Transparent microelectrode arrays enable simultaneous electrical recording and optical imaging of neuronal networks in the brain.Electrodes made of the conducting polymer poly(3,4-ethylenedioxythiophene)doped with pol...Transparent microelectrode arrays enable simultaneous electrical recording and optical imaging of neuronal networks in the brain.Electrodes made of the conducting polymer poly(3,4-ethylenedioxythiophene)doped with polystyrene sulfonate(PEDOT:PSS)are transparent;however,device fabrication necessitates specific processes to avoid deterioration of the organic material.Here,we present an innovative fabrication scheme for a neural probe that consists of transparent PEDOT:PSS electrodes and demonstrate its compatibility with 2-photon microscopy.The electrodes show suitable impedance to record local field potentials from the cortex of mice and sufficient transparency to visualize GCaMP6f-expressing neurons underneath the PEDOT:PSS features.The results validate the performance of the neural probe,which paves the way to study the complex dynamics of in vivo neuronal activity with both a high spatial and temporal resolution to better understand the brain.展开更多
Glioblastoma is a highly aggressive brain tumor,very invasive and thus difficult to eradicate with standard oncology therapies.Bioelectric treatments based on pulsed electric fields have proven to be a successful meth...Glioblastoma is a highly aggressive brain tumor,very invasive and thus difficult to eradicate with standard oncology therapies.Bioelectric treatments based on pulsed electric fields have proven to be a successful method to treat cancerous tissues.However,they rely on stiff electrodes,which cause acute and chronic injuries,especially in soft tissues like the brain.Here we demonstrate the feasibility of delivering pulsed electric fields with flexible electronics using an in ovo vascularized tumor model.We show with fluorescence widefield and multiphoton microscopy that pulsed electric fields induce vasoconstriction of blood vessels and evoke calcium signals in vascularized glioblastoma spheroids stably expressing a genetically encoded fluorescence reporter.Simulations of the electric field delivery are compared with the measured influence of electric field effects on cell membrane integrity in exposed tumor cells.Our results confirm the feasibility of flexible electronics as a means of delivering intense pulsed electric fields to tumors in an intravital 3D vascularized model of human glioblastoma.展开更多
文摘Transparent microelectrode arrays enable simultaneous electrical recording and optical imaging of neuronal networks in the brain.Electrodes made of the conducting polymer poly(3,4-ethylenedioxythiophene)doped with polystyrene sulfonate(PEDOT:PSS)are transparent;however,device fabrication necessitates specific processes to avoid deterioration of the organic material.Here,we present an innovative fabrication scheme for a neural probe that consists of transparent PEDOT:PSS electrodes and demonstrate its compatibility with 2-photon microscopy.The electrodes show suitable impedance to record local field potentials from the cortex of mice and sufficient transparency to visualize GCaMP6f-expressing neurons underneath the PEDOT:PSS features.The results validate the performance of the neural probe,which paves the way to study the complex dynamics of in vivo neuronal activity with both a high spatial and temporal resolution to better understand the brain.
基金supported by the French National Research Agency(ANR-18-CE19-0029).
文摘Glioblastoma is a highly aggressive brain tumor,very invasive and thus difficult to eradicate with standard oncology therapies.Bioelectric treatments based on pulsed electric fields have proven to be a successful method to treat cancerous tissues.However,they rely on stiff electrodes,which cause acute and chronic injuries,especially in soft tissues like the brain.Here we demonstrate the feasibility of delivering pulsed electric fields with flexible electronics using an in ovo vascularized tumor model.We show with fluorescence widefield and multiphoton microscopy that pulsed electric fields induce vasoconstriction of blood vessels and evoke calcium signals in vascularized glioblastoma spheroids stably expressing a genetically encoded fluorescence reporter.Simulations of the electric field delivery are compared with the measured influence of electric field effects on cell membrane integrity in exposed tumor cells.Our results confirm the feasibility of flexible electronics as a means of delivering intense pulsed electric fields to tumors in an intravital 3D vascularized model of human glioblastoma.
