The medicinal herb Geranium wallichianum belongs to the family Geraniaceae. The East Asian Himalayas are its primary habitat. Overexploitation and overharvesting pose a threat to this plant, given its extensive ethnom...The medicinal herb Geranium wallichianum belongs to the family Geraniaceae. The East Asian Himalayas are its primary habitat. Overexploitation and overharvesting pose a threat to this plant, given its extensive ethnomedical utilization in the community. In Pakistan, its population has already declined by over 75%. Given its critical medicinal importance, urgent conservation efforts are needed to prevent extinction. The aim of the current research was to determine the effectiveness of sucrose and polyethylene glycol (PEG) in decelerating the growth of this medicinally important species. Nodal segments were utilized as explant with varying levels of polyethylene glycol (PEG) (0.5% and 1.5%) and sucrose (4% and 8%) as treatments on different growth attributes. Over a 45-day period, PEG treatments (0.5% and 1.5%) proved more efficient compared to sucrose in suppressing in vitro growth, with reduced shoot lengths (0.92 and 0.57 cm), dry weight (0.04 and 0.02 g), and fresh weight (0.06 and 0.04 g). PEG also significantly enhanced phenolic levels (0.96 and 0.19 mg/g) and soluble sugars (4.07 and 4.12 mg/g) while reducing total protein level (2.38 and 2.32 mg/g) and total chlorophyll content (0.17 and 0.14 mg/g). The Pearson correlation analysis showed a negative association between phenolic levels and total chlorophyll content, chlorophyll a, and chlorophyll b. The length of the shoot had a negative relationship with phenolic level but a positive relationship with both fresh and dry weight. The principal component analysis revealed that total sugars and phenolics that were grouped separately from other variables led to stronger responses to osmotic stress. These outcomes suggested that higher PEG was successful in reducing in vitro growth throughout short-term preservation in comparison to sucrose. The outcomes of this research could be applied for the in vitro conservation of medical plants in the future.展开更多
Rosa centifolia is grown for its flowers which are used for essential oil extraction. It is vegetatively propagated through its cuttings which are difficult to root. This study was designed to increase the rooting per...Rosa centifolia is grown for its flowers which are used for essential oil extraction. It is vegetatively propagated through its cuttings which are difficult to root. This study was designed to increase the rooting percentage of cuttings by applying plant growth regulators (PGRs) with different levels. In total 31 treatments with different concentrations (450 ppm, 700 ppm and 950 ppm) of indole butyric acid (IBA), indole acetic acid (IAA), naphthalene acetic acid (NAA) alone, in combination and with same concentrations of 6-benzylamino purine (BAP) were used. Healthy similar sized cutting of R. centifolia was treated in solution of PGRs by quick dip method and planted in polythene bags. The data for shoot length, shoot dry weight, number of roots, root length and root dry weight were recorded. All three levels (450 ppm, 700 ppm and 950 ppm) of IBA alone produced maximum results in case of all parameters as compared to all other treatments. Among three levels of IBA, 450 ppm concentration produced maximum shoot length (10.67 cm), shoot dry weight (3.02 g), number of roots (14.00), root length (11.90 cm) and root dry weight (0.50 g). Lower concentrations of plant growth regulators produced better results as compared to higher concentrations.展开更多
Jujube is an underutilized fruit having high maturity variation at harvest.Investigations were made on eight different cultivars of jujube(Umran,Karela,Gurrh,Amrodia,Mehmood Wali,Aakash,Foladi and Khubani)for changes ...Jujube is an underutilized fruit having high maturity variation at harvest.Investigations were made on eight different cultivars of jujube(Umran,Karela,Gurrh,Amrodia,Mehmood Wali,Aakash,Foladi and Khubani)for changes in antioxidative enzymatic activities,phytochemical functions and proximate composition with respect to different harvest maturity stages(M_(1):mature green,M_(2):yellow and M_(3):crispy brown)under postharvest ambient conditions(25±2℃;60%-65%RH)at three shelf intervals(1,3 and 5 d).Significant genotypic and maturity related variation was recorded regarding studied attributes.The fruit of cv.ʽKhubaniʼhad better attributes among the cultivars evaluated.The crispy brown jujube fruit had higher peroxidase(POD)and catalase(CAT)activity and more protein,total soluble solids(TSS),ash,crude fat and fiber than early mature stages.Over time,the ascorbic acid(vitamin C),titratable acidity(TA),total phenolic(TPC),total antioxidant capacity(TAC),crude protein,crude fiber,moisture,crude fat and activity of antioxidative enzymes[CAT and superoxide dismutase(SOD)]exhibited a significant decline;while increase was recorded in TSS,pH and POD activity.Overall,this work identified that jujube’s antioxidative,biochemical and proximate properties are significantly influenced by cultivar,fruit harvest maturity and duration of postharvest handling under ambient shelf.展开更多
基金supported by the Deanship of Scientific Research,Vice Presidency for Graduate Studies and Scientific Research,King Faisal University,Saudi Arabia,grant number KFU242733.
文摘The medicinal herb Geranium wallichianum belongs to the family Geraniaceae. The East Asian Himalayas are its primary habitat. Overexploitation and overharvesting pose a threat to this plant, given its extensive ethnomedical utilization in the community. In Pakistan, its population has already declined by over 75%. Given its critical medicinal importance, urgent conservation efforts are needed to prevent extinction. The aim of the current research was to determine the effectiveness of sucrose and polyethylene glycol (PEG) in decelerating the growth of this medicinally important species. Nodal segments were utilized as explant with varying levels of polyethylene glycol (PEG) (0.5% and 1.5%) and sucrose (4% and 8%) as treatments on different growth attributes. Over a 45-day period, PEG treatments (0.5% and 1.5%) proved more efficient compared to sucrose in suppressing in vitro growth, with reduced shoot lengths (0.92 and 0.57 cm), dry weight (0.04 and 0.02 g), and fresh weight (0.06 and 0.04 g). PEG also significantly enhanced phenolic levels (0.96 and 0.19 mg/g) and soluble sugars (4.07 and 4.12 mg/g) while reducing total protein level (2.38 and 2.32 mg/g) and total chlorophyll content (0.17 and 0.14 mg/g). The Pearson correlation analysis showed a negative association between phenolic levels and total chlorophyll content, chlorophyll a, and chlorophyll b. The length of the shoot had a negative relationship with phenolic level but a positive relationship with both fresh and dry weight. The principal component analysis revealed that total sugars and phenolics that were grouped separately from other variables led to stronger responses to osmotic stress. These outcomes suggested that higher PEG was successful in reducing in vitro growth throughout short-term preservation in comparison to sucrose. The outcomes of this research could be applied for the in vitro conservation of medical plants in the future.
文摘Rosa centifolia is grown for its flowers which are used for essential oil extraction. It is vegetatively propagated through its cuttings which are difficult to root. This study was designed to increase the rooting percentage of cuttings by applying plant growth regulators (PGRs) with different levels. In total 31 treatments with different concentrations (450 ppm, 700 ppm and 950 ppm) of indole butyric acid (IBA), indole acetic acid (IAA), naphthalene acetic acid (NAA) alone, in combination and with same concentrations of 6-benzylamino purine (BAP) were used. Healthy similar sized cutting of R. centifolia was treated in solution of PGRs by quick dip method and planted in polythene bags. The data for shoot length, shoot dry weight, number of roots, root length and root dry weight were recorded. All three levels (450 ppm, 700 ppm and 950 ppm) of IBA alone produced maximum results in case of all parameters as compared to all other treatments. Among three levels of IBA, 450 ppm concentration produced maximum shoot length (10.67 cm), shoot dry weight (3.02 g), number of roots (14.00), root length (11.90 cm) and root dry weight (0.50 g). Lower concentrations of plant growth regulators produced better results as compared to higher concentrations.
基金Supporting Project number(RSPD2024R561),King Saud University,Riyadh,Saudi Arabia.
文摘Jujube is an underutilized fruit having high maturity variation at harvest.Investigations were made on eight different cultivars of jujube(Umran,Karela,Gurrh,Amrodia,Mehmood Wali,Aakash,Foladi and Khubani)for changes in antioxidative enzymatic activities,phytochemical functions and proximate composition with respect to different harvest maturity stages(M_(1):mature green,M_(2):yellow and M_(3):crispy brown)under postharvest ambient conditions(25±2℃;60%-65%RH)at three shelf intervals(1,3 and 5 d).Significant genotypic and maturity related variation was recorded regarding studied attributes.The fruit of cv.ʽKhubaniʼhad better attributes among the cultivars evaluated.The crispy brown jujube fruit had higher peroxidase(POD)and catalase(CAT)activity and more protein,total soluble solids(TSS),ash,crude fat and fiber than early mature stages.Over time,the ascorbic acid(vitamin C),titratable acidity(TA),total phenolic(TPC),total antioxidant capacity(TAC),crude protein,crude fiber,moisture,crude fat and activity of antioxidative enzymes[CAT and superoxide dismutase(SOD)]exhibited a significant decline;while increase was recorded in TSS,pH and POD activity.Overall,this work identified that jujube’s antioxidative,biochemical and proximate properties are significantly influenced by cultivar,fruit harvest maturity and duration of postharvest handling under ambient shelf.
