In search of a powerful source of bioactive molecules that can be imperative in maintaining health,this paper studies two Corydalis species from the territory of Turkey.Namely,C.erdelii Zucc.and C.solida(L.)Clairv.wer...In search of a powerful source of bioactive molecules that can be imperative in maintaining health,this paper studies two Corydalis species from the territory of Turkey.Namely,C.erdelii Zucc.and C.solida(L.)Clairv.were extracted with ethanol using homogenized-assisted extraction,and infusions of these two plant species were obtained using water.The obtained extracts and infusions were characterized in terms of chemical composition,namely in terms of the content of total phenols and flavonoids,as well as individual polyphenolic components using the LC-method of analysis.Along with the examination of the chemical composition,their biological ac-tivity was also determined using a series of tests that defined their antioxidant,antidiabetic,neuroprotective and cytotoxic activity.The obtained results showed that infusion of C.solida was richer with biomolecules capable to neutralize DPPH,ABTS radicals but also with better reduction power.Ethanolic extracts also showed a higher degree of inhibition of the observed enzymes.Treatment of A549 lung cancer cells with Corydalis extracts,especially ethanol extracts,triggered up-regulation of apoptotic and ER stress pathway genes,potentially attributed to compounds like quercetin and verbascoside.C.erdelii demonstrated superior apoptotic activity compared to C.solida,possibly due to unique compounds.In BEAS-2B cells,Corydalis extracts induced apoptosis while suppressing ER stress,suggesting a mechanism primarily driven by apoptosis.The molecular docking study identified eight compounds from Corydalis extracts with strong inhibitory activity against enzymes.Compounds like kaempferol 3-O-rhamnoside,rhamnocitrin 3-rhamnoside,and verbascoside showed stable interactions with proteins,suggesting their potential for further drug development.展开更多
Flueggea virosa (Roxb. ex Willd.) Royle is a plant with several traditional uses in tropical countries. To study the potential usefulness, extracts from stem barks and leaves of F. virosa were obtained with different ...Flueggea virosa (Roxb. ex Willd.) Royle is a plant with several traditional uses in tropical countries. To study the potential usefulness, extracts from stem barks and leaves of F. virosa were obtained with different solvents. The phytochemical composition was assessed using LC-MS and NMR. Triterpenes, as oleanolic (0.05-88.09 mg/g extract), and ursolic (0.03-94.36 mg/g extract) acids were determined. In addition, ellagic and gallic acid derivatives were present in large concentrations in some of the extracts. Antioxidant (radical scavenging, reduction ability, and metal chelating) and enzyme inhibitory (cholinesterase, tyrosinase, amylase, and glucosidase) effects were assessed by in vitro chemical assays. The methanol extracts and infusions from both parts show higher antioxidant ability when compared with ethyl acetate extracts, and the antioxidant capacity was correlated with the total phenolic contents of the tested extracts. The extracts demonstrate enzyme inhibitory abilities on AChE and BChE, and multivariate approaches allowed for correlating the activities with the presence of triterpenoids (R > 0.8). Furthermore, molecular docking was used to get insights into the interactions between the triterpenoids and the tested enzymes. Together, these results indicate that F. virosa represents a significant source of naturally-occurring bioactive compounds that can be exploited as a new ingredient for the development of novel functional products with promising biological properties.展开更多
文摘In search of a powerful source of bioactive molecules that can be imperative in maintaining health,this paper studies two Corydalis species from the territory of Turkey.Namely,C.erdelii Zucc.and C.solida(L.)Clairv.were extracted with ethanol using homogenized-assisted extraction,and infusions of these two plant species were obtained using water.The obtained extracts and infusions were characterized in terms of chemical composition,namely in terms of the content of total phenols and flavonoids,as well as individual polyphenolic components using the LC-method of analysis.Along with the examination of the chemical composition,their biological ac-tivity was also determined using a series of tests that defined their antioxidant,antidiabetic,neuroprotective and cytotoxic activity.The obtained results showed that infusion of C.solida was richer with biomolecules capable to neutralize DPPH,ABTS radicals but also with better reduction power.Ethanolic extracts also showed a higher degree of inhibition of the observed enzymes.Treatment of A549 lung cancer cells with Corydalis extracts,especially ethanol extracts,triggered up-regulation of apoptotic and ER stress pathway genes,potentially attributed to compounds like quercetin and verbascoside.C.erdelii demonstrated superior apoptotic activity compared to C.solida,possibly due to unique compounds.In BEAS-2B cells,Corydalis extracts induced apoptosis while suppressing ER stress,suggesting a mechanism primarily driven by apoptosis.The molecular docking study identified eight compounds from Corydalis extracts with strong inhibitory activity against enzymes.Compounds like kaempferol 3-O-rhamnoside,rhamnocitrin 3-rhamnoside,and verbascoside showed stable interactions with proteins,suggesting their potential for further drug development.
文摘Flueggea virosa (Roxb. ex Willd.) Royle is a plant with several traditional uses in tropical countries. To study the potential usefulness, extracts from stem barks and leaves of F. virosa were obtained with different solvents. The phytochemical composition was assessed using LC-MS and NMR. Triterpenes, as oleanolic (0.05-88.09 mg/g extract), and ursolic (0.03-94.36 mg/g extract) acids were determined. In addition, ellagic and gallic acid derivatives were present in large concentrations in some of the extracts. Antioxidant (radical scavenging, reduction ability, and metal chelating) and enzyme inhibitory (cholinesterase, tyrosinase, amylase, and glucosidase) effects were assessed by in vitro chemical assays. The methanol extracts and infusions from both parts show higher antioxidant ability when compared with ethyl acetate extracts, and the antioxidant capacity was correlated with the total phenolic contents of the tested extracts. The extracts demonstrate enzyme inhibitory abilities on AChE and BChE, and multivariate approaches allowed for correlating the activities with the presence of triterpenoids (R > 0.8). Furthermore, molecular docking was used to get insights into the interactions between the triterpenoids and the tested enzymes. Together, these results indicate that F. virosa represents a significant source of naturally-occurring bioactive compounds that can be exploited as a new ingredient for the development of novel functional products with promising biological properties.
