The growth, development and consumption of successive three generations of cotton bollworm, Helicoverpa armigera （Htibner）, fed on cotton bolls grown under elevated CO2 （double-ambient vs. ambient） in open-top cha...The growth, development and consumption of successive three generations of cotton bollworm, Helicoverpa armigera （Htibner）, fed on cotton bolls grown under elevated CO2 （double-ambient vs. ambient） in open-top chambers were examined. Significant decreases in protein, total amino acid, water and nitrogen content and increases in free fatty acid were observed in cotton bolls. Changes in quality of cotton bolls affected the growth, development and food utilization of H. armigera. Significantly longer larval development duration in three successive generations and lower pupal weight of the second and third generations were observed in cotton bollworm fed on cotton bolls grown under elevated CO2. Significantly lower fecundity was also found in successive three generations of H. armigera fed on cotton bolls grown under elevated CO2. The consumption per larva occurred significant increase in successive three generations and frass per larva were also significantly increased during the second and third generations under elevated CO2. Significantly lower relative growth rate, efficiency of conversion of ingested food and significant higher relative consumption rate in successive three generations were observed in cotton bollworm fed on cotton bolls grown under elevated CO2. Significantly lower potential female fecundity, larval numbers and population consumption were found in the second and third generations of cotton bollworm fed on cotton bolls grown under elevated CO2. The integrative effect of higher larval mortality rate and lower adult fecundity resulted in significant decreases in potential population consumption in the latter two generations. The results show that elevated CO2 adversely affects cotton bolls quality, which indicates the potential population dynamics and potential population consumption of cotton bollworm will alleviate the harm to the plants in the future rising CO2 atmosphere.展开更多
Cockroaches are worldwide indoor pests carrying microorganisms of medical importance. German cockroaches （Blattella germanica） were sampled in five habitats （hospital, restaurant, office home, and market） in Beiji...Cockroaches are worldwide indoor pests carrying microorganisms of medical importance. German cockroaches （Blattella germanica） were sampled in five habitats （hospital, restaurant, office home, and market） in Beijing, and the bacteria were isolated from their external surface and alimentary tract and identified using a Biolog identification system. Cockroach densities significantly differed among habitats （market 〉 home 〉 office 〉 restaurant 〉 hospital）. However, no significant differences in bacterial abundance carried by individual German cockroaches （of either sex） were found among habitats. The bacterial abundance in the gut was significantly higher than that on the surface. There were no significant differences in bacterial species richness observed among habitats, sex, carrying position or their interaction. Cluster analysis showed that cockroach densities and bacterial abundance found in the market differed significantly from the other four habitats. The bacterial diversity was not significantly reduced in sensitive facilities such as hospital and restaurant, even though pesticide and bactericide were more frequently applied there. The implications of these findings were discussed in this article.展开更多
目的阐释三七(PN)对耐甲氧西林金黄色葡萄球菌(MRSA)菌株ATCC43300(医院获得性)和USA300(社区获得性)的药效差异及分子机制。方法采用RNA-Seq技术分析PN干预对两株MRSA转录组的影响,以|log_(2)FC|≥1.0且FDR<0.05为筛选标准,鉴定差...目的阐释三七(PN)对耐甲氧西林金黄色葡萄球菌(MRSA)菌株ATCC43300(医院获得性)和USA300(社区获得性)的药效差异及分子机制。方法采用RNA-Seq技术分析PN干预对两株MRSA转录组的影响,以|log_(2)FC|≥1.0且FDR<0.05为筛选标准,鉴定差异表达基因(DEGs)。进一步结合gene ontology(GO)和kyoto encyclopedia of genes and genomes(KEGG)功能富集分析、加权基因共表达网络分析(WGCNA)及蛋白质互作(PPI)网络分析,识别相关的关键通路与核心调控基因。结果在ATCC43300中,共鉴定出478个差异表达基因,其中上调基因201个,下调基因277个;在USA300中,鉴定出274个差异表达基因,其中上调基因130个,下调基因144个。ATCC43300中,支链氨基酸代谢和膜脂代谢通路显著上调;而在USA300中,嘌呤代谢和群体感应通路显著下调。WGCNA分析识别出与PN干预相关的核心模块,ATCC43300的核心模块富集在膜脂代谢相关基因,而USA300则富集在氨基酸代谢通路。PPI分析揭示,ATCC43300中与膜脂代谢和氧化还原相关的多个通路受到影响,而USA300中,嘌呤代谢与群体感应系统相关的多个基因通路受到抑制,表明PN可能通过干扰这些关键代谢和信号通路,削弱细菌的致病能力。结论PN可诱导MRSA菌株的多个代谢通路变化,表现出对不同菌株的代谢重构效应。ATCC43300中,支链氨基酸代谢与膜脂合成通路显著上调,可能通过增强代谢负担和氧化应激反应干扰其膜结构稳定性与代谢稳态,从而削弱其生存适应性并影响耐药相关功能通路。USA300中,嘌呤代谢与群体感应系统显著下调,推测PN可通过抑制毒力因子表达削弱其致病能力。本研究揭示了PN对MRSA菌株的差异化转录调控机制,为其在不同MRSA菌株感染中的精准应用提供理论依据。展开更多
Patients with periodontal disease often require combined periodontal-orthodontic interventions to restore periodontal health,function,and aesthetics,ensuring both patient satisfaction and long-term stability.Managing ...Patients with periodontal disease often require combined periodontal-orthodontic interventions to restore periodontal health,function,and aesthetics,ensuring both patient satisfaction and long-term stability.Managing these patients involving orthodontic tooth movement can be particularly challenging due to compromised periodontal soft and hard tissues,especially in severe cases.Therefore,close collaboration between orthodontists and periodontists for comprehensive diagnosis and sequential treatment,along with diligent patient compliance throughout the entire process,is crucial for achieving favorable treatment outcomes.Moreover,long-term orthodontic retention and periodontal follow-up are essential to sustain treatment success.This expert consensus,informed by the latest clinical research and practical experience,addresses clinical considerations for orthodontic treatment of periodontal patients,delineating indications,objectives,procedures,and principles with the aim of providing clear and practical guidance for clinical practitioners.展开更多
基金Project supported by the National Basic Research Program(973)of China(No.2006CB102002)the Pilot Project of Knowledge Innovation Program of Chinese Academy of Sciences(No.KSCX2-YW-N-006)National Natural Science Foundation of China(No.30571253,30621003)
文摘The growth, development and consumption of successive three generations of cotton bollworm, Helicoverpa armigera （Htibner）, fed on cotton bolls grown under elevated CO2 （double-ambient vs. ambient） in open-top chambers were examined. Significant decreases in protein, total amino acid, water and nitrogen content and increases in free fatty acid were observed in cotton bolls. Changes in quality of cotton bolls affected the growth, development and food utilization of H. armigera. Significantly longer larval development duration in three successive generations and lower pupal weight of the second and third generations were observed in cotton bollworm fed on cotton bolls grown under elevated CO2. Significantly lower fecundity was also found in successive three generations of H. armigera fed on cotton bolls grown under elevated CO2. The consumption per larva occurred significant increase in successive three generations and frass per larva were also significantly increased during the second and third generations under elevated CO2. Significantly lower relative growth rate, efficiency of conversion of ingested food and significant higher relative consumption rate in successive three generations were observed in cotton bollworm fed on cotton bolls grown under elevated CO2. Significantly lower potential female fecundity, larval numbers and population consumption were found in the second and third generations of cotton bollworm fed on cotton bolls grown under elevated CO2. The integrative effect of higher larval mortality rate and lower adult fecundity resulted in significant decreases in potential population consumption in the latter two generations. The results show that elevated CO2 adversely affects cotton bolls quality, which indicates the potential population dynamics and potential population consumption of cotton bollworm will alleviate the harm to the plants in the future rising CO2 atmosphere.
基金supported by the Knowledge Innovation Program of CAS (No. KZCX2-YW-422)the Open Fund of State Key Laboratory of Urban and Regional Ecology.
文摘Cockroaches are worldwide indoor pests carrying microorganisms of medical importance. German cockroaches （Blattella germanica） were sampled in five habitats （hospital, restaurant, office home, and market） in Beijing, and the bacteria were isolated from their external surface and alimentary tract and identified using a Biolog identification system. Cockroach densities significantly differed among habitats （market 〉 home 〉 office 〉 restaurant 〉 hospital）. However, no significant differences in bacterial abundance carried by individual German cockroaches （of either sex） were found among habitats. The bacterial abundance in the gut was significantly higher than that on the surface. There were no significant differences in bacterial species richness observed among habitats, sex, carrying position or their interaction. Cluster analysis showed that cockroach densities and bacterial abundance found in the market differed significantly from the other four habitats. The bacterial diversity was not significantly reduced in sensitive facilities such as hospital and restaurant, even though pesticide and bactericide were more frequently applied there. The implications of these findings were discussed in this article.
文摘目的阐释三七(PN)对耐甲氧西林金黄色葡萄球菌(MRSA)菌株ATCC43300(医院获得性)和USA300(社区获得性)的药效差异及分子机制。方法采用RNA-Seq技术分析PN干预对两株MRSA转录组的影响,以|log_(2)FC|≥1.0且FDR<0.05为筛选标准,鉴定差异表达基因(DEGs)。进一步结合gene ontology(GO)和kyoto encyclopedia of genes and genomes(KEGG)功能富集分析、加权基因共表达网络分析(WGCNA)及蛋白质互作(PPI)网络分析,识别相关的关键通路与核心调控基因。结果在ATCC43300中,共鉴定出478个差异表达基因,其中上调基因201个,下调基因277个;在USA300中,鉴定出274个差异表达基因,其中上调基因130个,下调基因144个。ATCC43300中,支链氨基酸代谢和膜脂代谢通路显著上调;而在USA300中,嘌呤代谢和群体感应通路显著下调。WGCNA分析识别出与PN干预相关的核心模块,ATCC43300的核心模块富集在膜脂代谢相关基因,而USA300则富集在氨基酸代谢通路。PPI分析揭示,ATCC43300中与膜脂代谢和氧化还原相关的多个通路受到影响,而USA300中,嘌呤代谢与群体感应系统相关的多个基因通路受到抑制,表明PN可能通过干扰这些关键代谢和信号通路,削弱细菌的致病能力。结论PN可诱导MRSA菌株的多个代谢通路变化,表现出对不同菌株的代谢重构效应。ATCC43300中,支链氨基酸代谢与膜脂合成通路显著上调,可能通过增强代谢负担和氧化应激反应干扰其膜结构稳定性与代谢稳态,从而削弱其生存适应性并影响耐药相关功能通路。USA300中,嘌呤代谢与群体感应系统显著下调,推测PN可通过抑制毒力因子表达削弱其致病能力。本研究揭示了PN对MRSA菌株的差异化转录调控机制,为其在不同MRSA菌株感染中的精准应用提供理论依据。
基金supported by the National Key R&D Program of China(2022YFC2504200)Chongqing Young and Middle-aged Medical Excellence Team ProjectJiangsu Province Key Research and Development Program(BE2022670)。
文摘Patients with periodontal disease often require combined periodontal-orthodontic interventions to restore periodontal health,function,and aesthetics,ensuring both patient satisfaction and long-term stability.Managing these patients involving orthodontic tooth movement can be particularly challenging due to compromised periodontal soft and hard tissues,especially in severe cases.Therefore,close collaboration between orthodontists and periodontists for comprehensive diagnosis and sequential treatment,along with diligent patient compliance throughout the entire process,is crucial for achieving favorable treatment outcomes.Moreover,long-term orthodontic retention and periodontal follow-up are essential to sustain treatment success.This expert consensus,informed by the latest clinical research and practical experience,addresses clinical considerations for orthodontic treatment of periodontal patients,delineating indications,objectives,procedures,and principles with the aim of providing clear and practical guidance for clinical practitioners.
