Various approaches have been proposed for point-of-care diagnostics, and in particular, optical detection is preferred because it is relatively simple and fast. At the same time, field-effect transistor （FET）-based ...Various approaches have been proposed for point-of-care diagnostics, and in particular, optical detection is preferred because it is relatively simple and fast. At the same time, field-effect transistor （FET）-based biosensors have attracted great attention because they can provide highly sensitive and label-free detection. In this work we present highly sensitive, epidermal skin-type point-of-care devices with system-level integration of flexible MOS2 FET biosensors, read-out circuits, and light-emitting diode （LEDs） that enable real-time detection of prostate cancer antigens （PSA）. Regardless of the physical forms or mechanical stress conditions, our proposed high-performance MoS2 biosensors can detect a PSA concentration of 1 pg.mL-1 without specific surface treatment for anti-PSA immobilization on the MoS2 surface on which we characterize and confirm physisorption of anti-PSA using Kelvin probe force microscopy （KPFM） and tapping-mode atomic force microscopy （tm-AFM）. Furthermore, current modulation induced by the binding process was stably maintained for longer than 2-3 min. The results indicate that flexible MoS2-based FET biosensors have great potential for point-of-care diagnostics for prostate cancer as well as other biomarkers.展开更多
Precise diagnosis and immunity to viruses,such as severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)and Middle East respiratory syndrome coronavirus(MERS-CoV)is achieved by the detection of the viral antigens...Precise diagnosis and immunity to viruses,such as severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)and Middle East respiratory syndrome coronavirus(MERS-CoV)is achieved by the detection of the viral antigens and/or corresponding antibodies,respectively.However,a widely used antigen detection methods,such as polymerase chain reaction(PCR),are complex,expensive,and time-consuming Furthermore,the antibody test that detects an asymptomatic infection and immunity is usually performed separately and exhibits relatively low accuracy.To achieve a simplified,rapid,and accurate diagnosis,we have demonstrated an indium gallium zinc oxide(IGZO)-based biosensor field-effect transistor(bio-FET)that can simultaneously detect spike proteins and antibodies with a limit of detection(LOD)of 1 pg mL–1 and 200 ng mL–1,respectively using a single assay in less than 20 min by integrat-ing microfluidic channels and artificial neural networks(ANNs).The near-sensor ANN-aided classification provides high diagnosis accuracy(>93%)with significantly reduced processing time(0.62%)and energy consumption(5.64%)compared to the software-based ANN.We believe that the development of rapid and accurate diagnosis system for the viral antigens and antibodies detec-tion will play a crucial role in preventing global viral outbreaks.展开更多
The second affiliation of the authors in the original version of this article was unfortunately wrongly written on the first page, instead of Multi-Functional Nano/Bio Electronics Lab., Kyung Hee University, Gyeonggi ...The second affiliation of the authors in the original version of this article was unfortunately wrongly written on the first page, instead of Multi-Functional Nano/Bio Electronics Lab., Kyung Hee University, Gyeonggi 17104, Republic of Korea.展开更多
文摘Various approaches have been proposed for point-of-care diagnostics, and in particular, optical detection is preferred because it is relatively simple and fast. At the same time, field-effect transistor （FET）-based biosensors have attracted great attention because they can provide highly sensitive and label-free detection. In this work we present highly sensitive, epidermal skin-type point-of-care devices with system-level integration of flexible MOS2 FET biosensors, read-out circuits, and light-emitting diode （LEDs） that enable real-time detection of prostate cancer antigens （PSA）. Regardless of the physical forms or mechanical stress conditions, our proposed high-performance MoS2 biosensors can detect a PSA concentration of 1 pg.mL-1 without specific surface treatment for anti-PSA immobilization on the MoS2 surface on which we characterize and confirm physisorption of anti-PSA using Kelvin probe force microscopy （KPFM） and tapping-mode atomic force microscopy （tm-AFM）. Furthermore, current modulation induced by the binding process was stably maintained for longer than 2-3 min. The results indicate that flexible MoS2-based FET biosensors have great potential for point-of-care diagnostics for prostate cancer as well as other biomarkers.
基金National Science Foundation(NSF),Grant/Award Number:1942868IVY Foundation COVID-19 Translational Research Fund,Grant/Award Number:IVY14National Research Foundation of Korea(NRF),Grant/Award Number:NRF-2021R1A4A1033155。
文摘Precise diagnosis and immunity to viruses,such as severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)and Middle East respiratory syndrome coronavirus(MERS-CoV)is achieved by the detection of the viral antigens and/or corresponding antibodies,respectively.However,a widely used antigen detection methods,such as polymerase chain reaction(PCR),are complex,expensive,and time-consuming Furthermore,the antibody test that detects an asymptomatic infection and immunity is usually performed separately and exhibits relatively low accuracy.To achieve a simplified,rapid,and accurate diagnosis,we have demonstrated an indium gallium zinc oxide(IGZO)-based biosensor field-effect transistor(bio-FET)that can simultaneously detect spike proteins and antibodies with a limit of detection(LOD)of 1 pg mL–1 and 200 ng mL–1,respectively using a single assay in less than 20 min by integrat-ing microfluidic channels and artificial neural networks(ANNs).The near-sensor ANN-aided classification provides high diagnosis accuracy(>93%)with significantly reduced processing time(0.62%)and energy consumption(5.64%)compared to the software-based ANN.We believe that the development of rapid and accurate diagnosis system for the viral antigens and antibodies detec-tion will play a crucial role in preventing global viral outbreaks.
文摘The second affiliation of the authors in the original version of this article was unfortunately wrongly written on the first page, instead of Multi-Functional Nano/Bio Electronics Lab., Kyung Hee University, Gyeonggi 17104, Republic of Korea.
