Epilepsy affects all age groups and is one of the most common and disabling neurological disorders worldwide.Drug-resistant epilepsy(DRE),status epilepticus(SE),and sudden unexpected death in epilepsy(SUDEP),which are...Epilepsy affects all age groups and is one of the most common and disabling neurological disorders worldwide.Drug-resistant epilepsy(DRE),status epilepticus(SE),and sudden unexpected death in epilepsy(SUDEP),which are associated with considerable healthcare costs and mortality,have always been difficult to address and become the focus of clinical research.The rapid identification of seizure onset and accurate localization of epileptic foci are crucial for the treatment and prognosis of people with DRE,SE,or near-SUDEP.However,most of the conventional neuroimaging techniques for assessing cerebral blood flow of people with epilepsy are restricted by time consumption,limited resolution,and ionizing radiation.Arterial spin labeling(ASL)is a newly powerful non-contrast magnetic resonance imaging technique that enables the quantitative evaluation of brain perfusion,characterized by its unique advantages of reproducibility and easy accessibility.Recent studies have demonstrated the potential advantages of ASL for the diagnosis and evaluation of epilepsy.Therefore,in this review,we discussed the complementary value of ASL in evaluating and characterizing the basic substrates underlying refractory epilepsy and epileptic emergencies.展开更多
目的揭示萎缩性阴道炎患者阴道群落特征,并探讨阴道菌群结构与萎缩性阴道炎发病之间的潜在关联。方法共纳入30名萎缩性阴道炎患者参与本研究(萎缩性阴道炎组),另有30名进行体检的绝经后健康妇女作为对照组。以16S r RNA基因为研究靶点,...目的揭示萎缩性阴道炎患者阴道群落特征,并探讨阴道菌群结构与萎缩性阴道炎发病之间的潜在关联。方法共纳入30名萎缩性阴道炎患者参与本研究(萎缩性阴道炎组),另有30名进行体检的绝经后健康妇女作为对照组。以16S r RNA基因为研究靶点,采用Illumina第二代高通量测序技术结合生物信息学分析,得阴道菌群结构数据。采用正则变量的逐步判别分析,对两组间群落组成的关联和差异进行比较。采用Spearman等级回归分析,探讨单个物种数量与疾病严重程度间的关系。结果在所有60例中,总共测得288个属的细菌。萎缩性阴道炎组和对照组间阴道菌群总体结构差异有统计学意义(P<0.05)。其中12个属的主要细菌相对丰度差异显著。在绝经后正常妇女的阴道内,乳酸杆菌占据整个阴道微生物群落主导地位。萎缩性阴道炎组阴道加德纳菌相对丰度(41.7%)显著高于对照组(16.7%,P<0.000 1),并取代乳酸杆菌成为阴道群落中生物优势最大的种群。萎缩性阴道炎组的乳酸杆菌失去了其生物优势地位,相对丰度(11.2%)显著低于对照组(53.2%,P<0.000 1)。相关性分析显示,萎缩性阴道炎组患者生殖道症状的严重程度与乳酸杆菌的相对丰度呈显著负相关(r=-0.301,P<0.001),而与加德纳菌(r=0.278,P<0.001)及奇异菌属(r=0.166,P<0.05)的数量呈显著正相关。结论阴道微生物群落失衡与萎缩性阴道炎发病存在关联。对于绝经后女性而言,处于生物优势地位的乳酸杆菌在维持阴道健康方面起到更为重要的作用。而阴道加德纳菌属和奇异菌属与绝经后妇女生殖道萎缩症状的严重程度有关,且这2种微生物的过度生长可能增加了罹患萎缩性阴道炎的风险。展开更多
文摘Epilepsy affects all age groups and is one of the most common and disabling neurological disorders worldwide.Drug-resistant epilepsy(DRE),status epilepticus(SE),and sudden unexpected death in epilepsy(SUDEP),which are associated with considerable healthcare costs and mortality,have always been difficult to address and become the focus of clinical research.The rapid identification of seizure onset and accurate localization of epileptic foci are crucial for the treatment and prognosis of people with DRE,SE,or near-SUDEP.However,most of the conventional neuroimaging techniques for assessing cerebral blood flow of people with epilepsy are restricted by time consumption,limited resolution,and ionizing radiation.Arterial spin labeling(ASL)is a newly powerful non-contrast magnetic resonance imaging technique that enables the quantitative evaluation of brain perfusion,characterized by its unique advantages of reproducibility and easy accessibility.Recent studies have demonstrated the potential advantages of ASL for the diagnosis and evaluation of epilepsy.Therefore,in this review,we discussed the complementary value of ASL in evaluating and characterizing the basic substrates underlying refractory epilepsy and epileptic emergencies.
文摘目的揭示萎缩性阴道炎患者阴道群落特征,并探讨阴道菌群结构与萎缩性阴道炎发病之间的潜在关联。方法共纳入30名萎缩性阴道炎患者参与本研究(萎缩性阴道炎组),另有30名进行体检的绝经后健康妇女作为对照组。以16S r RNA基因为研究靶点,采用Illumina第二代高通量测序技术结合生物信息学分析,得阴道菌群结构数据。采用正则变量的逐步判别分析,对两组间群落组成的关联和差异进行比较。采用Spearman等级回归分析,探讨单个物种数量与疾病严重程度间的关系。结果在所有60例中,总共测得288个属的细菌。萎缩性阴道炎组和对照组间阴道菌群总体结构差异有统计学意义(P<0.05)。其中12个属的主要细菌相对丰度差异显著。在绝经后正常妇女的阴道内,乳酸杆菌占据整个阴道微生物群落主导地位。萎缩性阴道炎组阴道加德纳菌相对丰度(41.7%)显著高于对照组(16.7%,P<0.000 1),并取代乳酸杆菌成为阴道群落中生物优势最大的种群。萎缩性阴道炎组的乳酸杆菌失去了其生物优势地位,相对丰度(11.2%)显著低于对照组(53.2%,P<0.000 1)。相关性分析显示,萎缩性阴道炎组患者生殖道症状的严重程度与乳酸杆菌的相对丰度呈显著负相关(r=-0.301,P<0.001),而与加德纳菌(r=0.278,P<0.001)及奇异菌属(r=0.166,P<0.05)的数量呈显著正相关。结论阴道微生物群落失衡与萎缩性阴道炎发病存在关联。对于绝经后女性而言,处于生物优势地位的乳酸杆菌在维持阴道健康方面起到更为重要的作用。而阴道加德纳菌属和奇异菌属与绝经后妇女生殖道萎缩症状的严重程度有关,且这2种微生物的过度生长可能增加了罹患萎缩性阴道炎的风险。
