用擎天凤梨短缩茎做外植体培养,通过固液培养对照试验,结果表明:在外植体诱导时,最佳培养基为M S(固)+6 BA 2.0m g.L-1(单位下同)+NAA 0.1;继代增殖培养以M S(液)+6 BA 1.0+IBA 0.1为优,培养30d,其增殖倍数可达5.8;M S(固)+6 BA 0.1+IBA...用擎天凤梨短缩茎做外植体培养,通过固液培养对照试验,结果表明:在外植体诱导时,最佳培养基为M S(固)+6 BA 2.0m g.L-1(单位下同)+NAA 0.1;继代增殖培养以M S(液)+6 BA 1.0+IBA 0.1为优,培养30d,其增殖倍数可达5.8;M S(固)+6 BA 0.1+IBA 3.0为较好的生根培养基。展开更多
Grass carp plays an important role in small-scale aquaculture in Vietnam. However, a severe disease, known in Vietnam as "Red Spot Disease", is causing significant economic loss in grass carp aquaculture. In...Grass carp plays an important role in small-scale aquaculture in Vietnam. However, a severe disease, known in Vietnam as "Red Spot Disease", is causing significant economic loss in grass carp aquaculture. In this study, the tissue samples isolated from the grass carp with Red Spot Disease in Vietnam are investigated and comparied with the control GCHV isolated in China by experimental infection, culture cell infection, serological cross reactivity, and RT-PCR amplification. Infected grass carp exhibits hemorrhage symptoms about 5 days after experimental injection with GCHV-V (Vietnam) strain. The symptoms and lethality induced by the GCHV-V strain are identical to that induced by the Chinese GCHV-9014 strain. The Chinese GCHV-873 strain induces typical cytopathogenic effects in 4 cell lines, such as CIK, CAB, FHM and GCO, from the 6 fish cell lines examined. No cytopathogenic effects are observed in all the 6 examined cell lines, including CAB, FHM, CIK, EPC, CCO and GCO, infected by the GCHV-V strain and GCHV-9014 strain. Immunodiffusion assays demonstrate an obvious cross-reactivity among three GCHV strains. Precipitin lines are clearly observed not only between the anti-GCHV-873 serum and the two strains GCHV-873 and GCHV-9014, but also between the anti-GCHV-873 serum and the GCHV-V strain. GCHV can be detected by immunodiffusion assays after three generations of blind propagations in the cell lines inoculated by GCHV-V strain. This implicates that GCHV-V viruses have been replicated and amplified despite there being no cytopathogenic effects observed in these examined cell lines. Three genome segments of GCHV, including S8, S9 and S10, are amplified by three sets of PCR primers designed according to the segment sequences published recently. The Q8fp and Q8rp primer set specific for genome segment S8 amplifies a 955 bp fragment from the extracted sample of diseased fish with Red Spot Disease, and the fragment size is identical to that amplified by the same primer set from control GCHV-873 strain. Simultaneously, the Q9fp and Q9rp primer set specific for genome segment S9 generates a same 635 bp product, and the Q10fp and Q10rp primer set specific for genome segment S10 produces a same 697 bp fragment from both template samples of diseased fish with Red Spot Disease and control GCHV-873 strain. The RT-PCR amplification and corresponding size comparison data indicate that the three GCHV-V genome segments extracted from the diseased grass carp with Red Spot Disease in Vietnam should be identical to that in control GCHV-873 strain from China. The data confirm that the causative agent of grass carp Red Spot Disease in Vietnam is a virus, and the virus is closely similar to GCHV strain in China.展开更多
The three types of connections (Permanent Connection, Soft Permanent Connection and Switched Connection) provided by ASON can adapt the requirement of different network services. Management and maintenance of these th...The three types of connections (Permanent Connection, Soft Permanent Connection and Switched Connection) provided by ASON can adapt the requirement of different network services. Management and maintenance of these three connections are the most important aspect of ASON management. The information models proposed in this paper are used for the purpose of ASON connection management. Firstly a new information model is proposed to meet the requirement for the control plane introduced by ASON. In this model, a new class ControlNE is given, and the relationship between the ControlNE and the transport NE (network element) is also defined. Then this paper proposes information models for the three types of connections for the first time, and analyzes the relationship between the three kinds of connections and the basic network transport entities. Finally, the paper defines some CORBA interfaces for the management of the three connections. In these interfaces, some operations such as create or release a connection are defined, and some other operations can manage the performance of the three kinds of connections, which is necessary for a distributed management system.展开更多
文摘用擎天凤梨短缩茎做外植体培养,通过固液培养对照试验,结果表明:在外植体诱导时,最佳培养基为M S(固)+6 BA 2.0m g.L-1(单位下同)+NAA 0.1;继代增殖培养以M S(液)+6 BA 1.0+IBA 0.1为优,培养30d,其增殖倍数可达5.8;M S(固)+6 BA 0.1+IBA 3.0为较好的生根培养基。
基金This research was supported by National 863 High Technology Research Foundation of China(2002AA62601)National Natural Science Foundation of China(30170726)+1 种基金the Project of Chinese Academy of Sciences(KSCXZ-SW-302)the Innovation Project of the Institute of Hydrobiology,Chinese Academy of Sciences.
文摘Grass carp plays an important role in small-scale aquaculture in Vietnam. However, a severe disease, known in Vietnam as "Red Spot Disease", is causing significant economic loss in grass carp aquaculture. In this study, the tissue samples isolated from the grass carp with Red Spot Disease in Vietnam are investigated and comparied with the control GCHV isolated in China by experimental infection, culture cell infection, serological cross reactivity, and RT-PCR amplification. Infected grass carp exhibits hemorrhage symptoms about 5 days after experimental injection with GCHV-V (Vietnam) strain. The symptoms and lethality induced by the GCHV-V strain are identical to that induced by the Chinese GCHV-9014 strain. The Chinese GCHV-873 strain induces typical cytopathogenic effects in 4 cell lines, such as CIK, CAB, FHM and GCO, from the 6 fish cell lines examined. No cytopathogenic effects are observed in all the 6 examined cell lines, including CAB, FHM, CIK, EPC, CCO and GCO, infected by the GCHV-V strain and GCHV-9014 strain. Immunodiffusion assays demonstrate an obvious cross-reactivity among three GCHV strains. Precipitin lines are clearly observed not only between the anti-GCHV-873 serum and the two strains GCHV-873 and GCHV-9014, but also between the anti-GCHV-873 serum and the GCHV-V strain. GCHV can be detected by immunodiffusion assays after three generations of blind propagations in the cell lines inoculated by GCHV-V strain. This implicates that GCHV-V viruses have been replicated and amplified despite there being no cytopathogenic effects observed in these examined cell lines. Three genome segments of GCHV, including S8, S9 and S10, are amplified by three sets of PCR primers designed according to the segment sequences published recently. The Q8fp and Q8rp primer set specific for genome segment S8 amplifies a 955 bp fragment from the extracted sample of diseased fish with Red Spot Disease, and the fragment size is identical to that amplified by the same primer set from control GCHV-873 strain. Simultaneously, the Q9fp and Q9rp primer set specific for genome segment S9 generates a same 635 bp product, and the Q10fp and Q10rp primer set specific for genome segment S10 produces a same 697 bp fragment from both template samples of diseased fish with Red Spot Disease and control GCHV-873 strain. The RT-PCR amplification and corresponding size comparison data indicate that the three GCHV-V genome segments extracted from the diseased grass carp with Red Spot Disease in Vietnam should be identical to that in control GCHV-873 strain from China. The data confirm that the causative agent of grass carp Red Spot Disease in Vietnam is a virus, and the virus is closely similar to GCHV strain in China.
文摘The three types of connections (Permanent Connection, Soft Permanent Connection and Switched Connection) provided by ASON can adapt the requirement of different network services. Management and maintenance of these three connections are the most important aspect of ASON management. The information models proposed in this paper are used for the purpose of ASON connection management. Firstly a new information model is proposed to meet the requirement for the control plane introduced by ASON. In this model, a new class ControlNE is given, and the relationship between the ControlNE and the transport NE (network element) is also defined. Then this paper proposes information models for the three types of connections for the first time, and analyzes the relationship between the three kinds of connections and the basic network transport entities. Finally, the paper defines some CORBA interfaces for the management of the three connections. In these interfaces, some operations such as create or release a connection are defined, and some other operations can manage the performance of the three kinds of connections, which is necessary for a distributed management system.
