Objective:To explore the intraperitoneal free cancer cell(IFCC)detection value of negative enrichment and immune fluorescence in situ hybridization(NEimFISH)on chromosomes(CEN)8/17.Methods:To verify the reliability of...Objective:To explore the intraperitoneal free cancer cell(IFCC)detection value of negative enrichment and immune fluorescence in situ hybridization(NEimFISH)on chromosomes(CEN)8/17.Methods:To verify the reliability of NEimFISH,29 gastric cancer tumors,their adjacent tissues and greater omental tissues were tested.Our study then included 105 gastric cancer patients for IFCC.We defined patients as IFCC-positive if a signal was detected,regardless of the detailed cancer cell numbers.A comparison of clinicopathological features was conducted among IFCC groups.We also compared the diagnosis value and peritoneal recurrence predictive value among different detection methods.The comparison of IFCC number was also conducted among different groups.Results:A cutoff of 2.5 positive cells could distinguish all benign tissue samples and 97%of malignant tissue samples in our study.Compared to intestinal gastric cancer,patients with diffuse gastric cancer tended to have more IFCCs(6 vs.4,P=0.002).The IFCC counts were often higher in the lymphovascular invasion positive group than negative group(3 vs.1,P=0.022).All IFCC samples that were considered positive using conventional cytology were also found to be positive using NEimFISH.When compared to conventional cytology and paraffin pathology,NEimFISH had a higher IFCC positive rate(68.9%)and higher one-year peritoneal recurrence predictive value with area under the curve(AUC)of 0.922.Conclusions:Gastric cancer could be effectively diagnosed by NEimFISH.The IFCC number found using NEimFISH on CEN8/17 is closely associated with Lauren type and vascular invasion of cancer.NEimFISH is a reliable detection modality with a higher positive detection rate,higher one-year peritoneal recurrence predictive value and quantitative features for IFCC of gastric cancer.展开更多
Objective： To understand distribution and drug resistance of pathogenic bacteria from a specialized cancer hospital in 2013 in order to provide a basis for rational clinical antimicrobial agents. Methods： Pathogenic...Objective： To understand distribution and drug resistance of pathogenic bacteria from a specialized cancer hospital in 2013 in order to provide a basis for rational clinical antimicrobial agents. Methods： Pathogenic bacteria identification and drug sensitivity tests were performed with a VITEK 2 compact automatic identification system and data were analyzed using WHONET5.6 software.Results： Of the 1,378 strains tested, 980 were Gram-negative bacilli, accounting for 71.1%, in which Klebsiella pneumonia, Escherichia coli and Pseudomonas aeruginosa were the dominant strains. We found 328 Gram-positive coccus, accounting for 23.8%, in which the amount of Staphylococcus aureus was the highest. We identified 46 fungi, accounting for 4.1%. According to the departmental distribution within the hospital, the surgical departments isolated the major strains, accounting for 49.7%. According to disease types, lung cancer, intestinal cancer and esophagus cancer were the top three, accounting for 20.9%, 17.3% and 14.2%, respectively. No strains were resistant to imipenem, ertapenem or vancomycin.Conclusions： Pathogenic bacteria isolated from the specialized cancer hospital have different resistance rates compared to commonly used antimicrobial agents; therefore antimicrobial agents to reduce the morbidity and mortality of infections should be used.展开更多
Triethylamine(TEA),1,4-dibromobutane(C4H8Br2)and N,N-dimethylaminopropyl acrylamide(DMAPAA)were selected to synthesize a double quaternary ammonium cationic monomer(TDD).TDD,acrylamide(AM),2-acrylamido-2-methylpropane...Triethylamine(TEA),1,4-dibromobutane(C4H8Br2)and N,N-dimethylaminopropyl acrylamide(DMAPAA)were selected to synthesize a double quaternary ammonium cationic monomer(TDD).TDD,acrylamide(AM),2-acrylamido-2-methylpropane sulfonic acid(AMPS)and acrylic acid(AA)were used to create a quadripolymer(PAAT)that was characterized by FT-IR and ^(1)H NMR.Following that,the basic performance of thickened acid was assessed.The results revealed that PAAT outperformed PAA(copolymerized by AM,AMPS and AA)in terms of acid solubility,thickening performance,temperature resistance and shear resistance.Furthermore,this crosslinked acid containing PAAT was subjected to a series of performance evaluations.The viscosity of the crosslinked acid could be kept at 67 mPa$s at 160C after 90 min and the weight loss rate of crosslinked acid in acid-rock reaction at 60 min was 23%,indicating the temperature resistance and retarder performance of the crosslinked acid solution was significantly better than that of the thickening acid and the blank acid.A scanning electron microscopy experiment revealed that crosslinked acid has good corrosion ability and low damage to the reservoir.The above experimental results indicated that the thickener PAAT has a promising future application in acid fracturing of high-temperature and high-mineralization carbonate reservoirs.展开更多
基金supported by the National Science Foundation for Young Scientists of China (No. 81802735)
文摘Objective:To explore the intraperitoneal free cancer cell(IFCC)detection value of negative enrichment and immune fluorescence in situ hybridization(NEimFISH)on chromosomes(CEN)8/17.Methods:To verify the reliability of NEimFISH,29 gastric cancer tumors,their adjacent tissues and greater omental tissues were tested.Our study then included 105 gastric cancer patients for IFCC.We defined patients as IFCC-positive if a signal was detected,regardless of the detailed cancer cell numbers.A comparison of clinicopathological features was conducted among IFCC groups.We also compared the diagnosis value and peritoneal recurrence predictive value among different detection methods.The comparison of IFCC number was also conducted among different groups.Results:A cutoff of 2.5 positive cells could distinguish all benign tissue samples and 97%of malignant tissue samples in our study.Compared to intestinal gastric cancer,patients with diffuse gastric cancer tended to have more IFCCs(6 vs.4,P=0.002).The IFCC counts were often higher in the lymphovascular invasion positive group than negative group(3 vs.1,P=0.022).All IFCC samples that were considered positive using conventional cytology were also found to be positive using NEimFISH.When compared to conventional cytology and paraffin pathology,NEimFISH had a higher IFCC positive rate(68.9%)and higher one-year peritoneal recurrence predictive value with area under the curve(AUC)of 0.922.Conclusions:Gastric cancer could be effectively diagnosed by NEimFISH.The IFCC number found using NEimFISH on CEN8/17 is closely associated with Lauren type and vascular invasion of cancer.NEimFISH is a reliable detection modality with a higher positive detection rate,higher one-year peritoneal recurrence predictive value and quantitative features for IFCC of gastric cancer.
基金supported by the National Key Research&Development Program of China(no.2016YFC0401904)the Science Fund for Creative Research Groups of the National Natural Science Foundation of China(no.51621092)+1 种基金the National Natural Science Foundation of China(nos.51309177 and 51409187)the Program of Introducing Talents of Discipline to Universities(No.B14012)
文摘Objective： To understand distribution and drug resistance of pathogenic bacteria from a specialized cancer hospital in 2013 in order to provide a basis for rational clinical antimicrobial agents. Methods： Pathogenic bacteria identification and drug sensitivity tests were performed with a VITEK 2 compact automatic identification system and data were analyzed using WHONET5.6 software.Results： Of the 1,378 strains tested, 980 were Gram-negative bacilli, accounting for 71.1%, in which Klebsiella pneumonia, Escherichia coli and Pseudomonas aeruginosa were the dominant strains. We found 328 Gram-positive coccus, accounting for 23.8%, in which the amount of Staphylococcus aureus was the highest. We identified 46 fungi, accounting for 4.1%. According to the departmental distribution within the hospital, the surgical departments isolated the major strains, accounting for 49.7%. According to disease types, lung cancer, intestinal cancer and esophagus cancer were the top three, accounting for 20.9%, 17.3% and 14.2%, respectively. No strains were resistant to imipenem, ertapenem or vancomycin.Conclusions： Pathogenic bacteria isolated from the specialized cancer hospital have different resistance rates compared to commonly used antimicrobial agents; therefore antimicrobial agents to reduce the morbidity and mortality of infections should be used.
基金The authors appreciate the financial support from the National Natural Science Foundation of China(U1762212)the Shandong Provincial Natural Science Foundation,China(ZR2019MEE037).
文摘Triethylamine(TEA),1,4-dibromobutane(C4H8Br2)and N,N-dimethylaminopropyl acrylamide(DMAPAA)were selected to synthesize a double quaternary ammonium cationic monomer(TDD).TDD,acrylamide(AM),2-acrylamido-2-methylpropane sulfonic acid(AMPS)and acrylic acid(AA)were used to create a quadripolymer(PAAT)that was characterized by FT-IR and ^(1)H NMR.Following that,the basic performance of thickened acid was assessed.The results revealed that PAAT outperformed PAA(copolymerized by AM,AMPS and AA)in terms of acid solubility,thickening performance,temperature resistance and shear resistance.Furthermore,this crosslinked acid containing PAAT was subjected to a series of performance evaluations.The viscosity of the crosslinked acid could be kept at 67 mPa$s at 160C after 90 min and the weight loss rate of crosslinked acid in acid-rock reaction at 60 min was 23%,indicating the temperature resistance and retarder performance of the crosslinked acid solution was significantly better than that of the thickening acid and the blank acid.A scanning electron microscopy experiment revealed that crosslinked acid has good corrosion ability and low damage to the reservoir.The above experimental results indicated that the thickener PAAT has a promising future application in acid fracturing of high-temperature and high-mineralization carbonate reservoirs.
