Objective To investigate the feasibility of acquiring the similar homogeneous enhancement using bolus-tracking techniques with shortened respiratory time in prospectively electrocardiogram-gated high-pitch spiral acqu...Objective To investigate the feasibility of acquiring the similar homogeneous enhancement using bolus-tracking techniques with shortened respiratory time in prospectively electrocardiogram-gated high-pitch spiral acquisition mode (Flash mode) coronary computed tomography angiography (CCTA) compared with test bolus technique. Methods One hundred and eighty-four consecutive patients with mean heart rate ≤65 beats per minute undergoing CCTA were prospectively included in this study. The patients were randomly divided into two groups. Patients in the group A (n=92) instructed to shorten respiratory time received CCTA using bolus-tracking technique with high-pitch spiral acquisition mode (Flash mode), while those in the group B (n=92) underwent CCTA with test bolus technique. The attenuation in the ascending aorta, image noise, contrast-to-noise ratio and radiation doses of the two groups were assessed. Results There were no significant differences in the mean attenuation values in the ascending aorta (483.18±59.07 HU vs. 498.7±83.51 HU, P=0.183), image noise (21.4±4.5 HU vs. 20.9±4.3 HU, P=0.414), contrast-to-noise ratio (12.1±4.2 vs. 13.8±5.1, P=0.31) between the groups A and B. There were no significant differences in the radiation dose of dynamic monitoring scans (0.056±0.026 mSv vs. 0.062±0.018 mSv, P=0.068) and radiation dose of angiography (0.94±0.07 mSv vs. 0.96±0.15 mSv,P=0.926) between the two groups, while 15 mL less contrast material volume was administered in the group A than the group B. Conclusion Bolus-tracking technique with shortened time of respiratory in Flash mode of dual-source CT yields the similar homogeneous enhancement with less contrast material in comparison to the test bolus technique.展开更多
Objective:To analyze the mechanism of HSP70 regulating endothelial cell injury in patients with acute sepsis.Methods:From February 2017 to December 2018,3 patients with acute sepsis in our hospital were selected as th...Objective:To analyze the mechanism of HSP70 regulating endothelial cell injury in patients with acute sepsis.Methods:From February 2017 to December 2018,3 patients with acute sepsis in our hospital were selected as the observation group,and 3 patients with fracture undergoing surgical treatment were selected as the control group.The endothelial cells were extracted and divided into blank subgroup,10 mg/L,50 mg/L and 100 mg/L subgroups.The cell viability of each group was detected by MTT,the nucleus morphology was observed by fluorescence microscope,and autophagosomes of endothelial cells were observed by transmission electron microscope,and then the level of Bcl-2,Beclin-1 andβ-actin protein expression were detected.Results:HSP70 intervention can effectively improve the endothelial cell vitality of patients with acute sepsis.The cell viability of 100 mg/L subgroup was the highest in the observation group and the control group,and the cell viability of the blank subgroup was the lowest,and the difference was statistically significant(P<0.05).Compared with the control group,the endothelial cell nuclear defect of acute sepsis patients was serious.HSP70 intervention can effectively improve the nuclear morphology,autophagy morphology and structural morphology,and the 100 mg/L subgroup had the best nuclear morphology and autophagy morphology.HSP70 intervention can effectively improve the levels of Bcl-2 and beclin-1 in endothelial cells of patients with acute sepsis.The levels of Bcl-2 and beclin-1 were the highest in the 100 mg/L subgroup of the observation group and the control group,and the lowest in the blank subgroup,and the difference was statistically significant(P<0.05).Conclusion:HSP70 can effectively regulate the level of Bcl-2 in endothelial cells of patients with acute sepsis,which effectively inhibit cell apoptosis and alleviate cell skin damage.展开更多
基金Supported by the Ministry of Science and Technology of Inner Mongolia, China (20110504)
文摘Objective To investigate the feasibility of acquiring the similar homogeneous enhancement using bolus-tracking techniques with shortened respiratory time in prospectively electrocardiogram-gated high-pitch spiral acquisition mode (Flash mode) coronary computed tomography angiography (CCTA) compared with test bolus technique. Methods One hundred and eighty-four consecutive patients with mean heart rate ≤65 beats per minute undergoing CCTA were prospectively included in this study. The patients were randomly divided into two groups. Patients in the group A (n=92) instructed to shorten respiratory time received CCTA using bolus-tracking technique with high-pitch spiral acquisition mode (Flash mode), while those in the group B (n=92) underwent CCTA with test bolus technique. The attenuation in the ascending aorta, image noise, contrast-to-noise ratio and radiation doses of the two groups were assessed. Results There were no significant differences in the mean attenuation values in the ascending aorta (483.18±59.07 HU vs. 498.7±83.51 HU, P=0.183), image noise (21.4±4.5 HU vs. 20.9±4.3 HU, P=0.414), contrast-to-noise ratio (12.1±4.2 vs. 13.8±5.1, P=0.31) between the groups A and B. There were no significant differences in the radiation dose of dynamic monitoring scans (0.056±0.026 mSv vs. 0.062±0.018 mSv, P=0.068) and radiation dose of angiography (0.94±0.07 mSv vs. 0.96±0.15 mSv,P=0.926) between the two groups, while 15 mL less contrast material volume was administered in the group A than the group B. Conclusion Bolus-tracking technique with shortened time of respiratory in Flash mode of dual-source CT yields the similar homogeneous enhancement with less contrast material in comparison to the test bolus technique.
基金Talent training plan of Shanghai Pudong New Area public interest hospital(No.GLRq2018-03)Key specialty of health system in Pudong New Area(No.PWZzk2017-05)Youth Science and technology project of health and family planning in Pudong New Area in 2018(No.PW2018B-03)。
文摘Objective:To analyze the mechanism of HSP70 regulating endothelial cell injury in patients with acute sepsis.Methods:From February 2017 to December 2018,3 patients with acute sepsis in our hospital were selected as the observation group,and 3 patients with fracture undergoing surgical treatment were selected as the control group.The endothelial cells were extracted and divided into blank subgroup,10 mg/L,50 mg/L and 100 mg/L subgroups.The cell viability of each group was detected by MTT,the nucleus morphology was observed by fluorescence microscope,and autophagosomes of endothelial cells were observed by transmission electron microscope,and then the level of Bcl-2,Beclin-1 andβ-actin protein expression were detected.Results:HSP70 intervention can effectively improve the endothelial cell vitality of patients with acute sepsis.The cell viability of 100 mg/L subgroup was the highest in the observation group and the control group,and the cell viability of the blank subgroup was the lowest,and the difference was statistically significant(P<0.05).Compared with the control group,the endothelial cell nuclear defect of acute sepsis patients was serious.HSP70 intervention can effectively improve the nuclear morphology,autophagy morphology and structural morphology,and the 100 mg/L subgroup had the best nuclear morphology and autophagy morphology.HSP70 intervention can effectively improve the levels of Bcl-2 and beclin-1 in endothelial cells of patients with acute sepsis.The levels of Bcl-2 and beclin-1 were the highest in the 100 mg/L subgroup of the observation group and the control group,and the lowest in the blank subgroup,and the difference was statistically significant(P<0.05).Conclusion:HSP70 can effectively regulate the level of Bcl-2 in endothelial cells of patients with acute sepsis,which effectively inhibit cell apoptosis and alleviate cell skin damage.
