Prebiotics,indigestible oligosaccharides found primarily in plant bi-product,aided probiotic development.Banana peels were used as cereal by-products in this study.The possibility of using them as an antistress prebio...Prebiotics,indigestible oligosaccharides found primarily in plant bi-product,aided probiotic development.Banana peels were used as cereal by-products in this study.The possibility of using them as an antistress prebiotics efficacy was examined through in-vitro and in-vivo analysis.Fructo-oligosaccharides(1-kestose(GF2))in Bannan peel oligosaccharide enzymatic extract(BPOE)were purified,further prebiotic effectiveness was analyzed and the anti-stress effect was compared in mice.In addition,Caenorhabditis elegans and fibroblasts cell line on prebiotic efficacy and cytotoxicity was determined.As a result of enzymatic treatment,kestose(GF2)was quantified using HPLC-RID,HSCC Chromatography at 24.9 mg/g,and COZY confirmed the structural.Further,the oligosaccharide enriched cookies enhanced the growth of Lactobacillus rhamnosus.Additionally,the in-vivo result indicates that the kestose fed mice reduced the stress and significantly decreased corticosterone levels,mainly due to changes in microbiota,such as an increase in the abundance of Oscilospira,Mucisprillum,and Parabactoroides species likewise decrease in Clostridium,coprobacillus,and Sporosarona species,correspondingly the behaviour change was confirmed for anxiolytic effect.This framework has the potential to advance the treatment of clinical anxiety.展开更多
基金The National Research Foundation of Korea funded this research(NRF)grant number 2018007551Brain Korea(BK)21 Plus Project[Grant No.22A20153713433]+1 种基金Korea Research Fellowship(KRF)Program(Grant No:2020H1D3A1A02081423)through the National Research Foundation of Korea(NRF)funded by the Ministry of Science and ICT,Republic of Korea and Department of Food Science and Biotechnology,Kangwon Institute of Inclusive Technology(KIIT)Kangwon National University,Gangwon-do 24341,Korea.
文摘Prebiotics,indigestible oligosaccharides found primarily in plant bi-product,aided probiotic development.Banana peels were used as cereal by-products in this study.The possibility of using them as an antistress prebiotics efficacy was examined through in-vitro and in-vivo analysis.Fructo-oligosaccharides(1-kestose(GF2))in Bannan peel oligosaccharide enzymatic extract(BPOE)were purified,further prebiotic effectiveness was analyzed and the anti-stress effect was compared in mice.In addition,Caenorhabditis elegans and fibroblasts cell line on prebiotic efficacy and cytotoxicity was determined.As a result of enzymatic treatment,kestose(GF2)was quantified using HPLC-RID,HSCC Chromatography at 24.9 mg/g,and COZY confirmed the structural.Further,the oligosaccharide enriched cookies enhanced the growth of Lactobacillus rhamnosus.Additionally,the in-vivo result indicates that the kestose fed mice reduced the stress and significantly decreased corticosterone levels,mainly due to changes in microbiota,such as an increase in the abundance of Oscilospira,Mucisprillum,and Parabactoroides species likewise decrease in Clostridium,coprobacillus,and Sporosarona species,correspondingly the behaviour change was confirmed for anxiolytic effect.This framework has the potential to advance the treatment of clinical anxiety.
