随着清洁能源规模的不断扩大,可平滑电能输出的混合储能系统(hybrid energy storage system,HESS)逐渐受到广泛关注。双有源桥可实现电气隔离和软开关,常应用于HESS中。为拓宽电压增益范围并降低储能装置侧电流纹波,文中提出一种基于电...随着清洁能源规模的不断扩大,可平滑电能输出的混合储能系统(hybrid energy storage system,HESS)逐渐受到广泛关注。双有源桥可实现电气隔离和软开关,常应用于HESS中。为拓宽电压增益范围并降低储能装置侧电流纹波,文中提出一种基于电流源型双向谐振变换器的HESS拓扑。首先,给出变换器的拓扑及等效电路,分析其开关模态和工作原理,推导出等效电路模型、电压增益表达式及低压侧电流纹波特性。在此基础上,提出一种解耦控制策略,通过调节各储能装置侧全桥的占空比独立控制超级电容和蓄电池的传输功率,并根据功率分配指令动态调整各端口功率占比。仿真结果表明,所提系统能实现各开关管的零电压开通(zero voltage switching,ZVS),在负载切换和功率指令变化时均表现出快速的动态响应和良好的稳定性。展开更多
OBJECTIVE:To observe the efficacy of Danggui Buxue decoction(当归补血汤,DBD) on diabetic nephropathyinduced renal fibrosis in rats,and to study the possible mechanism.METHODS:Sixty male Goto Kakizaki(GK) rats were ran...OBJECTIVE:To observe the efficacy of Danggui Buxue decoction(当归补血汤,DBD) on diabetic nephropathyinduced renal fibrosis in rats,and to study the possible mechanism.METHODS:Sixty male Goto Kakizaki(GK) rats were randomly assigned to the model group,gliquidone group,astragaloside Ⅳ group,and high-,medium-and lowdoses DBD groups.After 8 weeks,changes in body weight,blood glucose,serum creatinine,serum urea nitrogen,and total cholesterol were observed.Changes in transforming growth factor-β1(TGF-β1),Smad3,and Smad5 pathways and the expression of the fibrosisrelated proteins collagen Ⅳ(col Ⅳ),α-smooth muscle actin(α-SMA),and vimentin were assessed.The degree of renal fibrosis was observed by immunohistochemistry and Mason staining.The expression of interleukin 6(IL-6),interleukin 10(IL-10),tumor necrosis factor(TNF-α),and C-reactive protein(CRP) in the kidneys was assessed using enzyme linked immunosorbent assay.RESULTS:Our experiments showed that DBD effectively reduced blood glucose,blood urea nitrogen,and creatinine levels after 8 weeks of administration,improved renal function in diabetic rats,alleviated renal fibrosis,and reduced the renal tissue levels of IL-6,IL-10,TNF-α,and CRP.Furthermore,DBD decreased the expression of TGF-β1,Smad3,col IV,α-SMA,and vimentin in renal tissues and increased the expression of Smad5.CONCLUSIONS:DBD ameliorates diabetic renal interstitial fibrosis by modulating the TGF-β1/Smads pathway.展开更多
Objective To explore the role of glucocorticoid （GC） receptor （GR） in rapamycin＇s reversion of GC resistance in humanGC-resistant T-acute lymphoblastic leukemia （ALL） CEM-C1 cells. Methods CEM-C1 cells were cul...Objective To explore the role of glucocorticoid （GC） receptor （GR） in rapamycin＇s reversion of GC resistance in humanGC-resistant T-acute lymphoblastic leukemia （ALL） CEM-C1 cells. Methods CEM-C1 cells were cultured in vitro and treated with rapamycin at different concentrations with or without 1 μmol/L dexamethasone （Dex）. 3-（4, 5-dimethylthiazol-2-yl）-2, 5-diphenyltetrazolium bromide （MTT） test was performed to assess cell proliferation. The cell cycle and cell apoptosis were analyzed by flow cytometry. The expression of GRα mRNA was determined by real-time quantitative RT-PCR. The expression of GR, p-70S6K, Mcl-1, and Bim proteins was detected by Western blot. Results When incubated with rapamycin at different concentrations, CEM-C1 cells showed significant growth inhibition in a time- and concentration-dependent manner. The growth inhibition was synergistically increased when CEM-C1 cells were treated with rapamycin plus 1 μmol/L Dex. CEM-C1 cells treated with rapamycin alone showed no apparent apoptosis, and were arrested at G0/G1 phase. After the treatment with Dex plus rapamycin, CEM-C1 cells demonstrated apparent apoptosis and increased the cell cycle arrested at G0/G1 phase. Rapamycin combined with Dex up-regulated GRα, phosphorylated GR（p-GR）, and pro-apoptotic protein Bim-EL in CEM-C1 cells, but inhibited the expression of p-p70S6K, a downstream target protein ofmTOR （mammalian target of rapamycin）. Conclusion After the treatment with rapamycin plus Dex, Dex resistant CEM-C1 cells induce growth inhibition and apoptosis. The underlying mechanism may involve inhibition of the mTOR signaling pathway and also be associated with up-regulation of GR expression and activation of GC-GR signaling pathway.展开更多
三相双有源桥双向AC/DC变换器具备高效双向变换和升降压运行的优势,适合应用于储能系统,但是该变换器在宽电压范围下运行时的参数设计与性能优化面临挑战。针对适用于该变换器在宽电压范围下实现零电压开关(zero voltage switching,ZVS...三相双有源桥双向AC/DC变换器具备高效双向变换和升降压运行的优势,适合应用于储能系统,但是该变换器在宽电压范围下运行时的参数设计与性能优化面临挑战。针对适用于该变换器在宽电压范围下实现零电压开关(zero voltage switching,ZVS)的混合调制策略,提出基于遗传算法的多目标参数优化方法;以开关管损耗和电感电流有效值作为优化目标,建立多目标优化模型,对电感值和变压器匝比进行求解;在实验室搭建一台1.5 kW的原理样机,实验结果表明,采用混合调制策略的变换器可在宽电压和宽功率范围下实现ZVS。此外,多目标优化参数设计方法能降低变换器的损耗,从而提升效率,为其性能提升提供了有效方案。展开更多
The internal solitary wave(ISW)represents a frequent and severe oceanic dynamic phenomenon observed in the South China Sea,exposing marine structures to sudden loads.This paper examines the prediction model of interac...The internal solitary wave(ISW)represents a frequent and severe oceanic dynamic phenomenon observed in the South China Sea,exposing marine structures to sudden loads.This paper examines the prediction model of interaction loads between ISW and FPSO,accounting for varying attack angles and incorporating ISW theories.The research demonstrates that the horizontal and transverse forces on FPSO under internal solitary waves(ISWs)comprise wave pressure difference force and viscous force,while the vertical force primarily consists of vertical wave pressure difference force.The wave pressure difference force is determined using the Froude-Krylov equation.The viscous force is derived from the tangential particle velocity induced by ISW and the viscous coefficient.The viscous coefficient formula is obtained through regression analysis of experimental data with different ISW attack angles.The research reveals that the horizontal viscous coefficient C_(vx)decreases as Reynolds number(R_(e))increases,while the transverse viscous coefficient C_(vy)initially increases and subsequently decreases with the growth of the Keulegan-Carpenter number(KC).Moreover,changes in wave propagation direction significantly affect the extreme magnitudes of both horizontal and transverse forces,and simultaneously modify the transverse force orientation,while having minimal impact on the vertical force.Additionally,the forces increase with the ISW’s amplitude.For horizontal and transverse forces,a thinner upper fluid layer generates larger forces.Comparative analysis of experimental,numerical,and theoretical results indicates strong agreement between theoretical predictions and experimental and numerical outcomes.展开更多
文摘随着清洁能源规模的不断扩大,可平滑电能输出的混合储能系统(hybrid energy storage system,HESS)逐渐受到广泛关注。双有源桥可实现电气隔离和软开关,常应用于HESS中。为拓宽电压增益范围并降低储能装置侧电流纹波,文中提出一种基于电流源型双向谐振变换器的HESS拓扑。首先,给出变换器的拓扑及等效电路,分析其开关模态和工作原理,推导出等效电路模型、电压增益表达式及低压侧电流纹波特性。在此基础上,提出一种解耦控制策略,通过调节各储能装置侧全桥的占空比独立控制超级电容和蓄电池的传输功率,并根据功率分配指令动态调整各端口功率占比。仿真结果表明,所提系统能实现各开关管的零电压开通(zero voltage switching,ZVS),在负载切换和功率指令变化时均表现出快速的动态响应和良好的稳定性。
基金Supported by National Natural Science Foundation of China Youth Fund:Research on the Mechanism of Danggui Buxue Decoction in the Treatment of Diabetic Renal Interstitial Fibrosis Based on the Regulation of TGF-β1/Smad3 Signaling Pathway by Mi RNA-27a(No.81904085)Nanjing University of Chinese Medicine Foundation Youth Project:Research on the Mechanism of Danggui Buxue Decoction in the Treatment of Diabetic Renal Interstitial Fibrosis Based on the Regulation of TGF-β1/Smad3 Signaling Pathway by Mi RNA-27a(No.NZY81904085)。
文摘OBJECTIVE:To observe the efficacy of Danggui Buxue decoction(当归补血汤,DBD) on diabetic nephropathyinduced renal fibrosis in rats,and to study the possible mechanism.METHODS:Sixty male Goto Kakizaki(GK) rats were randomly assigned to the model group,gliquidone group,astragaloside Ⅳ group,and high-,medium-and lowdoses DBD groups.After 8 weeks,changes in body weight,blood glucose,serum creatinine,serum urea nitrogen,and total cholesterol were observed.Changes in transforming growth factor-β1(TGF-β1),Smad3,and Smad5 pathways and the expression of the fibrosisrelated proteins collagen Ⅳ(col Ⅳ),α-smooth muscle actin(α-SMA),and vimentin were assessed.The degree of renal fibrosis was observed by immunohistochemistry and Mason staining.The expression of interleukin 6(IL-6),interleukin 10(IL-10),tumor necrosis factor(TNF-α),and C-reactive protein(CRP) in the kidneys was assessed using enzyme linked immunosorbent assay.RESULTS:Our experiments showed that DBD effectively reduced blood glucose,blood urea nitrogen,and creatinine levels after 8 weeks of administration,improved renal function in diabetic rats,alleviated renal fibrosis,and reduced the renal tissue levels of IL-6,IL-10,TNF-α,and CRP.Furthermore,DBD decreased the expression of TGF-β1,Smad3,col IV,α-SMA,and vimentin in renal tissues and increased the expression of Smad5.CONCLUSIONS:DBD ameliorates diabetic renal interstitial fibrosis by modulating the TGF-β1/Smads pathway.
基金supported by the research funds from the University Program for Changjiang Scholars and Innovative Research Team(No.IRT0935)the National Natural Science Fund Project(No.30973237)grants from the Department of Science and Technology of Sichuan Province(No.2008JY0029-1,No.07FG002-024,and No.2010JY0004)
文摘Objective To explore the role of glucocorticoid （GC） receptor （GR） in rapamycin＇s reversion of GC resistance in humanGC-resistant T-acute lymphoblastic leukemia （ALL） CEM-C1 cells. Methods CEM-C1 cells were cultured in vitro and treated with rapamycin at different concentrations with or without 1 μmol/L dexamethasone （Dex）. 3-（4, 5-dimethylthiazol-2-yl）-2, 5-diphenyltetrazolium bromide （MTT） test was performed to assess cell proliferation. The cell cycle and cell apoptosis were analyzed by flow cytometry. The expression of GRα mRNA was determined by real-time quantitative RT-PCR. The expression of GR, p-70S6K, Mcl-1, and Bim proteins was detected by Western blot. Results When incubated with rapamycin at different concentrations, CEM-C1 cells showed significant growth inhibition in a time- and concentration-dependent manner. The growth inhibition was synergistically increased when CEM-C1 cells were treated with rapamycin plus 1 μmol/L Dex. CEM-C1 cells treated with rapamycin alone showed no apparent apoptosis, and were arrested at G0/G1 phase. After the treatment with Dex plus rapamycin, CEM-C1 cells demonstrated apparent apoptosis and increased the cell cycle arrested at G0/G1 phase. Rapamycin combined with Dex up-regulated GRα, phosphorylated GR（p-GR）, and pro-apoptotic protein Bim-EL in CEM-C1 cells, but inhibited the expression of p-p70S6K, a downstream target protein ofmTOR （mammalian target of rapamycin）. Conclusion After the treatment with rapamycin plus Dex, Dex resistant CEM-C1 cells induce growth inhibition and apoptosis. The underlying mechanism may involve inhibition of the mTOR signaling pathway and also be associated with up-regulation of GR expression and activation of GC-GR signaling pathway.
文摘三相双有源桥双向AC/DC变换器具备高效双向变换和升降压运行的优势,适合应用于储能系统,但是该变换器在宽电压范围下运行时的参数设计与性能优化面临挑战。针对适用于该变换器在宽电压范围下实现零电压开关(zero voltage switching,ZVS)的混合调制策略,提出基于遗传算法的多目标参数优化方法;以开关管损耗和电感电流有效值作为优化目标,建立多目标优化模型,对电感值和变压器匝比进行求解;在实验室搭建一台1.5 kW的原理样机,实验结果表明,采用混合调制策略的变换器可在宽电压和宽功率范围下实现ZVS。此外,多目标优化参数设计方法能降低变换器的损耗,从而提升效率,为其性能提升提供了有效方案。
基金supported by JUST Start-up Fund for Science Research,the Jiangsu Natural Science Foundation(Grant No.BK20210885).
文摘The internal solitary wave(ISW)represents a frequent and severe oceanic dynamic phenomenon observed in the South China Sea,exposing marine structures to sudden loads.This paper examines the prediction model of interaction loads between ISW and FPSO,accounting for varying attack angles and incorporating ISW theories.The research demonstrates that the horizontal and transverse forces on FPSO under internal solitary waves(ISWs)comprise wave pressure difference force and viscous force,while the vertical force primarily consists of vertical wave pressure difference force.The wave pressure difference force is determined using the Froude-Krylov equation.The viscous force is derived from the tangential particle velocity induced by ISW and the viscous coefficient.The viscous coefficient formula is obtained through regression analysis of experimental data with different ISW attack angles.The research reveals that the horizontal viscous coefficient C_(vx)decreases as Reynolds number(R_(e))increases,while the transverse viscous coefficient C_(vy)initially increases and subsequently decreases with the growth of the Keulegan-Carpenter number(KC).Moreover,changes in wave propagation direction significantly affect the extreme magnitudes of both horizontal and transverse forces,and simultaneously modify the transverse force orientation,while having minimal impact on the vertical force.Additionally,the forces increase with the ISW’s amplitude.For horizontal and transverse forces,a thinner upper fluid layer generates larger forces.Comparative analysis of experimental,numerical,and theoretical results indicates strong agreement between theoretical predictions and experimental and numerical outcomes.
