Objective:Recent cholera outbreaks in Anhui Province have been linked to Vibrio cholerae O139,but information on these strains is limited.We established the first genomic dataset of local O139 strains to analyze the g...Objective:Recent cholera outbreaks in Anhui Province have been linked to Vibrio cholerae O139,but information on these strains is limited.We established the first genomic dataset of local O139 strains to analyze the genomic characteristics and evolution of antibiotic resistance.Methods:Thirty-four Vibrio cholerae O139 isolates from Anhui(2013–2024)were sequenced using nextgeneration sequencing.Genes for virulence,antimicrobial resistance,pathogenicity islands,and mobile genetic elements were predicted using ABRicate and other online tools.To construct a phylogenetic tree,124 publicly available O139 genomes were included in the single-nucleotide polymorphism analysis alongside the study isolates.Results:Strains formed two clusters that were genetically closer to China isolates than with those from Bangladesh and India.All strains harbored ctxA and ctxB,with partial deletions in virulence genes and pathogenicity islands;over 50%lacked vgrG-2 in the T6SS.Strains from 2022–2024 exhibited higher azithromycin but lower trimethoprimsulfamethoxazole resistance than those collected during 2013–2017.Conclusion:Vibrio cholerae O139 in Anhui are endemic to China,with limited virulence but strong colonization abilities.The increased azithromycin resistance rate may be driven by its clinical antimicrobial usage,suggesting its potential for continued antibiotic resistance evolution.展开更多
Introduction:On August 11,2025,a suspected Chikungunya virus(CHIKV)case traveled from Foshan to Fuyang Xiguan Airport,Anhui Province.After symptom reporting on August 12,local CDC launched epidemiological investigatio...Introduction:On August 11,2025,a suspected Chikungunya virus(CHIKV)case traveled from Foshan to Fuyang Xiguan Airport,Anhui Province.After symptom reporting on August 12,local CDC launched epidemiological investigations,laboratory testing,and control measures.Methods:We collected serial blood samples throughout the patient’s hospitalization and documented the complete clinical progression from admission to discharge.Real-time fluorescent quantitative polymerase chain reaction(qPCR)was employed to detect CHIKV nucleic acid,while Enzyme-Linked Immunosorbent Assay(ELISA)was used to test for CHIKV IgG and IgM antibodies.MinION nanopore sequencing was performed on blood samples to obtain the complete viral genome sequence.Phylogenetic analyses were subsequently constructed to determine the origin,genotype,and mutation profile of this imported case.Results:The qPCR analysis confirmed CHIKV presence in the patient’s serum samples.ELISA detected CHIKV IgG and IgM antibodies in blood samples collected on the fifth and ninth days after illness onset,respectively.Nanopore sequencing successfully generated the complete CHIKV genome sequence.Phylogenetic analysis demonstrated that the strain belonged to the East-Central-South-African lineage,consistent with the genotype identified from 190 cases in the local clustered Chikungunya fever(CHIKF)outbreak that occurred in Guangdong Province in July 2025.The strain showed 99.964% nucleotide identity(4 differential loci)with strain PX216392.1 detected in the current epidemic,and 99.9556% identity(5 differential loci)with PX236189.1.Conclusion:This is Anhui’s first imported CHIKV case,linked to the Guangdong outbreak.No local transmission or CHIKV-positive vector mosquitoes were detected.展开更多
基金Supported by the National Disease Control and Prevention Administration Public Health Talent Training Program(202303)the Open Fund of the State Key Laboratory of Tea Plant Biology and Utilization(SKLTOF20200131)+1 种基金the Scientific Research Projects of Health Commission of Anhui Province in 2022(AHWJ2022a022)the Scientific Research Projects of Health Commission of Anhui Province in 2023(AHWJ2023A20398).
文摘Objective:Recent cholera outbreaks in Anhui Province have been linked to Vibrio cholerae O139,but information on these strains is limited.We established the first genomic dataset of local O139 strains to analyze the genomic characteristics and evolution of antibiotic resistance.Methods:Thirty-four Vibrio cholerae O139 isolates from Anhui(2013–2024)were sequenced using nextgeneration sequencing.Genes for virulence,antimicrobial resistance,pathogenicity islands,and mobile genetic elements were predicted using ABRicate and other online tools.To construct a phylogenetic tree,124 publicly available O139 genomes were included in the single-nucleotide polymorphism analysis alongside the study isolates.Results:Strains formed two clusters that were genetically closer to China isolates than with those from Bangladesh and India.All strains harbored ctxA and ctxB,with partial deletions in virulence genes and pathogenicity islands;over 50%lacked vgrG-2 in the T6SS.Strains from 2022–2024 exhibited higher azithromycin but lower trimethoprimsulfamethoxazole resistance than those collected during 2013–2017.Conclusion:Vibrio cholerae O139 in Anhui are endemic to China,with limited virulence but strong colonization abilities.The increased azithromycin resistance rate may be driven by its clinical antimicrobial usage,suggesting its potential for continued antibiotic resistance evolution.
基金Supported by the Anhui Provincial Health Science and Technology Project(AHWJ2024Aa20203 and AHWJ2024Aa20208)the Key Health Research Program of Anhui Province in 2024(AHWJ2024Aa10092)the Peak Discipline Project from the School of Public Health,Anhui Medical University(Collaborative Education Innovation Project)(2024GWXTYRZ005).
文摘Introduction:On August 11,2025,a suspected Chikungunya virus(CHIKV)case traveled from Foshan to Fuyang Xiguan Airport,Anhui Province.After symptom reporting on August 12,local CDC launched epidemiological investigations,laboratory testing,and control measures.Methods:We collected serial blood samples throughout the patient’s hospitalization and documented the complete clinical progression from admission to discharge.Real-time fluorescent quantitative polymerase chain reaction(qPCR)was employed to detect CHIKV nucleic acid,while Enzyme-Linked Immunosorbent Assay(ELISA)was used to test for CHIKV IgG and IgM antibodies.MinION nanopore sequencing was performed on blood samples to obtain the complete viral genome sequence.Phylogenetic analyses were subsequently constructed to determine the origin,genotype,and mutation profile of this imported case.Results:The qPCR analysis confirmed CHIKV presence in the patient’s serum samples.ELISA detected CHIKV IgG and IgM antibodies in blood samples collected on the fifth and ninth days after illness onset,respectively.Nanopore sequencing successfully generated the complete CHIKV genome sequence.Phylogenetic analysis demonstrated that the strain belonged to the East-Central-South-African lineage,consistent with the genotype identified from 190 cases in the local clustered Chikungunya fever(CHIKF)outbreak that occurred in Guangdong Province in July 2025.The strain showed 99.964% nucleotide identity(4 differential loci)with strain PX216392.1 detected in the current epidemic,and 99.9556% identity(5 differential loci)with PX236189.1.Conclusion:This is Anhui’s first imported CHIKV case,linked to the Guangdong outbreak.No local transmission or CHIKV-positive vector mosquitoes were detected.
