Background:Radiotherapy is commonly used to treat cancers.To date,there has been no study focusing on the effects of radiotherapy on hydatid disease in large animals.In this study,we aim to investigate the efficiency ...Background:Radiotherapy is commonly used to treat cancers.To date,there has been no study focusing on the effects of radiotherapy on hydatid disease in large animals.In this study,we aim to investigate the efficiency and safety of radiotherapy for treating hydatid disease caused by Echinococcus granulosus in naturally infected sheep.Methods:Ultrasound was used to screen naturally infected sheep in an echinococcosis endemic area in Xinjiang,China.A computer tomography(CT)scan confirmed the presence of hydatid cysts.Twenty sheep naturally infected with E.granulosus in the liver and/or lungs were randomly assigned into four groups receiving no irradiation,or X-ray irradiation of low(30 Gy),medium(45 Gy),and high dose(60 Gy),respectively.After three months of radiotherapy,a CT scan was performed to measure the changes in the cysts.The hepatic parasite cysts and host tissues were collected for histology and gene expression analysis.Results:In the animals subject to irradiation,no significant differences were observed in their appetite,daily activities,and weight before and after radiotherapy.Severe calcification was noticed in the cysts subject to a high dose of radiation compared with the groups subject to low and medium doses.Hematoxylin and eosin staining showed that irradiation contributed to the damage of the cyst structure and nucleus in the germinal layers.Quantitative PCR demonstrated that expression of TPX and HSP70 significantly decreased in a dose-dependent manner(P<0.05).The expression of the EPC1 decreased in the medium-and high-dose groups compared with the low-dose group(P<0.05).Meanwhile,the expression of radiation-related apoptosis genes caspase-3 and Gadd45 decreased with an increase in the irradiation dose.Conclusions:Radiotherapy is an option with satisfactory efficiency and safety for treating cystic echinococcosis in sheep with partial response or stable disease at month 3.In future,inhibition of cystic activity using radiotherapy may serve as a new regimen for treating hydatid disease.展开更多
文摘目的基于第二版前列腺影像报告数据系统(PI-RAVS V2)对比双参数磁共振成像(BP-MRI)和多参数磁共振成像(MP-MRI)对前列腺癌(PCa)的诊断价值。方法选取2015年3月至2017年12月南京医科大学附属淮安第一医院收治的66例未经治疗临床怀疑PCa的患者进行MRI检查,包括T2加权成像(T2WI)、扩散加权成像(DWI)及动态对比增强磁共振成像(DCE-MRI),均经前列腺活检确定病理结果。两名放射科医师基于PI-RADS V2对BP-MRI及MP-MRI方案的诊断性能进行评估。结果病理证实27例PCa,其中23例位于外周带,4例位于移行带。BP-MRI检测PCa的灵敏度、特异度、准确度、阳性预测值、阴性预测值分别为90.6%(25/27)、97.7%(38/39)、92.4%(61/66)、95.8%(23/24)、94.7%(36/38),MP-MRI为87.2%(23/27)、95.3%(36/39)、93.9%(62/66)、89.3%(25/28)、90.5%(34/43)。结论与常规MP-MRI方案相比,3.0 T BP-MRI方案具有相似的诊断精度,检查时间较短且无需使用对比剂。
基金This work was supported by the Open Project of State Key Lab Incubation Base of Xinjiang Major Diseases Research(No.:SKLIB-XJMDR-2015-Y3).
文摘Background:Radiotherapy is commonly used to treat cancers.To date,there has been no study focusing on the effects of radiotherapy on hydatid disease in large animals.In this study,we aim to investigate the efficiency and safety of radiotherapy for treating hydatid disease caused by Echinococcus granulosus in naturally infected sheep.Methods:Ultrasound was used to screen naturally infected sheep in an echinococcosis endemic area in Xinjiang,China.A computer tomography(CT)scan confirmed the presence of hydatid cysts.Twenty sheep naturally infected with E.granulosus in the liver and/or lungs were randomly assigned into four groups receiving no irradiation,or X-ray irradiation of low(30 Gy),medium(45 Gy),and high dose(60 Gy),respectively.After three months of radiotherapy,a CT scan was performed to measure the changes in the cysts.The hepatic parasite cysts and host tissues were collected for histology and gene expression analysis.Results:In the animals subject to irradiation,no significant differences were observed in their appetite,daily activities,and weight before and after radiotherapy.Severe calcification was noticed in the cysts subject to a high dose of radiation compared with the groups subject to low and medium doses.Hematoxylin and eosin staining showed that irradiation contributed to the damage of the cyst structure and nucleus in the germinal layers.Quantitative PCR demonstrated that expression of TPX and HSP70 significantly decreased in a dose-dependent manner(P<0.05).The expression of the EPC1 decreased in the medium-and high-dose groups compared with the low-dose group(P<0.05).Meanwhile,the expression of radiation-related apoptosis genes caspase-3 and Gadd45 decreased with an increase in the irradiation dose.Conclusions:Radiotherapy is an option with satisfactory efficiency and safety for treating cystic echinococcosis in sheep with partial response or stable disease at month 3.In future,inhibition of cystic activity using radiotherapy may serve as a new regimen for treating hydatid disease.
