To investigate the influence of illumination on the fermentative hydrogen production system, the hydro- gen production efficiencies of two kinds of anaerobic activated sludge （floc and granule） from an anaerobic baf...To investigate the influence of illumination on the fermentative hydrogen production system, the hydro- gen production efficiencies of two kinds of anaerobic activated sludge （floc and granule） from an anaerobic baffled reactor were detected under visible light, dark and light-dark, respectively. The 10 mL floc sludge or granular sludge was respectively inoculated to 100 mL diluted molasses （chemical oxygen demand of 8000 mg.L^-1） in a 250 mL serum bottle, and cultured for 24 h at 37℃ under different illumination conditions. The results showed that the floc was more sensitive to illumination than the granule. A hydrogen yield of 19.8 mL was obtained in the dark with a specific hydrogen production rate of 3.52mol.kgLMLVSS.d^-1 （floc）, which was the highest among the three illumination conditions. Under dark condition, the hydrogen yield of floc sludge reached the highest with the specific hydrogen production rate of 3.52mol.kg^-1MLVSS.d^-1, and under light-dark, light, the specific hydrogen production rate was 3.11 and 2.21mol.kg^-1 MLVSS.d-1, respectively. The results demonstrated that the illumination may affect the dehydrogenase activity of sludge as well as the activity of hydrogen-producing acetogens and then impact hydrogen production capacity.展开更多
文摘To investigate the influence of illumination on the fermentative hydrogen production system, the hydro- gen production efficiencies of two kinds of anaerobic activated sludge （floc and granule） from an anaerobic baffled reactor were detected under visible light, dark and light-dark, respectively. The 10 mL floc sludge or granular sludge was respectively inoculated to 100 mL diluted molasses （chemical oxygen demand of 8000 mg.L^-1） in a 250 mL serum bottle, and cultured for 24 h at 37℃ under different illumination conditions. The results showed that the floc was more sensitive to illumination than the granule. A hydrogen yield of 19.8 mL was obtained in the dark with a specific hydrogen production rate of 3.52mol.kgLMLVSS.d^-1 （floc）, which was the highest among the three illumination conditions. Under dark condition, the hydrogen yield of floc sludge reached the highest with the specific hydrogen production rate of 3.52mol.kg^-1MLVSS.d^-1, and under light-dark, light, the specific hydrogen production rate was 3.11 and 2.21mol.kg^-1 MLVSS.d-1, respectively. The results demonstrated that the illumination may affect the dehydrogenase activity of sludge as well as the activity of hydrogen-producing acetogens and then impact hydrogen production capacity.
