Cotton is widely distributed worldwide,and improving the quality of its fiber is one of the most important tasks in cotton breeding.Cotton fibers are primarily composed of cellulose,which is synthesized by CesA comple...Cotton is widely distributed worldwide,and improving the quality of its fiber is one of the most important tasks in cotton breeding.Cotton fibers are primarily composed of cellulose,which is synthesized by CesA complexes(CSCs).However,the functions of CesA genes in cotton fiber development have not been comprehensively analysed.In this study,the cotton transcriptome and metabolome were used to investigate the function of CesA genes in fiber development.Finally,321 metabolites were obtained,84 of which were associated with the corresponding genes.Interestingly,a target gene named Gh_A08G144300,one of the CesA gene family members,was closely correlated with the development of cotton fibers.The target CesA gene Gh_A08G144300 was analysed to determine its specific function in cotton fiber development.High-level gene expression of Gh_A08G144300 was found at different fiber development stages by RNA-seq analysis,and the silencing of Gh_A08G144300 visibly inhibited the growth of cotton fibers,showing that it is critical for their growth.This study provides an important reference for research on the gene function of Gh_A08G144300 and the regulatory mechanism of fiber development in cotton.展开更多
Cas1-and-Cas2-mediated new spacer acquisition is an essential process for bacterial adaptive immunity.The process is critical for the ecology of the oral microflora and oral health.Although molecular mechanisms for sp...Cas1-and-Cas2-mediated new spacer acquisition is an essential process for bacterial adaptive immunity.The process is critical for the ecology of the oral microflora and oral health.Although molecular mechanisms for spacer acquisition are known,it has never been established if this process is associated with the morphological changes of bacteria.In this study,we demonstrated a novel Cas2-induced filamentation phenotype in E.coli that was regulated by co-expression of the Cas1 protein.A 30 amino acid motif at the carboxyl terminus of Cas2 is necessary for this function.By imaging analysis,we provided evidence to argue that Cas-induced filamentation is a step coupled with new spacer acquisition during which filaments are characterised by polyploidy with asymmetric cell division.This work may open new opportunities to investigate the adaptive immune response and microbial balance for oral health.展开更多
Rare infectious diseases are infections that are uncommon,have a low incidence,and are caused by newly emerging pathogens,cross‐species or ectopic infections,or host immunodeficiencies.The detection and diagnosis of ...Rare infectious diseases are infections that are uncommon,have a low incidence,and are caused by newly emerging pathogens,cross‐species or ectopic infections,or host immunodeficiencies.The detection and diagnosis of rare infections is one of the main reasons for misdiagnosis and missed diagnosis.Traditional detection techniques such as microbial cultivation and isolation or polymerase chain reaction often fail to meet the clinical demands for timeliness,accuracy,and sensitivity.Metagenomic nextgeneration sequencing involves pan-nucleic acid testing conducted directly using specimens to facilitate rapid identification of rare or unidentified pathogens.Despite the availability of various techniques,advanced methods in clinical practice are necessary to achieve timely clinical diagnosis of rare infections.In this review,we summarize the definition and clinical significance of rare infectious diseases as well as the current detection methods,limitations,and future research areas for their detection.展开更多
基金This project was supported by the Special Fund for Modern Agriculture of Jiangxi Province(JXARS-22)Science and Technology Research Project of Education Department of Jiangxi Province(181366).
文摘Cotton is widely distributed worldwide,and improving the quality of its fiber is one of the most important tasks in cotton breeding.Cotton fibers are primarily composed of cellulose,which is synthesized by CesA complexes(CSCs).However,the functions of CesA genes in cotton fiber development have not been comprehensively analysed.In this study,the cotton transcriptome and metabolome were used to investigate the function of CesA genes in fiber development.Finally,321 metabolites were obtained,84 of which were associated with the corresponding genes.Interestingly,a target gene named Gh_A08G144300,one of the CesA gene family members,was closely correlated with the development of cotton fibers.The target CesA gene Gh_A08G144300 was analysed to determine its specific function in cotton fiber development.High-level gene expression of Gh_A08G144300 was found at different fiber development stages by RNA-seq analysis,and the silencing of Gh_A08G144300 visibly inhibited the growth of cotton fibers,showing that it is critical for their growth.This study provides an important reference for research on the gene function of Gh_A08G144300 and the regulatory mechanism of fiber development in cotton.
基金supported by the National Science and Technology Major Project(2014ZX09101046-004)the National Natural Foundation of China(31600644)
文摘Cas1-and-Cas2-mediated new spacer acquisition is an essential process for bacterial adaptive immunity.The process is critical for the ecology of the oral microflora and oral health.Although molecular mechanisms for spacer acquisition are known,it has never been established if this process is associated with the morphological changes of bacteria.In this study,we demonstrated a novel Cas2-induced filamentation phenotype in E.coli that was regulated by co-expression of the Cas1 protein.A 30 amino acid motif at the carboxyl terminus of Cas2 is necessary for this function.By imaging analysis,we provided evidence to argue that Cas-induced filamentation is a step coupled with new spacer acquisition during which filaments are characterised by polyploidy with asymmetric cell division.This work may open new opportunities to investigate the adaptive immune response and microbial balance for oral health.
基金supported by Horizontal Project of Xinchang County Hospital of Chinese Medicine(2023‐HT‐394).
文摘Rare infectious diseases are infections that are uncommon,have a low incidence,and are caused by newly emerging pathogens,cross‐species or ectopic infections,or host immunodeficiencies.The detection and diagnosis of rare infections is one of the main reasons for misdiagnosis and missed diagnosis.Traditional detection techniques such as microbial cultivation and isolation or polymerase chain reaction often fail to meet the clinical demands for timeliness,accuracy,and sensitivity.Metagenomic nextgeneration sequencing involves pan-nucleic acid testing conducted directly using specimens to facilitate rapid identification of rare or unidentified pathogens.Despite the availability of various techniques,advanced methods in clinical practice are necessary to achieve timely clinical diagnosis of rare infections.In this review,we summarize the definition and clinical significance of rare infectious diseases as well as the current detection methods,limitations,and future research areas for their detection.
