This study uses DEM (Digital Elevation Model) data and remote sensing maps of the study area in 1993, 1999, and 2009 to analyze the slope gradient change of land use patterns in Qiandongnan Prefecture, Guizhou provi...This study uses DEM (Digital Elevation Model) data and remote sensing maps of the study area in 1993, 1999, and 2009 to analyze the slope gradient change of land use patterns in Qiandongnan Prefecture, Guizhou province, China. The land use data were classified into five types, forest, farmland, grassland, water and built-up, the slope gradients were divided into four grades. Indices for analyzing land use features were defined by their proportions, transformation matrixes, land use degree and changes The results showed that all land use types can be found at every gradient. Generally, with the slope degree increased, the area of forest being augmented as well, while the area of the other land use types (farmland, grassland and build-up) declined. Moreover, a mass of farmland were shifted from other land use types from o° to 25°, while a quantity of forest were transformed from the other land use types on 〉 25° from 2993 to 2009. In terms of land use degree and changes, the area of farmland and buildup land use types decreased when slope degree increased. Finally, we calculated the five landscape pattern metrics: Patch Density (PD) value, Largest Patch Index (LPI), Shannon's Diversity Index (SHDI), Area-Weighted Mean Shape Index (AWMSI) and Contagion Index (CONTAG). The results of metricsanalysis showed that PD values, SHDI values and CONTA values had a similar variation trend, that is, they decreased when slope degree increased. There was no obvious variation trend on LPI value.展开更多
The 1st China Cartoon and Animation Art Exhibition was recently held in the National Art Museum of China, which was jointly hosted by ten ministries and commissions, including the Ministry of Culture, the Ministry of ...The 1st China Cartoon and Animation Art Exhibition was recently held in the National Art Museum of China, which was jointly hosted by ten ministries and commissions, including the Ministry of Culture, the Ministry of Finance, the State Administration of Radio, Film and TV, the State Administration of Press and Publication, the Ministry of Education,展开更多
Ubiquitination of proteins is one of the critical regulatory mechanisms in eukaryotes. In higher plants, protein ubiquitination plays an essential role in many biological processes, including hormone signaling, photom...Ubiquitination of proteins is one of the critical regulatory mechanisms in eukaryotes. In higher plants, protein ubiquitination plays an essential role in many biological processes, including hormone signaling, photomorphogenesis, and pathogen defense. However, the roles of protein ubiquitination in the repro- ductive process are not clear. In this study, we identified four plant-specific RING-finger genes designated Aberrant_Pollen Development 1_ (APD1) to APD4, as regulators of pollen mitosis II (PMII) in Arabidopsis thaliana (L.). The apdl apd2 double mutant showed a significantly increased percentage of bicellular-like pollen at the mature pollen stage. Further downregulation of the APD3 and APD4 transcripts in apdl apd2 by RNA interference (RNAi) resulted in more severe abnormal bicellular-like pollen phenotypes than in apdl apd2, suggesting that cell division was defective in male gametogenesis. All of the four genes were expressed in multiple stages at different levels during male gametophyte development. Confocal analysis using green florescence fusion proteins (GFP) GFP-APD1 and GFP-APD2 showed that APDs are associated with intracellular membranes. Furthermore, APD2 had E2-dependent E3 ligase activity in vitro, and five APD2-interacting proteins were identified. Our results suggest that these four genes may be involved, redundantly, in regulating the PMII process during male gametogenesis.展开更多
基金supported by the National Natural Science Foundation of China (Grant No. 31370480)111 Project (B08044)Minzu University of China (MUC98507-08)
文摘This study uses DEM (Digital Elevation Model) data and remote sensing maps of the study area in 1993, 1999, and 2009 to analyze the slope gradient change of land use patterns in Qiandongnan Prefecture, Guizhou province, China. The land use data were classified into five types, forest, farmland, grassland, water and built-up, the slope gradients were divided into four grades. Indices for analyzing land use features were defined by their proportions, transformation matrixes, land use degree and changes The results showed that all land use types can be found at every gradient. Generally, with the slope degree increased, the area of forest being augmented as well, while the area of the other land use types (farmland, grassland and build-up) declined. Moreover, a mass of farmland were shifted from other land use types from o° to 25°, while a quantity of forest were transformed from the other land use types on 〉 25° from 2993 to 2009. In terms of land use degree and changes, the area of farmland and buildup land use types decreased when slope degree increased. Finally, we calculated the five landscape pattern metrics: Patch Density (PD) value, Largest Patch Index (LPI), Shannon's Diversity Index (SHDI), Area-Weighted Mean Shape Index (AWMSI) and Contagion Index (CONTAG). The results of metricsanalysis showed that PD values, SHDI values and CONTA values had a similar variation trend, that is, they decreased when slope degree increased. There was no obvious variation trend on LPI value.
文摘The 1st China Cartoon and Animation Art Exhibition was recently held in the National Art Museum of China, which was jointly hosted by ten ministries and commissions, including the Ministry of Culture, the Ministry of Finance, the State Administration of Radio, Film and TV, the State Administration of Press and Publication, the Ministry of Education,
基金supported by the National Key Basic Research Program of the People's Republic of China(2011CB915402)the National Science Foundation of China(30970270to JJL)
文摘Ubiquitination of proteins is one of the critical regulatory mechanisms in eukaryotes. In higher plants, protein ubiquitination plays an essential role in many biological processes, including hormone signaling, photomorphogenesis, and pathogen defense. However, the roles of protein ubiquitination in the repro- ductive process are not clear. In this study, we identified four plant-specific RING-finger genes designated Aberrant_Pollen Development 1_ (APD1) to APD4, as regulators of pollen mitosis II (PMII) in Arabidopsis thaliana (L.). The apdl apd2 double mutant showed a significantly increased percentage of bicellular-like pollen at the mature pollen stage. Further downregulation of the APD3 and APD4 transcripts in apdl apd2 by RNA interference (RNAi) resulted in more severe abnormal bicellular-like pollen phenotypes than in apdl apd2, suggesting that cell division was defective in male gametogenesis. All of the four genes were expressed in multiple stages at different levels during male gametophyte development. Confocal analysis using green florescence fusion proteins (GFP) GFP-APD1 and GFP-APD2 showed that APDs are associated with intracellular membranes. Furthermore, APD2 had E2-dependent E3 ligase activity in vitro, and five APD2-interacting proteins were identified. Our results suggest that these four genes may be involved, redundantly, in regulating the PMII process during male gametogenesis.