目的探讨极速热循环荧光定量PCR系统在甲型流感病毒(influenza A virus,IAV)和乙型流感病毒(influenza B virus,IBV)检测中的性能。方法构建IAV和IBV的质粒标准品,浓度梯度稀释,每个浓度重复检测20次后,制作浓度标准曲线,评价系统的最...目的探讨极速热循环荧光定量PCR系统在甲型流感病毒(influenza A virus,IAV)和乙型流感病毒(influenza B virus,IBV)检测中的性能。方法构建IAV和IBV的质粒标准品,浓度梯度稀释,每个浓度重复检测20次后,制作浓度标准曲线,评价系统的最低检出限、线性范围和线性相关系数。收集来自南部战区疾病预防控制中心生物安全科2016年至2018年的临床样本278例(IAV阳性样本88例,IBV阳性样本87例,阴性样本103例),其中男性164人(59%),女性114人(41%),平均年龄24岁,通过重复检测相同IAV、IBV临床样本评价其分析重现性;将系统检测278例鼻/咽拭子临床样本与传统实时荧光定量PCR(real-time quantitative PCR,RT-qPCR)结果比较评价其效果。结果极速热循环荧光定量PCR系统检测IAV和IBV质粒标准品的最低检出限为1×10^(0)copies/μL;线性检测范围是1×10^(0)~1×10^(5)copies/μL;此系统检测流感临床样本的敏感度为92.57%,特异度均为100%,阴、阳性预测值分别为88.79%和100%,与RT-qPCR结果的符合率为95.32%;IAV与IBV之间以及IAV和IBV与其他多种病原体之间无交叉反应;30 g/dL的血红蛋白、3.2 g/dL的甘油三酯和100μg/mL头孢曲松对检测结果无干扰。结论极速热循环荧光定量PCR系统体积小、重量轻、无需核酸提取步骤、耗时短、检测性能良好,适用于即时检测(point-of-care testing,POCT)。展开更多
Nowadays, China has entered the aging countries sequence, reform and development of the social insurance system is facing more opportunities and challenges, which give the social insurance audit work of the new requir...Nowadays, China has entered the aging countries sequence, reform and development of the social insurance system is facing more opportunities and challenges, which give the social insurance audit work of the new requirements. In practice, the social insurance audit work has encountered new conflicts and problems. Due to the socio-economic composition, organizational forms, forms of employment, interest, distribution relations have had a dramatic and profound change, labor relations was diversified, contract, complex features, social insurance premium is difficult, concealed drain Daily contribution base, have occurred impersonator false and social insurance benefits behavior. Loss of the social insurance fund is now urgently containment dike, to further regulate the social insurance audit work has become an important issue in the New Social Insurance Development. Therefore, we must establish a sound social security audit system, and promote the integration of social security audits, and thus be able to maintain the smooth progress of China＇s social insurance work.展开更多
文摘目的探讨极速热循环荧光定量PCR系统在甲型流感病毒(influenza A virus,IAV)和乙型流感病毒(influenza B virus,IBV)检测中的性能。方法构建IAV和IBV的质粒标准品,浓度梯度稀释,每个浓度重复检测20次后,制作浓度标准曲线,评价系统的最低检出限、线性范围和线性相关系数。收集来自南部战区疾病预防控制中心生物安全科2016年至2018年的临床样本278例(IAV阳性样本88例,IBV阳性样本87例,阴性样本103例),其中男性164人(59%),女性114人(41%),平均年龄24岁,通过重复检测相同IAV、IBV临床样本评价其分析重现性;将系统检测278例鼻/咽拭子临床样本与传统实时荧光定量PCR(real-time quantitative PCR,RT-qPCR)结果比较评价其效果。结果极速热循环荧光定量PCR系统检测IAV和IBV质粒标准品的最低检出限为1×10^(0)copies/μL;线性检测范围是1×10^(0)~1×10^(5)copies/μL;此系统检测流感临床样本的敏感度为92.57%,特异度均为100%,阴、阳性预测值分别为88.79%和100%,与RT-qPCR结果的符合率为95.32%;IAV与IBV之间以及IAV和IBV与其他多种病原体之间无交叉反应;30 g/dL的血红蛋白、3.2 g/dL的甘油三酯和100μg/mL头孢曲松对检测结果无干扰。结论极速热循环荧光定量PCR系统体积小、重量轻、无需核酸提取步骤、耗时短、检测性能良好,适用于即时检测(point-of-care testing,POCT)。
文摘Nowadays, China has entered the aging countries sequence, reform and development of the social insurance system is facing more opportunities and challenges, which give the social insurance audit work of the new requirements. In practice, the social insurance audit work has encountered new conflicts and problems. Due to the socio-economic composition, organizational forms, forms of employment, interest, distribution relations have had a dramatic and profound change, labor relations was diversified, contract, complex features, social insurance premium is difficult, concealed drain Daily contribution base, have occurred impersonator false and social insurance benefits behavior. Loss of the social insurance fund is now urgently containment dike, to further regulate the social insurance audit work has become an important issue in the New Social Insurance Development. Therefore, we must establish a sound social security audit system, and promote the integration of social security audits, and thus be able to maintain the smooth progress of China＇s social insurance work.
