β-Poly(L-malic acid)(PMLA)is a water-soluble biopolymer used in food,medicine and other industries.To date,the biosynthesis pathway of PMLA has not been fully elucidated.In this study,we sequenced the transcriptom e ...β-Poly(L-malic acid)(PMLA)is a water-soluble biopolymer used in food,medicine and other industries.To date,the biosynthesis pathway of PMLA has not been fully elucidated.In this study,we sequenced the transcriptom e of strain Aureobasidium melanogenum under 20 g/L CaCO_(3) addition.The resulting sequencing reads were assembled and annotated for the differentially expressed genes(DEGs)analysis and novel transcripts identification.The result indicated that with the CaCO_(3) addition,the tricarboxylic cycle(TCA)cycle and glyoxylate pathway were up-regulated,and it also found that a non-ribosomal peptide synthetase(NRPS)like protein was highly expressed.The DEGs analysis showed a high expression level of malate dehydrogenase(MDHC)and phosphoenolpyruvate carboxykinase(PCKA)in the CaCO_(3) group,which indicated a cytosolic malate activity.We speculated that the malate should be transported to or synthesized in the cytoplasm,which was then polymerized to PMLA by the NRPS-like protein,accompanied by the up-regulated TCA cycle providing ATP for the polymerization.Depending on the analysis,we assumed that an NRPS-like protein,the TCA cycle,and the cytosolic malate together are contributing to the PMLA biosynthesis.展开更多
探讨中心静脉血二氧化碳分压-动脉血二氧化碳分压差/动脉血氧含量-静脉血氧含量差[P(cv-a)CO_(2)/C(a-v)O_(2)ratio]在感染性休克早期目标导向性复苏中的应用。方法收集自2018年6月至2019年6月在新疆维吾尔自治区人民医院ICU接受治疗的...探讨中心静脉血二氧化碳分压-动脉血二氧化碳分压差/动脉血氧含量-静脉血氧含量差[P(cv-a)CO_(2)/C(a-v)O_(2)ratio]在感染性休克早期目标导向性复苏中的应用。方法收集自2018年6月至2019年6月在新疆维吾尔自治区人民医院ICU接受治疗的感染性休克患者44例。根据P(cv-a)CO_(2)/C(a-v)O_(2)ratio值将患者分为A、B 2组,其中A组为P(cv-a)CO_(2)/C(a-v)O_(2)ratio<1.6,B组为P(cv-a)CO_(2)/C(a-v)O_(2)ratio≥1.6。比较2组患者的基线资料[性别、年龄、感染部位、急性生理学与慢性健康状况(APACHEⅡ)评分与序贯器官衰竭评估(SOFA)评分]、基本参数[中心静脉压(CVP)、平均动脉压(MAP)、小时尿量、血管活性药物使用量、24 h出入量、血红蛋白、乳酸水平]、氧代谢参数[0、6、12、24 h的动脉血氧分压(PaO_(2)),动脉血氧饱和度(SaO_(2)),中心静脉血氧分压(PcvO_(2)),中心静脉血氧饱和度(ScvO_(2)),P(cv-a)CO_(2)(gap),P(cv-a)CO_(2)/C(a-v)O_(2)ratio,氧合指数,氧摄取率,及6、12 h的乳酸清除率]及转归[血管活性药物使用时间,机械通气时间,24、48、72、96 h的APACHEⅡ评分和SOFA评分,住ICU时间,住院时间,28 d病死率]等数据。结果2组患者基线资料比较差异无统计学意义(P>0.05)。0 h 2组间P(cv-a)CO_(2)/C(a-v)O_(2)ratio、氧分压、氧合指数、氧摄取率、乳酸、P(cv-a)CO_(2)(gap)比较,差异均有统计学意义(P<0.05);6 h 2组间乳酸清除率比较,差异有统计学意义(P<0.05)。2组间1、3、5、7 d APACHEⅡ评分、SOFA评分、血管活性药物使用时间(去甲肾上腺素)、机械通气时间、住ICU时间、住院时间及28 d病死率比较,差异均无统计学意义(P>0.05)。结论P(cv-a)CO_(2)/C(a-v)O_(2)ratio在感染性休克早期目标导向性复苏中可作为补充指标,评价组织乏氧代谢,指导治疗。展开更多
基金the financial support of the Tianjin Municipal Science and Technology Commission(17PTGCCX00190,17PTSYJC00080,17YFCZZC00310,and 16YFXTSF00460)the Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control(ZXKF20180301).
文摘β-Poly(L-malic acid)(PMLA)is a water-soluble biopolymer used in food,medicine and other industries.To date,the biosynthesis pathway of PMLA has not been fully elucidated.In this study,we sequenced the transcriptom e of strain Aureobasidium melanogenum under 20 g/L CaCO_(3) addition.The resulting sequencing reads were assembled and annotated for the differentially expressed genes(DEGs)analysis and novel transcripts identification.The result indicated that with the CaCO_(3) addition,the tricarboxylic cycle(TCA)cycle and glyoxylate pathway were up-regulated,and it also found that a non-ribosomal peptide synthetase(NRPS)like protein was highly expressed.The DEGs analysis showed a high expression level of malate dehydrogenase(MDHC)and phosphoenolpyruvate carboxykinase(PCKA)in the CaCO_(3) group,which indicated a cytosolic malate activity.We speculated that the malate should be transported to or synthesized in the cytoplasm,which was then polymerized to PMLA by the NRPS-like protein,accompanied by the up-regulated TCA cycle providing ATP for the polymerization.Depending on the analysis,we assumed that an NRPS-like protein,the TCA cycle,and the cytosolic malate together are contributing to the PMLA biosynthesis.
文摘探讨中心静脉血二氧化碳分压-动脉血二氧化碳分压差/动脉血氧含量-静脉血氧含量差[P(cv-a)CO_(2)/C(a-v)O_(2)ratio]在感染性休克早期目标导向性复苏中的应用。方法收集自2018年6月至2019年6月在新疆维吾尔自治区人民医院ICU接受治疗的感染性休克患者44例。根据P(cv-a)CO_(2)/C(a-v)O_(2)ratio值将患者分为A、B 2组,其中A组为P(cv-a)CO_(2)/C(a-v)O_(2)ratio<1.6,B组为P(cv-a)CO_(2)/C(a-v)O_(2)ratio≥1.6。比较2组患者的基线资料[性别、年龄、感染部位、急性生理学与慢性健康状况(APACHEⅡ)评分与序贯器官衰竭评估(SOFA)评分]、基本参数[中心静脉压(CVP)、平均动脉压(MAP)、小时尿量、血管活性药物使用量、24 h出入量、血红蛋白、乳酸水平]、氧代谢参数[0、6、12、24 h的动脉血氧分压(PaO_(2)),动脉血氧饱和度(SaO_(2)),中心静脉血氧分压(PcvO_(2)),中心静脉血氧饱和度(ScvO_(2)),P(cv-a)CO_(2)(gap),P(cv-a)CO_(2)/C(a-v)O_(2)ratio,氧合指数,氧摄取率,及6、12 h的乳酸清除率]及转归[血管活性药物使用时间,机械通气时间,24、48、72、96 h的APACHEⅡ评分和SOFA评分,住ICU时间,住院时间,28 d病死率]等数据。结果2组患者基线资料比较差异无统计学意义(P>0.05)。0 h 2组间P(cv-a)CO_(2)/C(a-v)O_(2)ratio、氧分压、氧合指数、氧摄取率、乳酸、P(cv-a)CO_(2)(gap)比较,差异均有统计学意义(P<0.05);6 h 2组间乳酸清除率比较,差异有统计学意义(P<0.05)。2组间1、3、5、7 d APACHEⅡ评分、SOFA评分、血管活性药物使用时间(去甲肾上腺素)、机械通气时间、住ICU时间、住院时间及28 d病死率比较,差异均无统计学意义(P>0.05)。结论P(cv-a)CO_(2)/C(a-v)O_(2)ratio在感染性休克早期目标导向性复苏中可作为补充指标,评价组织乏氧代谢,指导治疗。
