Objectives:Radiation enteropathy is a common complication of cervical cancer radiotherapy,and there is currently no effective treatment.This study evaluates the radioprotective effect of Premna microphylla Turcz.(PMT)...Objectives:Radiation enteropathy is a common complication of cervical cancer radiotherapy,and there is currently no effective treatment.This study evaluates the radioprotective effect of Premna microphylla Turcz.(PMT)on radiation-induced colon injury in mice.Materials and Methods:An 8 Gy abdominal irradiation model was established in mice,which were then gavaged with low(0.325 g/kg),medium(0.65 g/kg),or high(1.3 g/kg)doses of PMT.Weight and other routine information were recorded.The mice were euthanized on the 7 th day after radiotherapy.Colonic tissue was collected for observing structural changes.Reverse transcription quantitative polymerase chain reaction(RTqPCR)was used to detect inflammation infiltration in colon tissue.16S rRNA gene sequencing was performed to analyze the alterations in gut microbiota composition.Hepatic and renal function assessments were conducted to evaluate the safety profile of PMT.Results:PMT at medium dose significantly reduced radiation-induced weight loss(P<0.05).PMT alleviated gastrointestinal discomfort and partially reduced radiotherapy-induced faecal occult blood,although these effects were not statistically significant.Both medium and high doses of PMT reversed the shortening of colon length caused by radiotherapy(both P<0.05).The medium-and high-dose PMT improved the colonic inflammatory microenvironment by reducing the expression of inflammatory factors and chemokines(IL-1β,IL-6,IFN-γ,TNF-α,and Cxcl1)(P<0.05).PMT restored the diversity of the gut microbiota and normalized the Bacteroidetes/Firmicutes ratio disrupted by radiation.Beyond intestinal protection,PMT administration at all doses significantly reduced the postirradiation levels of serum creatinine(Cr),alanine aminotransferase(ALT),and aspartate aminotransferase(AST)(all P<0.05).Conclusions:PMT,particularly at medium doses,has potential for treating radiation-induced colonic injury in mice without causing hepatorenal toxicity,providing a novel therapeutic approach for radiation-induced colonic diseases.展开更多
Immunotherapies have demonstrated notable clinical benefits in the treatment of cervical cancer(CC).However,the development of therapeutic resistance and diverse adverse effects in immunotherapy stem from complex inte...Immunotherapies have demonstrated notable clinical benefits in the treatment of cervical cancer(CC).However,the development of therapeutic resistance and diverse adverse effects in immunotherapy stem from complex interactions among biological processes and factors within the tumor immune microenvironment(TIME).Advanced omic technologies offer novel insights into a more expansive and thorough layer of the TIME.Furthermore,integrating multidimensional omics within the frameworks of systems biology and computational methodologies facilitates the generation of interpretable data outputs to characterize the clinical and biological trajectories of tumor behavior.In this review,we present advanced omics technologies that utilize various clinical samples to address scientific inquiries related to immunotherapies for CC,highlighting their utility in identifying metastasis dissemination,recurrence risk,and therapeutic resistance in patients treated with immunotherapeutic approaches.This review elaborates on the strategy for integrating multi-omics data through artificial intelligence algorithms.Additionally,an analysis of the obstacles encountered in the multi-omics analysis process and potential avenues for future research in this domain are presented.展开更多
基金supported by the Zhejiang Province Traditional Chinese Medicine Key Laboratory Project(No.GZY-ZJ-SY-2303)the Cultivation Foundation of National Natural Science Foundation of Zhejiang Cancer Hospital(No.PY2024047)+1 种基金the Joint TCM Science&Technology Projects of National Demonstration Zones for Comprehensive TCM Reform(No.GZY-KJS-ZJ-2025-036)the Key Project of Zhejiang Provincial Administration of Traditional Chinese Medicine(No.GZY-ZJ-KJ-23050),China。
文摘Objectives:Radiation enteropathy is a common complication of cervical cancer radiotherapy,and there is currently no effective treatment.This study evaluates the radioprotective effect of Premna microphylla Turcz.(PMT)on radiation-induced colon injury in mice.Materials and Methods:An 8 Gy abdominal irradiation model was established in mice,which were then gavaged with low(0.325 g/kg),medium(0.65 g/kg),or high(1.3 g/kg)doses of PMT.Weight and other routine information were recorded.The mice were euthanized on the 7 th day after radiotherapy.Colonic tissue was collected for observing structural changes.Reverse transcription quantitative polymerase chain reaction(RTqPCR)was used to detect inflammation infiltration in colon tissue.16S rRNA gene sequencing was performed to analyze the alterations in gut microbiota composition.Hepatic and renal function assessments were conducted to evaluate the safety profile of PMT.Results:PMT at medium dose significantly reduced radiation-induced weight loss(P<0.05).PMT alleviated gastrointestinal discomfort and partially reduced radiotherapy-induced faecal occult blood,although these effects were not statistically significant.Both medium and high doses of PMT reversed the shortening of colon length caused by radiotherapy(both P<0.05).The medium-and high-dose PMT improved the colonic inflammatory microenvironment by reducing the expression of inflammatory factors and chemokines(IL-1β,IL-6,IFN-γ,TNF-α,and Cxcl1)(P<0.05).PMT restored the diversity of the gut microbiota and normalized the Bacteroidetes/Firmicutes ratio disrupted by radiation.Beyond intestinal protection,PMT administration at all doses significantly reduced the postirradiation levels of serum creatinine(Cr),alanine aminotransferase(ALT),and aspartate aminotransferase(AST)(all P<0.05).Conclusions:PMT,particularly at medium doses,has potential for treating radiation-induced colonic injury in mice without causing hepatorenal toxicity,providing a novel therapeutic approach for radiation-induced colonic diseases.
基金supported by the Zhejiang Province Traditional Chinese Medicine Science and Technology Project(GZY-ZJ-KJ-24063)the Natural Science Foundation of Zhejiang Province(Q24H290031)the Key Laboratory for Molecular Medicine and Chinese Medicine Preparations(No.GZY-ZJ-SY-2303).
文摘Immunotherapies have demonstrated notable clinical benefits in the treatment of cervical cancer(CC).However,the development of therapeutic resistance and diverse adverse effects in immunotherapy stem from complex interactions among biological processes and factors within the tumor immune microenvironment(TIME).Advanced omic technologies offer novel insights into a more expansive and thorough layer of the TIME.Furthermore,integrating multidimensional omics within the frameworks of systems biology and computational methodologies facilitates the generation of interpretable data outputs to characterize the clinical and biological trajectories of tumor behavior.In this review,we present advanced omics technologies that utilize various clinical samples to address scientific inquiries related to immunotherapies for CC,highlighting their utility in identifying metastasis dissemination,recurrence risk,and therapeutic resistance in patients treated with immunotherapeutic approaches.This review elaborates on the strategy for integrating multi-omics data through artificial intelligence algorithms.Additionally,an analysis of the obstacles encountered in the multi-omics analysis process and potential avenues for future research in this domain are presented.
