor cells.In addition,conditional overexpression of Six3 promoted the differentiation of precursor cells in the LGE.We measured an increase of apoptosis in the postnatal striatum of conditional Six3-knockout mice.This suggests that,in the absence of Six3,abnormally differentiated D2 MSNs are eliminated by programmed cell death.These results further identify Six3 as an important regulatory element during D2 MSN differentiation.展开更多
A theoretical model of a humidifier of proton exchange membrane （PEM） fuel cell systems is developed and analyzed first in this paper. The model shows that there exists a strong nonlinearity in the system. Then, the...A theoretical model of a humidifier of proton exchange membrane （PEM） fuel cell systems is developed and analyzed first in this paper. The model shows that there exists a strong nonlinearity in the system. Then, the system is identified using a wavelet networks method. To avoid the curse-of-dimensionality problem, a class of wavelet networks proposed by Billings is employed. The experimental data acquired from the test bench are used for identification. The one-step-ahead predictions and the five-step-ahead predictions are compared with the real measurements, respectively. It shows that the identified model can effectively describe the real system.展开更多
Dear Editor,This letter presents a novel data-driven trajectory planning and control scheme for the unmanned ground vehicles(UGVs).A recent work[1]has demonstrated the effectiveness of approximating the optimal state ...Dear Editor,This letter presents a novel data-driven trajectory planning and control scheme for the unmanned ground vehicles(UGVs).A recent work[1]has demonstrated the effectiveness of approximating the optimal state feedback for a nonlinear unmanned system via deep neural network(DNN).展开更多
[Objectives] This study was conducted to illustrate the possible effects of heredity on the growth,reproduction and immune traits of Enshi Black pig.[Methods]The main growth,reproduction and immune traits of three pop...[Objectives] This study was conducted to illustrate the possible effects of heredity on the growth,reproduction and immune traits of Enshi Black pig.[Methods]The main growth,reproduction and immune traits of three populations( Goutou type,Shizitou type and an intermediate type between them) of Enshi Black pig were surveyed,and the correlations among these traits were analyzed.[Results]The health score of pregnant sows,average litter size and weight gain of piglets of intermediate type were all very significantly higher than those of Goutou and Shizitou types( P < 0.01).The percentage of lymphocytes of intermediate type was significantly higher than that of Goutou and Shizitou types( P < 0.05).The number of white blood cells( WBCs) and the number of neutrophils of intermediate type very were significantly higher than those of the other two types( P < 0.01).The percentages of CD3 + CD4 + double positive T cells( helper/inducer T lymphocytes) and CD3 + CD8 + double positive T cells( suppressor/cytotoxic T lymphocytes) and the CD4 +/CD8 + ratio( the ratio of helper T cells/suppressor T cells) of intermediate type were all significantly higher than those of the other two types.Correlation analysis showed that there was a very significant positive correlation between health score and litter size( P < 0.01,R = 0.336),a significant negative correlation between the percentage of lymphocytes and the number of neutrophils( P < 0.05,R =-0.733),and a significant correlation between the percentage of CD3 + CD8 + T cells and the number of platelets( P < 0.05,R = 0.700).[Conclusions] The intermediate type Enshi Black pig inherits the excellent traits of both Goutou type and Shizitou types,and thus has better growth,reproduction and immune traits than the other two types.展开更多
Background Although stent-assisted coiling has become a standard approach for treating intracranial aneurysms(IAs),there are limited reports on its safety and effectiveness in parent artery less than 2.5 mm in diamete...Background Although stent-assisted coiling has become a standard approach for treating intracranial aneurysms(IAs),there are limited reports on its safety and effectiveness in parent artery less than 2.5 mm in diameter.This study evaluates the feasibility,safety,and short-term outcomes of using Neuroform Atlas stent-assisted coiling for IAs with small parent vessels.Methods This study reviewed and analyzed the clinical data of 50 IAs in 50 patients with a parent artery diameter of≤2.5 mm,treated with Neuroform Atlas stent-assisted coiling at a single center between November 2020 and April 2024.Immediate postoperative angiographic outcomes were assessed using the modified Raymond-Roy classification.Follow-up imaging included computed tomographic angiography(CTA),magnetic resonance angiography(MRA),and digital subtraction angiography(DSA).Clinical outcomes were evaluated using the modified Rankin Scale(mRS).Results The procedures achieved a 100%success rate.Immediately after treatment,24 cases were classified as Raymond-Roy grade I,11 as grade II,and 15 as grade III.Follow-up angiography in 28 cases revealed three instances of aneurysm recurrence,with a secondary procedure performed in one case.One patient reported poor neurological status,and two cases experienced procedure-related adverse events during telephone or clinical follow-up.Conclusions the Atlas stent demonstrated favorable outcomes in the treatment of aneurysms in small parent arteries(<2.5 mm),with a low complication rate.The timely postoperative use of tirofiban may further reduce the risk of ischemic complications.展开更多
A field investigation of arboviruses was conducted in Dejiang, Guizhou Province in the summer of 2016. A total of 8,795 mosquitoes, belonging to four species of three genera, and 1,300 midges were collected. The mosqu...A field investigation of arboviruses was conducted in Dejiang, Guizhou Province in the summer of 2016. A total of 8,795 mosquitoes, belonging to four species of three genera, and 1,300 midges were collected. The mosquito samples were identified on site according to their morphology, and the pooled samples were ground and centrifuged in the laboratory. The supernatant was incubated with mosquito tissue culture cells(C6/36) and mammalian cells(BHK-21) for virus isolation. The results indicated that 40%(3,540/8,795) were Anopheles sinensis, 30%(2,700/8,795) were Culex pipiens quinquefasciatus, and 29%(2,530/8,795)were Armigeres subbalbeatus. Furthermore, a total of eight virus isolates were obtained, and genome sequencing revealed two Zika viruses(ZIKVs) isolated from Culex pipiens quinquefasciatus and Armigeres subbalbeatus, respectively; three Japanese encephalitis viruses(JEVs) isolated from Culex pipiens quinquefasciatus; two Banna viruses(BAVs) isolated from Culex pipiens quinquefasciatus and Anopheles sinensis, respectively; and one densovirus(DNV) isolated from Culex pipiens quinquefasciatus.The ZIKVs isolated from the Culex pipiens quinquefasciatus and Armigeres subbalbeatus mosquitoes represent the first ZIKV isolates in China's Mainland. This discovery presents new challenges for the prevention and control of ZIKV in China, and prompts international cooperation on this global issue.展开更多
The dynamic extracellular matrix(ECM)constantly affects the behaviors of cells.To mimic the dynamics of ECM with controllable stiffness and energy dissipation,this study proposes a strategy in which a small molecule,3...The dynamic extracellular matrix(ECM)constantly affects the behaviors of cells.To mimic the dynamics of ECM with controllable stiffness and energy dissipation,this study proposes a strategy in which a small molecule,3,4-dihydroxybenzaldehyde(DB),was used as fast"dynamic bridges"to construct viscoelastic gelatin methacryloyl(GelMA)-based hydrogels.The storage modulus and loss modulus of hydrogels were independently adjusted by the covalent crosslinking density and by the number of dynamic bonds.The hydrogels exhibited self-healing property,injectability,excellent adhesion and mechanical properties.Moreover,the in vitro results revealed that the viscous dissipation of hydrogels favored the spreading,proliferation,osteogenesis and chondrogenesis of bone marrow mesenchymal stem cells(BMSCs),but suppressed their adipogenesis.RNA-sequencing and immunofluorescence suggested that the viscous dissipation of hydrogels activated Yes-associated protein(YAP)by stabilizing integrinβ1,and further promoted nuclear translocation of smad2/3 andβ-catenin to enhance chondrogenesis and osteogenesis.As a result,the viscoelastic GelMA hydrogels with highest loss modulus showed best effect in cartilage and subchondral bone repair.Taken together,findings from this study reveal an effective strategy to fabricate viscoelastic hydrogels for modulating the interactions between cells and dynamic ECM to promote tissue regeneration.展开更多
The fracture-cave reservoirs controlled by strike-slip faults are the main targets for oil and gas exploration of ultradeep carbonate in the Tarim Basin.It is of great significance to clarify the distribution rules of...The fracture-cave reservoirs controlled by strike-slip faults are the main targets for oil and gas exploration of ultradeep carbonate in the Tarim Basin.It is of great significance to clarify the distribution rules of fractures related to strike-slip faults for guiding the exploration and development of ultra-deep oil and gas.In this study,six groups of strike-slip fault 3D models based on discrete element numerical simulation method have been created to investigate characteristics of fault-related fracture development and distribution law.In addition,we compared the modeling results to the measurement of fractures from the outcrop of a strike-slip fault in the Northern Tarim Basin to verify their validity.The results show that the stress environment is stable in the simple strike-slip section,and fractures intersecting with the strike-slip direction at a small angle are the principal fracture.In the releasing stepover and double-bend sections,the maximum principal stress changes from horizontal to vertical during the formation of pull-apart zones,where the principal fractures intersect the strike-slip direction at a large angle.The maximum principal stress in the restraining stepover and double-bend section remains horizontal,but their strikes change significantly with the increment of fault displacement.Thus,fractures intersecting the strike-slip direction at a small angle will become principal fractures early on,while those parallel to or anti-intersecting the strike-slip direction at a small angle will become principal fractures later.There are obvious differences in the development of fractures in different structural positions of strike-slip faults.Fractures are mainly concentrated in the fault tips,connections,and fault plane,and the magnitude of the fault damage zone is relatively larger in the first two.Compared with fault displacement,the principal damage zone(PDZ)shows stronger control on the distribution and development intensity of fractures.With the increment of fault displacement,the width of the fault damage zone and the fracture density first rapidly increases before the formation of PDZ and then slows down.Moreover,the formation time of PDZ in the restraining double-bend and stepover section is earlier than the simple strike-slip,releasing double-bend,and releasing stepover sections,and absorb more strain before the formation of the principal displacement zone.Thus,the restraining sections have the highest fracture intensity,followed by the pull-apart sections,then the simple strikeslip section.The results play an important role in understanding the development law of fractures related to strike-slip faults in different arrangements and move modes.展开更多
Imidacloprid is a neonicotinoid insecticide used for managing the Asian citrus psyllid,Diaphorina citri Kuwayama,which serves as vector of phytopathogens causing citrus greening.However,development of resistance to ne...Imidacloprid is a neonicotinoid insecticide used for managing the Asian citrus psyllid,Diaphorina citri Kuwayama,which serves as vector of phytopathogens causing citrus greening.However,development of resistance to neonicotinoids among populations of D.citri has coincided with occasional control failures in the field.The objectives of this research were to(1)survey current levels of imidacloprid resistance in Florida citrus;(2)compare feeding behavior between imidacloprid-resistant and susceptible D.citri using electrical penetration graph recordings,and(3)investigate the possible amplification of insecticide hormoligosis associated with resistance.Field surveys confirmed that the susceptibility of D.citri populations to imidacloprid has decreased in commercial Florida citrus groves compared with a laboratory-susceptible population.Following 12 generations of selection,resistance to imidacloprid increased by 438 fold compared with the susceptible strain.Imidacloprid-susceptible D.citri feeding on citrus exhibited significantly more bouts associated with intercellular pathway(C),phloem penetration(D),phloem salivation(E1),and nonprobing(Np)activities than imidacloprid-resistant counterparts.However,there were no differences observed in the frequency or duration of phloem ingestion or xylem feeding between susceptible and resistant D.citri.There was no statistical difference in fecundity between resistant and susceptible strains.However,the fecundity of imidacloprid-susceptible female D.citri treated with a sublethal concentration of imidacloprid(LC_(25))increased significantly compared with controls,while such hormoligosis was less pronounced among imidacloprid-resistant psyllids.Our results suggest that imidacloprid-resistant psyllids may cease feeding sooner than susceptible counterparts following sublethal exposure to this insecticide,indicative of a behavioral resistance mechanism.展开更多
基金supported by the National Natural Science Foundation of China(82271197,81974175,31820103006,32070971,32200792,32200776).
文摘Astrocyte maturation is crucial for brain function,yet the mechanisms regulating this process remain poorly understood.In this study,we identify the bHLH transcription factors Olig1 and Olig2 as essential coordinators of cortical astrocyte maturation.We demonstrate that Olig1 and Olig2 work synergistically to regulate cortical astrocyte maturation by modulating Bmp7 expression.Genetic ablation of both Olig1 and Olig2 results in defective astrocyte morphology,including reduced process complexity and an immature gene expression profile.Single-cell RNA sequencing reveals a shift towards a less mature astrocyte state,marked by elevated levels of HOPX and GFAP,resembling human astrocytes.Mechanistically,Olig1 and Olig2 bind directly to the Bmp7 enhancer,repressing its expression to promote astrocyte maturation.Overexpression of Bmp7 in vivo replicates the astrocyte defects seen in Olig1/2 double mutants,confirming the critical role of BMP7 signaling in this process.These findings provide insights into the transcriptional and signaling pathways regulating astrocyte development and highlight Olig1 and Olig2 as key regulators of cortical astrocyte maturation,with potential implications for understanding glial dysfunction in neurological diseases.
基金the financial support of the National Natural Science Foundation of China(Grant No.21975036)the Fundamental Research Funds for the Central Universities(Grant No.3132023503).
文摘Traditional metal sulfides used as anodes for sodium-ion batteries are hindered by sluggish kinetics,which limits their rate performance.Previous attempts to address this issue focused on nanostructured configurations with conductive frameworks.However,these nanomaterials often suffer from low packing density and the tendency for nanoparticles to agglomerate,posing significant challenges for practical applications.To overcome these limitations,this study presents a novel bimetal superionic anode material Cu_(3.21)Bi_(4.79)S_(9),which effectively resolves the conflict between sluggish kinetics and micrometer-scale particle size.By leveraging the vacancies created by free Cu and Bi atoms,this material forms rapid migration channels during sodium insertion and extraction,significantly reducing the migration barriers for sodium ions.The development of micrometer-scale Cu_(3.21)Bi_(4.79)S_(9)enables ultrafast chargingdischarging capabilities,achieving a reversible capacity of 325.5 mAh g^(-1)after 4000 cycles at a high rate of 45 C(15 A g^(-1)).This work marks a significant advancement in the field by offering a solution to the inherent trade-off between high capacity and rate performance in coarse-grained materials,reducing the need for reliance on nanostructured configurations for next-generation high-capacity anode materials.
基金This work was supported by grants from National Key Research and Development Program of China(2018YFA0108000)National Natural Science Foundation of China(31820103006,31630032,and 32070971)+1 种基金Shanghai Municipal Science and Technology Major Project(2018SHZDZX01)ZJ Lab,and Shanghai Center for Brain Science and Brain-Inspired Technology.We thank Dr.John L.Rubenstein for critical reading of the manuscript.
文摘Human cortical radial glial cells are primary neural stem cells that give rise to cortical glutaminergic projection pyramidal neurons, glial cells (oligodendrocytes and astrocytes) and olfactory bulb GABAergic interneurons. One of prominent features of the human cortex is enriched with glial cells, but there are major gaps in understanding how these glial cells are generated. Herein, by integrating analysis of published human cortical single-cell RNA-Seq datasets with our immunohistochemistical analyses, we show that around gestational week 18, EGFR-expressing human cortical truncated radial glial cells (tRGs) give rise to basal multipotent intermediate progenitors (bMIPCs) that express EGFR, ASCL1, OLIG2 and OLIG1. These bMIPCs undergo several rounds of mitosis and generate cortical oligodendrocytes, astrocytes and olfactory bulb interneurons. We also characterized molecular features of the cortical tRG. Integration of our findings suggests a general picture of the lineage progression of cortical radial glial cells, a fundamental process of the developing human cerebral cortex.
基金supported by grants from the National Key Research and Development Program of China(2018YFA0108000)the National Natural Science Foundation of China(31630032,31820103006,and 32070971)+1 种基金a Shanghai Municipal Science and Technology Major Project(2018SHZDZX01)ZJLab,and grants from NIH(R01MH094589 and R01NS089777)。
文摘Mouse cortical radial glial cells(RGCs)are primary neural stem cells that give rise to cortical oligodendrocytes,astrocytes,and olfactory bulb(OB)GABAergic interneurons in late embryogenesis.There are fundamental gaps in understanding how these diverse cell subtypes are generated.Here,by combining single-cell RNA-Seq with intersectional lineage analyses,we show that beginning at around E16.5,neocortical RGCs start to generate ASCL1^(+)EGFR^(+)apical multipotent intermediate progenitors(MIPCs),which then differentiate into basal MIPCs that express ASCL1,EGFR,OLIG2,and MKI67.These basal MIPCs undergo several rounds of divisions to generate most of the cortical oligodendrocytes and astrocytes and a subpopulation of OB interneurons.Finally,single-cell ATAC-Seq supported our model for the genetic logic underlying the specification and differentiation of cortical glial cells and OB interneurons.Taken together,this work reveals the process of cortical radial glial cell lineage progression and the developmental origins of cortical astrocytes and oligodendrocytes.
基金This work is supported by the National Natural Science Foundation of China(Nos.52175050 and 52205059)the Outstanding Youth Science Foundation(No.51922093)+1 种基金the Scientific Research Fund of Zhejiang Provincial Education Department(No.Y202148352)the Graduate Innovation Special Fund Project of Jiangxi Province(No.YC2021-B031),China.
文摘Locomotion stability is essential for controlling quadruped robots and adapting them to unstructured terrain.We propose a control strategy with center-of-mass(CoM)dynamic planning for the stable locomotion of these robots.The motion trajectories of the swing legs are synchronized with the CoM of the robot.To implement the synchronous control scheme,we adjusted the swing legs to form a support triangle.The strategy is applicable to both static walk gait and dynamic trot gait.In the motion control processes of the robot legs,the distribution of the ground reaction forces is optimized to minimize joint torque and locomotion energy consumption.We also used an improved joint-torque controller with varied controller coefficients in the stance and swing phases.The simulation and experimental results demonstrate that the robot can complete omnidirectional locomotion in both walk and trot gaits.At a given locomotion speed,the stability margins for the robot during walking and trotting were 27.25%and 37.25%higher,respectively,than in the scheme without CoM planning.The control strategy with energy consumption optimization(ECO)reduced the energy consumption of the robot in walk and trot gaits by 11.25%and 13.83%,respectively,from those of the control scheme without ECO.
文摘This paper discusses H-infinity state feedback control for a networked control system with time-varying delays. Based on the flee-weighing matrix method, a dehy-dependent stability criterion satisfying a prescribed H-infinity norm bound is presented for an NCS with unknown, time-varying and bounded delays. And then, the criterion is transformed into sufficient conditions based on linear matrix inequalities for H-infinity control. The conditions thus obtained are also used to design an H-infinity state feedback controller. This design method is further extended to solve the design problem of robust H-infinity state feedback control. A numerical example demonstrates the validity of the method.
基金supported in part by the National Natural Science Foundation of China(61333003,61690212)
文摘This paper investigates the remote tracking control problem of Network-based Agents with communication delays existing in both forward and feedback communication channels.A networked predictive tracking controller is proposed to compensate the negative effects caused by bilateral time-delays in a wireless network.Furthermore,the problem of consecutive data loss in the feedback channel is solved using aforementioned controller,where lateral movement perturbations are introduced.Simulations and experiments are provided for several cases,which verify the realizability and effectiveness of the proposed controller.
基金supported by the National Science and Technology Major Project(Grant No.2017ZX05009001-002 and 2017ZX05013002-004)the Fundamental Research Funds for the Central Universities(Grant No.2462020YJRC005)Science Foundation of China University of Petroleum,Beijing(Grant No.2462020XKJS02).
文摘Fractures are of great significance to tight oil and gas development.Fracture identification using conventional well logs is a feasible way to locate the underground fractures in tight sandstones.However,there are three problems affecting its interpretation accuracy and practical application,namely weak well log responses of fractures,a lack of specific logs for fracture prediction,and relative change omission in log responses.To overcome these problems and improve fracture identification accuracy,a fracture indicating parameter(FIP)method composed of a comprehensive index method(CIM)and a comprehensive fractal method(CFM)is introduced.The CIM tries to handle the first problem by amplifying log responses of fractures.The CFM addresses the third one using fractal dimensions.The flexible weight parameters corresponding to logs in the CIM and CFM make the interpretation possible for wells lacking specific logs.The reconstructed logs in the CIM and CFM try to solve the second problem.It is noted that the FIP method can calculate the probability of fracture development at a certain depth,but cannot show the fracture development degree of a new well compared with other wells.In this study,a formation fracture intensity(FFI)method is also introduced to further evaluate fracture development combined with production data.To test the validity of the FIP and FFI methods,fracture identification experiments are implemented in a tight reservoir in the Ordos Basin.The results are consistent with the data of rock core observation and production,indicating the proposed methods are effective for fracture identification and evaluation.
文摘Chronic occlusion of large intracranial arteries is the main cause of ischemic stroke in China.Patients with symptomatic intracranial artery occlusion and hemodynamic impairment are at high risk of recurrent stroke.Chronic occlusion of the intracranial segment of the internal carotid artery is a common type of intracranial artery occlusion.Medical management is regarded as the standard treatment for this disease.With the development of endovascular treatment,some patients with chronic cerebral artery occlusion have achieved satisfactory results with endovascular therapy.We reported a patient with symptomatic chronic occlusion of the ophthalmic segment of the internal carotid artery.Simple balloon angioplasty was performed,and the occluded ophthalmic segment of the internal carotid artery was successfully recanalized without perioperative complications.At 4 months followup,the internal carotid artery remained patent and perfusion of the right cerebral hemisphere improved dramatically.In addition,we briefly reviewed the relevant literature.
基金support from the Tangshan Talent Funding Project(A202202007)the National Natural Science Foundation of China(21703065)+1 种基金the Natural Science Foundation of Hebei Province(B2018209267,E2022209039)the Basic Science Center Program for Ordered Energy Conversion of the National Natural Science Foundation of China(51888103).
文摘Molybdenum disulfide(MoS_(2))has garnered significant attention in the field of catalysis due to the high density of active sites in its unique two-dimensional(2D)structure,which could be developed into numerous high-performance catalysts.The synthesis of ultra-small MoS2 particles(<10 nm)is highly desired in various experimental studies.The ultra-small structure could often lead to a distinct S-Mo coordination state and nonstoichiometric composition in MoSx,minimizing in-plane active sites of the 2D structure and making it probable to regulate the atomic and electronic structure of its intrinsic active sites on a large extent,especially in MoSx clusters.This article summarizes the recent progress of catalysis over ultra-small undoped MoS_(2) particles for renewable fuel production.Through a systematic review of their synthesis,structural,and spectral characteristics,as well as the relationship between their catalytic performance and inherent defects,we aim to provide insights into catalysis over this matrix that may potentially enable advancement in the development of high-performance MoS_(2)-based catalysts for sustainable energy generation in the future.
基金Supported by the National Natural Science Foundation of China[the Patterns Differentiation Mode of Main TCM Clinical Symptoms Based on Complex System Method(No.81270050)Information Extraction From TCM Inquiry and the Deducting Method of Patterns Differentiation Based on Feature Selection(No.30901897)+2 种基金Common Syndrome Diagnosis of Traditional Chinese Medicine Based on The Integration of Four Diagnosis Methods(No.81173199)]College Students' Scientific Innovation Foundation of Shanghai University of TCM[SHUTCMCXHDZ(2011)03]the Foundation for Training Talents of National Basic Scientific Research(No.J1103607)
文摘OBJECTIVE: Apply spectral clustering to analyze the patterns of symptoms in patients with chronic gastritis(CG).METHODS: Based on 919 CG subjects, we applied mutual information feature selection to choose the positively correlated symptoms with each pattern.Then, we used the Shi and Malik spectral clustering algorithm to select the top 20 correlated symptoms.RESULTS: We ascertained the results of six patterns.There were three categories for the pattern of accumulation of damp heat in the spleen-stomach(0.00332). There were six categories for the pattern of dampness obstructing the spleen-stomach(0.02466). There were two categories for the pattern of spleen-stomach Qi deficiency(0.013 89).There were three categories for the pattern of spleen-stomach deficiency cold(0.009 15). There were five categories for the pattern of liver-Qistagnation(0.01910).There were four categories for the pattern of stagnant heat in the liver-stomach(0.00585).CONCLUSION: Most of the spectral clustering results of the symptoms of CG patterns were in accordance with clinical experience and Traditional Chinese Medicine theory. Most categories suggested the nature and/or location of the disease.
基金the National Key Research and Development Program of China(2018YFAO 108000)the National Natural Science Foundation of China(31630032,81974175,and 31820103006)the Shanghai Municipal Science and Technology Major Project(2018SHZDZX01).
文摘Medium spiny neurons(MSNs)in the striatum,which can be divided into D1 and D2 MSNs,originate from the lateral ganglionic eminence(LGE).Previously,we reported that Six3 is a downstream target of Sp8/Sp9 in the transcriptional regulatory cascade of D2 MSN development and that conditionally knocking out Six3 leads to a severe loss of D2 MSNs.Here,we showed that Six3 mainly functions in D2 MSN precursor cells and gradually loses its function as D2 MSNs mature.Conditional deletion of Six3 had little effect on cell proliferation but blocked the differentiation of D2 MSN precursor cells.In addition,conditional overexpression of Six3 promoted the differentiation of precursor cells in the LGE.We measured an increase of apoptosis in the postnatal striatum of conditional Six3-knockout mice.This suggests that,in the absence of Six3,abnormally differentiated D2 MSNs are eliminated by programmed cell death.These results further identify Six3 as an important regulatory element during D2 MSN differentiation.
基金supported by the Young Scholars Developing Fund of Tangshan Teacher’s College (No.06D06)the Doctoral Fund of the Schoolthe Outstanding Overseas Chinese Scholars Fund of Chinese Academy of Sciences (No.2003-1-10)
文摘A theoretical model of a humidifier of proton exchange membrane （PEM） fuel cell systems is developed and analyzed first in this paper. The model shows that there exists a strong nonlinearity in the system. Then, the system is identified using a wavelet networks method. To avoid the curse-of-dimensionality problem, a class of wavelet networks proposed by Billings is employed. The experimental data acquired from the test bench are used for identification. The one-step-ahead predictions and the five-step-ahead predictions are compared with the real measurements, respectively. It shows that the identified model can effectively describe the real system.
文摘Dear Editor,This letter presents a novel data-driven trajectory planning and control scheme for the unmanned ground vehicles(UGVs).A recent work[1]has demonstrated the effectiveness of approximating the optimal state feedback for a nonlinear unmanned system via deep neural network(DNN).
基金Supported by National Natural Science Foundation of China(36120006)University-enterprise Cooperation Project(34120016)
文摘[Objectives] This study was conducted to illustrate the possible effects of heredity on the growth,reproduction and immune traits of Enshi Black pig.[Methods]The main growth,reproduction and immune traits of three populations( Goutou type,Shizitou type and an intermediate type between them) of Enshi Black pig were surveyed,and the correlations among these traits were analyzed.[Results]The health score of pregnant sows,average litter size and weight gain of piglets of intermediate type were all very significantly higher than those of Goutou and Shizitou types( P < 0.01).The percentage of lymphocytes of intermediate type was significantly higher than that of Goutou and Shizitou types( P < 0.05).The number of white blood cells( WBCs) and the number of neutrophils of intermediate type very were significantly higher than those of the other two types( P < 0.01).The percentages of CD3 + CD4 + double positive T cells( helper/inducer T lymphocytes) and CD3 + CD8 + double positive T cells( suppressor/cytotoxic T lymphocytes) and the CD4 +/CD8 + ratio( the ratio of helper T cells/suppressor T cells) of intermediate type were all significantly higher than those of the other two types.Correlation analysis showed that there was a very significant positive correlation between health score and litter size( P < 0.01,R = 0.336),a significant negative correlation between the percentage of lymphocytes and the number of neutrophils( P < 0.05,R =-0.733),and a significant correlation between the percentage of CD3 + CD8 + T cells and the number of platelets( P < 0.05,R = 0.700).[Conclusions] The intermediate type Enshi Black pig inherits the excellent traits of both Goutou type and Shizitou types,and thus has better growth,reproduction and immune traits than the other two types.
基金supported by the Shandong Provincial Natural Science Foundation(Grant Number ZR2021MH321).
文摘Background Although stent-assisted coiling has become a standard approach for treating intracranial aneurysms(IAs),there are limited reports on its safety and effectiveness in parent artery less than 2.5 mm in diameter.This study evaluates the feasibility,safety,and short-term outcomes of using Neuroform Atlas stent-assisted coiling for IAs with small parent vessels.Methods This study reviewed and analyzed the clinical data of 50 IAs in 50 patients with a parent artery diameter of≤2.5 mm,treated with Neuroform Atlas stent-assisted coiling at a single center between November 2020 and April 2024.Immediate postoperative angiographic outcomes were assessed using the modified Raymond-Roy classification.Follow-up imaging included computed tomographic angiography(CTA),magnetic resonance angiography(MRA),and digital subtraction angiography(DSA).Clinical outcomes were evaluated using the modified Rankin Scale(mRS).Results The procedures achieved a 100%success rate.Immediately after treatment,24 cases were classified as Raymond-Roy grade I,11 as grade II,and 15 as grade III.Follow-up angiography in 28 cases revealed three instances of aneurysm recurrence,with a secondary procedure performed in one case.One patient reported poor neurological status,and two cases experienced procedure-related adverse events during telephone or clinical follow-up.Conclusions the Atlas stent demonstrated favorable outcomes in the treatment of aneurysms in small parent arteries(<2.5 mm),with a low complication rate.The timely postoperative use of tirofiban may further reduce the risk of ischemic complications.
基金supported by the National Natural Science Foundation of China(81290342,81501757)the Special National Project on Research and Development of Key Biosafety Technologies(2016YFC1201904)+3 种基金the National Key Plan for Scientific Research and Development of China(2016YFD0500300)the Development Grants of State Key Laboratory of Infectious Disease Prevention and Control(2014SKLID103,2015SKLID505)the National Key Research and Development Program of China(2017YFC1200202)the Open Research Fund Program of Wuhan National Bio-Safety Level 4 Lab of CAS(2017SPCAS003)
文摘A field investigation of arboviruses was conducted in Dejiang, Guizhou Province in the summer of 2016. A total of 8,795 mosquitoes, belonging to four species of three genera, and 1,300 midges were collected. The mosquito samples were identified on site according to their morphology, and the pooled samples were ground and centrifuged in the laboratory. The supernatant was incubated with mosquito tissue culture cells(C6/36) and mammalian cells(BHK-21) for virus isolation. The results indicated that 40%(3,540/8,795) were Anopheles sinensis, 30%(2,700/8,795) were Culex pipiens quinquefasciatus, and 29%(2,530/8,795)were Armigeres subbalbeatus. Furthermore, a total of eight virus isolates were obtained, and genome sequencing revealed two Zika viruses(ZIKVs) isolated from Culex pipiens quinquefasciatus and Armigeres subbalbeatus, respectively; three Japanese encephalitis viruses(JEVs) isolated from Culex pipiens quinquefasciatus; two Banna viruses(BAVs) isolated from Culex pipiens quinquefasciatus and Anopheles sinensis, respectively; and one densovirus(DNV) isolated from Culex pipiens quinquefasciatus.The ZIKVs isolated from the Culex pipiens quinquefasciatus and Armigeres subbalbeatus mosquitoes represent the first ZIKV isolates in China's Mainland. This discovery presents new challenges for the prevention and control of ZIKV in China, and prompts international cooperation on this global issue.
基金This work was supported by National Natural Science Foundation of China(81871805,81925027 and 32130059)Jiangsu Provincial Clinical Orthopedic Center,Key Laboratory of Stem Cells and Biomedical Materials of Jiangsu Province and Chinese Ministry of Science and Technology,the Priority Academic Program Development(PAPD)of Jiangsu Higher Education Institutions.
文摘The dynamic extracellular matrix(ECM)constantly affects the behaviors of cells.To mimic the dynamics of ECM with controllable stiffness and energy dissipation,this study proposes a strategy in which a small molecule,3,4-dihydroxybenzaldehyde(DB),was used as fast"dynamic bridges"to construct viscoelastic gelatin methacryloyl(GelMA)-based hydrogels.The storage modulus and loss modulus of hydrogels were independently adjusted by the covalent crosslinking density and by the number of dynamic bonds.The hydrogels exhibited self-healing property,injectability,excellent adhesion and mechanical properties.Moreover,the in vitro results revealed that the viscous dissipation of hydrogels favored the spreading,proliferation,osteogenesis and chondrogenesis of bone marrow mesenchymal stem cells(BMSCs),but suppressed their adipogenesis.RNA-sequencing and immunofluorescence suggested that the viscous dissipation of hydrogels activated Yes-associated protein(YAP)by stabilizing integrinβ1,and further promoted nuclear translocation of smad2/3 andβ-catenin to enhance chondrogenesis and osteogenesis.As a result,the viscoelastic GelMA hydrogels with highest loss modulus showed best effect in cartilage and subchondral bone repair.Taken together,findings from this study reveal an effective strategy to fabricate viscoelastic hydrogels for modulating the interactions between cells and dynamic ECM to promote tissue regeneration.
基金This work was financially supported by the National Natural Science Foundation of China(Grant No.U21B2062)the 2021 American Association of Petroleum Geologists Foundation Grants-in-Aid Program.
文摘The fracture-cave reservoirs controlled by strike-slip faults are the main targets for oil and gas exploration of ultradeep carbonate in the Tarim Basin.It is of great significance to clarify the distribution rules of fractures related to strike-slip faults for guiding the exploration and development of ultra-deep oil and gas.In this study,six groups of strike-slip fault 3D models based on discrete element numerical simulation method have been created to investigate characteristics of fault-related fracture development and distribution law.In addition,we compared the modeling results to the measurement of fractures from the outcrop of a strike-slip fault in the Northern Tarim Basin to verify their validity.The results show that the stress environment is stable in the simple strike-slip section,and fractures intersecting with the strike-slip direction at a small angle are the principal fracture.In the releasing stepover and double-bend sections,the maximum principal stress changes from horizontal to vertical during the formation of pull-apart zones,where the principal fractures intersect the strike-slip direction at a large angle.The maximum principal stress in the restraining stepover and double-bend section remains horizontal,but their strikes change significantly with the increment of fault displacement.Thus,fractures intersecting the strike-slip direction at a small angle will become principal fractures early on,while those parallel to or anti-intersecting the strike-slip direction at a small angle will become principal fractures later.There are obvious differences in the development of fractures in different structural positions of strike-slip faults.Fractures are mainly concentrated in the fault tips,connections,and fault plane,and the magnitude of the fault damage zone is relatively larger in the first two.Compared with fault displacement,the principal damage zone(PDZ)shows stronger control on the distribution and development intensity of fractures.With the increment of fault displacement,the width of the fault damage zone and the fracture density first rapidly increases before the formation of PDZ and then slows down.Moreover,the formation time of PDZ in the restraining double-bend and stepover section is earlier than the simple strike-slip,releasing double-bend,and releasing stepover sections,and absorb more strain before the formation of the principal displacement zone.Thus,the restraining sections have the highest fracture intensity,followed by the pull-apart sections,then the simple strikeslip section.The results play an important role in understanding the development law of fractures related to strike-slip faults in different arrangements and move modes.
基金supported by a grant from the Citrus Research and Development Foundation and USDA APHIS MAC funding to LLS。
文摘Imidacloprid is a neonicotinoid insecticide used for managing the Asian citrus psyllid,Diaphorina citri Kuwayama,which serves as vector of phytopathogens causing citrus greening.However,development of resistance to neonicotinoids among populations of D.citri has coincided with occasional control failures in the field.The objectives of this research were to(1)survey current levels of imidacloprid resistance in Florida citrus;(2)compare feeding behavior between imidacloprid-resistant and susceptible D.citri using electrical penetration graph recordings,and(3)investigate the possible amplification of insecticide hormoligosis associated with resistance.Field surveys confirmed that the susceptibility of D.citri populations to imidacloprid has decreased in commercial Florida citrus groves compared with a laboratory-susceptible population.Following 12 generations of selection,resistance to imidacloprid increased by 438 fold compared with the susceptible strain.Imidacloprid-susceptible D.citri feeding on citrus exhibited significantly more bouts associated with intercellular pathway(C),phloem penetration(D),phloem salivation(E1),and nonprobing(Np)activities than imidacloprid-resistant counterparts.However,there were no differences observed in the frequency or duration of phloem ingestion or xylem feeding between susceptible and resistant D.citri.There was no statistical difference in fecundity between resistant and susceptible strains.However,the fecundity of imidacloprid-susceptible female D.citri treated with a sublethal concentration of imidacloprid(LC_(25))increased significantly compared with controls,while such hormoligosis was less pronounced among imidacloprid-resistant psyllids.Our results suggest that imidacloprid-resistant psyllids may cease feeding sooner than susceptible counterparts following sublethal exposure to this insecticide,indicative of a behavioral resistance mechanism.
