[Object]This study aimed to examine the effects of 3,7-dimethyl-1-（5-oxo-hexyl）-xanthine（pentoxifylline,PF）on the motility,mitochondrial activity,acrosome integrity and fertilization rate of spermatozoa in both fr...[Object]This study aimed to examine the effects of 3,7-dimethyl-1-（5-oxo-hexyl）-xanthine（pentoxifylline,PF）on the motility,mitochondrial activity,acrosome integrity and fertilization rate of spermatozoa in both fresh and frozen-thawed bull semen.[Method]Fresh and frozen-thawed bull spermatozoa were exposed to 5 mmol/L PF with untreated samples as controls.[Result]Fresh spermatozoa showed reduced（P〈0.05）motility after 2 h incubation with PF whereas,surprisingly,frozen-thawed samples exhibited increased sperm motility（P〈0.05）after 2 h incubation with PF and they also showed enhanced longevity compared to controls.Mitochondrial activity in both fresh and frozen-thawed bull spermatozoa increased（P〈0.05）during 4 h incubation with PF whereas acrosome integrity remained unchanged in both types of semen.However,treatment with 5 mmol/L PF did not influence the in vitro fertilization efficiency of fresh spermatozoa but improved significantly（P〈0.05）that of frozen-thawed spermatozoa.[Conclusion]These results indicate that PF can improve sperm quality of frozenthawed bull semen,and may improve pregnancy rates in bovine artificial insemination programmes employing frozen semen.展开更多
Tumor-associated inflammation is an important component of the tumor microenvironment,and an important factor affecting tumor progression.In the tumor microenvironment,tumor-associated macrophages(TAMs)receive differe...Tumor-associated inflammation is an important component of the tumor microenvironment,and an important factor affecting tumor progression.In the tumor microenvironment,tumor-associated macrophages(TAMs)receive different stimuli and can be polarized into classically activated M1 macrophages and alternatively activated M2 macrophages.Many studies have indicated that the polarization of TAMs is closely related to tumor progression.展开更多
基金Supported by NMKJ Project of Production and Industrial Application of Sexed Semen in Domestic Animals(No.20111701)
文摘[Object]This study aimed to examine the effects of 3,7-dimethyl-1-（5-oxo-hexyl）-xanthine（pentoxifylline,PF）on the motility,mitochondrial activity,acrosome integrity and fertilization rate of spermatozoa in both fresh and frozen-thawed bull semen.[Method]Fresh and frozen-thawed bull spermatozoa were exposed to 5 mmol/L PF with untreated samples as controls.[Result]Fresh spermatozoa showed reduced（P〈0.05）motility after 2 h incubation with PF whereas,surprisingly,frozen-thawed samples exhibited increased sperm motility（P〈0.05）after 2 h incubation with PF and they also showed enhanced longevity compared to controls.Mitochondrial activity in both fresh and frozen-thawed bull spermatozoa increased（P〈0.05）during 4 h incubation with PF whereas acrosome integrity remained unchanged in both types of semen.However,treatment with 5 mmol/L PF did not influence the in vitro fertilization efficiency of fresh spermatozoa but improved significantly（P〈0.05）that of frozen-thawed spermatozoa.[Conclusion]These results indicate that PF can improve sperm quality of frozenthawed bull semen,and may improve pregnancy rates in bovine artificial insemination programmes employing frozen semen.
基金supported by the National Natural Science Foundation of China(No.81902435)Natural Science Foundation of Fujian Province of China(No.2021J011371)+4 种基金Sichuan Science and Technology Program of China(No.2021YFS0375,2023NSFSC1899)Science and technology project of Henan Province,China(No.222102310037)Henan Province Medical Science and Technology Project of China(No.LHGJ20220018)Medjaden Academy&Research Foundation for Young Scientists(China)(No.MJR20221014)Project of Sichuan Provincial People's Hospital(China)(No.2022QN03,2021LY14).
文摘Tumor-associated inflammation is an important component of the tumor microenvironment,and an important factor affecting tumor progression.In the tumor microenvironment,tumor-associated macrophages(TAMs)receive different stimuli and can be polarized into classically activated M1 macrophages and alternatively activated M2 macrophages.Many studies have indicated that the polarization of TAMs is closely related to tumor progression.
