Photothermal therapy(PTT)is a desirable way to attain on-demand hyperthermia owing to the heat gen-erated by photothermal materials absorbing near-infrared light.The mild heat(42±0.5℃)can not only accelerate bon...Photothermal therapy(PTT)is a desirable way to attain on-demand hyperthermia owing to the heat gen-erated by photothermal materials absorbing near-infrared light.The mild heat(42±0.5℃)can not only accelerate bone tissue regeneration but also promote the release of bioactive ions from biomaterials.Based on this one-stone-two-birds strategy,a 3D printed PEEK-graphene composite scaffold(PG)with hydroxyapatite(HA)coating(PGH)for photothermally remote control of bone regeneration was well de-signed in this study.The results showed that the HA coating on PGH could release Ca^(2+)and PO_(4)^(3-)ions easily under NIR irradiation,which was ascribed to the mild heat generated from graphene in the com-posite scaffold.The mild heat and the boosted Ca^(2+)/PO_(4)^(3-)release could synergistically enhance the bone regeneration ability both in vitro and in vivo.The underlying mechanism was further explored and con-firmed to be closely related to the upregulation of HSP 70,by which the MAPK/ERK signaling pathway was activated selectively.The favorable results demonstrate that the 3D-printed PEEK/graphene composite scaffold is promising in the applications of bone defect repair.展开更多
The authors regret that in Fig.2Aa of the article,the images for TPUPPy and TPU-aa in the first column were mistakenly duplicated due to a formatting error.The correct version of Fig.2 is provided below,along with the...The authors regret that in Fig.2Aa of the article,the images for TPUPPy and TPU-aa in the first column were mistakenly duplicated due to a formatting error.The correct version of Fig.2 is provided below,along with the original data for reference.The authors confirm that the rest of the article remains unaffected.展开更多
In this study,a novel artificial intervertebral disc implant with modified“Bucklicrystal”structure was designed and 3D printed using thermoplastic polyurethane.The new implant has a unique auxetic structure with bui...In this study,a novel artificial intervertebral disc implant with modified“Bucklicrystal”structure was designed and 3D printed using thermoplastic polyurethane.The new implant has a unique auxetic structure with building blocks joined“face-to-face”.The accompanied negative Poisson’s ratio enables its excellent energy absorption and stability under compression.The deformation and load distribution behavior of the implant under various loading conditions(bending,torsion,extension and flexion)has been thoroughly evaluated through finite element method.Results show that,compared to natural intervertebral disc and conventional 3D implant,our new implant exhibits more effective stress transfer and attenuation under practical loading conditions.The implant’s ability to contract laterally under compression can be potentially used to alleviate the symptoms of lumbar disc herniation.Finally,the biocompatibility of the implant was assessed in vitro and its ability to restore the physiological function of the disc segment was validated in vivo using an animal model.展开更多
Previous studies have confirmed that intervertebral disc degeneration(IDD)is closely associated with inflammation-induced reactive oxygen species(ROS)and resultant cell mitochondrial membrane potential(MMP)decline.Cle...Previous studies have confirmed that intervertebral disc degeneration(IDD)is closely associated with inflammation-induced reactive oxygen species(ROS)and resultant cell mitochondrial membrane potential(MMP)decline.Clearance of ROS in an inflammatory environment is essential for breaking the vicious cycle of MMP decline.Additionally,re-energizing the mitochondria damaged in the inflammatory milieu to restore their function,is equally important.Herein,we proposed an interesting concept of mitochondrion-engine equipped with coolant,which enables first to“cool-down”the inflammatory environment,next to restore the MMP,finally to allow cells to regain normal energy metabolism through materials design.As such,we developed a multifunctional composite composed of a reactive oxygen species(ROS)-responsive sodium alginate/gelatin hydrogel infused into a rigid 3D-printed thermoplastic polyurethane(TPU)scaffold.The TPU scaffold was coated with conductive polypyrrole(PPy)to electrophoretically deposit L-arginine,which could upregulate the Mammalian target of rapamycin(mTOR)pathway,thus increasing MMP and energy metabolism to stimulate extracellular matrix synthesis for IVD repair.While the ROS-responsive hydrogel acting as the“mito-engine coolant”could scavenge the excessive ROS to create a favorable environment for IVD cells recovery.Demonstrated by in vitro and in vivo evaluations,the mito-engine system markedly promoted the proliferation and collagen synthesis of nucleus pulposus cells while enhancing the mitochondrial respiration and MMP under oxidative stress.Radiological and histological assessments in vivo revealed the efficacy of this system in IVD repair.This unique bioinspired design integrated biomaterial science with mitochondrial biology,presents a promising paradigm for IDD treatment.展开更多
Delayed healing of diabetic wounds poses a major challenge to human health due to severe vascular dysfunction,sustained inflammation,and vulnerability to microbial infection.Herein,we constructed multidimensionally na...Delayed healing of diabetic wounds poses a major challenge to human health due to severe vascular dysfunction,sustained inflammation,and vulnerability to microbial infection.Herein,we constructed multidimensionally nano-topologized elec-trospun polycaprolactone(PCL)fibrous membranes with shish-kebab nanoarrays on each fiber through self-induced crys-tallization,on which the CuO2-MgO2 bimetallic peroxide nanodots(BPNs)were anchored by polydopamine(PDA)as the bridging layer.When activated by the acidic microenvironment(typically infected diabetic wound),BPNs on fibers reacted immediately to release Cu2+and Mg2+ions together with hydrogen peroxide(H2O2)molecules,which were then transferred into·OH radicals through Fenton-type reactions catalyzed by Cu2+for instant bacteria elimination.At the same time,the released Cu2+and Mg2+ions were retained to improve the angiogenesis and suppress the inflammation infiltration,thus remodeling the wound microenvironment.Meanwhile,the one-dimensional(1D)-constructed nano shish-kebabs and PDA coating on fibers provided additional topological activation for cell adhesion and directed migration along the aligned fiber orientation.Through the meticulous design,the resultant membranes markedly accelerated the infected wound healing in the diabetic rat model.This study pioneers a unique design to develop a nanocomposite fibrous membrane that combines multidimensional topologies with chemodynamic therapy(CDT),for efficiently combating infected diabetic wounds.展开更多
The authors regret that in Fig.2Aa of the article,the images for TPUPPy and TPU-aa in the first column were mistakenly duplicated due to a formatting error.The correct version of Fig.2 is provided below,along with the...The authors regret that in Fig.2Aa of the article,the images for TPUPPy and TPU-aa in the first column were mistakenly duplicated due to a formatting error.The correct version of Fig.2 is provided below,along with the original data for reference.The authors confirm that the rest of the article remains unaffected.展开更多
基金supported by grants from the Project funded by the China Postdoctoral Science Foundation(Nos.2023M732469 and 2023M732477)the Sichuan University Postdoctoral Interdisciplinary Innovation Fund(No.JCXK2205)+3 种基金the Projects of the Science and Technology Department of Sichuan Province(Nos.24NSFSC7945,24NSFSC6493,2022ZDZX0029,2023NSFSC0659,MZGC20230019)the National Natural Science Foundation of China(No.52302351)the Sichuan Provincial Medical Association Special Research Fund(Nos.2021SAT05 and 2019HR18)the National Program for Postdoctoral Researchers(No.GZB20230485)as well as the Fundamental Research Funds for the Central Universities(No.2023SCU12116).
文摘Photothermal therapy(PTT)is a desirable way to attain on-demand hyperthermia owing to the heat gen-erated by photothermal materials absorbing near-infrared light.The mild heat(42±0.5℃)can not only accelerate bone tissue regeneration but also promote the release of bioactive ions from biomaterials.Based on this one-stone-two-birds strategy,a 3D printed PEEK-graphene composite scaffold(PG)with hydroxyapatite(HA)coating(PGH)for photothermally remote control of bone regeneration was well de-signed in this study.The results showed that the HA coating on PGH could release Ca^(2+)and PO_(4)^(3-)ions easily under NIR irradiation,which was ascribed to the mild heat generated from graphene in the com-posite scaffold.The mild heat and the boosted Ca^(2+)/PO_(4)^(3-)release could synergistically enhance the bone regeneration ability both in vitro and in vivo.The underlying mechanism was further explored and con-firmed to be closely related to the upregulation of HSP 70,by which the MAPK/ERK signaling pathway was activated selectively.The favorable results demonstrate that the 3D-printed PEEK/graphene composite scaffold is promising in the applications of bone defect repair.
文摘The authors regret that in Fig.2Aa of the article,the images for TPUPPy and TPU-aa in the first column were mistakenly duplicated due to a formatting error.The correct version of Fig.2 is provided below,along with the original data for reference.The authors confirm that the rest of the article remains unaffected.
基金support from the National Natural Science Foundation of China(No.81772397,81871772,82072434)aSichuan Science and Technology Program(2021YFH0134,2020YFS0131).
文摘In this study,a novel artificial intervertebral disc implant with modified“Bucklicrystal”structure was designed and 3D printed using thermoplastic polyurethane.The new implant has a unique auxetic structure with building blocks joined“face-to-face”.The accompanied negative Poisson’s ratio enables its excellent energy absorption and stability under compression.The deformation and load distribution behavior of the implant under various loading conditions(bending,torsion,extension and flexion)has been thoroughly evaluated through finite element method.Results show that,compared to natural intervertebral disc and conventional 3D implant,our new implant exhibits more effective stress transfer and attenuation under practical loading conditions.The implant’s ability to contract laterally under compression can be potentially used to alleviate the symptoms of lumbar disc herniation.Finally,the biocompatibility of the implant was assessed in vitro and its ability to restore the physiological function of the disc segment was validated in vivo using an animal model.
基金the National Natural Science Foundation of China(82172495,82260431,82072434,82372453)Project funded by China Postdoctoral Science Foundation(2023M732469)+2 种基金Sichuan University Postdoctoral Interdisciplinary Innovation Fund(JCXK2205)Projects of the Science and Technology Department of Sichuan Province(2022ZDZX0029,MZGC20230019)the 1⋅3⋅5 project for disciplines of excellence Clinical Research Incubation Project,West China Hospital,Sichuan University(2021HXFH003).
文摘Previous studies have confirmed that intervertebral disc degeneration(IDD)is closely associated with inflammation-induced reactive oxygen species(ROS)and resultant cell mitochondrial membrane potential(MMP)decline.Clearance of ROS in an inflammatory environment is essential for breaking the vicious cycle of MMP decline.Additionally,re-energizing the mitochondria damaged in the inflammatory milieu to restore their function,is equally important.Herein,we proposed an interesting concept of mitochondrion-engine equipped with coolant,which enables first to“cool-down”the inflammatory environment,next to restore the MMP,finally to allow cells to regain normal energy metabolism through materials design.As such,we developed a multifunctional composite composed of a reactive oxygen species(ROS)-responsive sodium alginate/gelatin hydrogel infused into a rigid 3D-printed thermoplastic polyurethane(TPU)scaffold.The TPU scaffold was coated with conductive polypyrrole(PPy)to electrophoretically deposit L-arginine,which could upregulate the Mammalian target of rapamycin(mTOR)pathway,thus increasing MMP and energy metabolism to stimulate extracellular matrix synthesis for IVD repair.While the ROS-responsive hydrogel acting as the“mito-engine coolant”could scavenge the excessive ROS to create a favorable environment for IVD cells recovery.Demonstrated by in vitro and in vivo evaluations,the mito-engine system markedly promoted the proliferation and collagen synthesis of nucleus pulposus cells while enhancing the mitochondrial respiration and MMP under oxidative stress.Radiological and histological assessments in vivo revealed the efficacy of this system in IVD repair.This unique bioinspired design integrated biomaterial science with mitochondrial biology,presents a promising paradigm for IDD treatment.
基金supported by the National Key Research and Development Program of China(2021YFA1201300)the Scientific Fund for Distinguished Young Scholars in Sichuan Province(2022JDJQ0051).
文摘Delayed healing of diabetic wounds poses a major challenge to human health due to severe vascular dysfunction,sustained inflammation,and vulnerability to microbial infection.Herein,we constructed multidimensionally nano-topologized elec-trospun polycaprolactone(PCL)fibrous membranes with shish-kebab nanoarrays on each fiber through self-induced crys-tallization,on which the CuO2-MgO2 bimetallic peroxide nanodots(BPNs)were anchored by polydopamine(PDA)as the bridging layer.When activated by the acidic microenvironment(typically infected diabetic wound),BPNs on fibers reacted immediately to release Cu2+and Mg2+ions together with hydrogen peroxide(H2O2)molecules,which were then transferred into·OH radicals through Fenton-type reactions catalyzed by Cu2+for instant bacteria elimination.At the same time,the released Cu2+and Mg2+ions were retained to improve the angiogenesis and suppress the inflammation infiltration,thus remodeling the wound microenvironment.Meanwhile,the one-dimensional(1D)-constructed nano shish-kebabs and PDA coating on fibers provided additional topological activation for cell adhesion and directed migration along the aligned fiber orientation.Through the meticulous design,the resultant membranes markedly accelerated the infected wound healing in the diabetic rat model.This study pioneers a unique design to develop a nanocomposite fibrous membrane that combines multidimensional topologies with chemodynamic therapy(CDT),for efficiently combating infected diabetic wounds.
文摘The authors regret that in Fig.2Aa of the article,the images for TPUPPy and TPU-aa in the first column were mistakenly duplicated due to a formatting error.The correct version of Fig.2 is provided below,along with the original data for reference.The authors confirm that the rest of the article remains unaffected.
