Soil moisture is essential for plant growth in terrestrial ecosystems.This study investigated the visible-near infrared(Vis-NIR)spectra of three subgroups of purple soils(calcareous,neutral,and acidic)from western Cho...Soil moisture is essential for plant growth in terrestrial ecosystems.This study investigated the visible-near infrared(Vis-NIR)spectra of three subgroups of purple soils(calcareous,neutral,and acidic)from western Chongqing,China,containing different water contents.The relationship between soil moisture and spectral reflectivity(R)was analyzed using four spectral transformations,and estimation models were established for estimating the soil moisture content(SMC)of purple soil based on stepwise multiple linear regression(SMLR)and partial least squares regression(PLSR).We found that soil spectra were similar for different moisture contents,with reflectivity decreasing with increasing moisture content and following the order neutral>calcareous>acidic purple soil(at constant moisture content).Three of the four spectral transformations can highlight spectral sensitivity to SMC and significantly improve the correlation between the reflectance spectra and SMC.SMLR and PLSRmethods provide similar prediction accuracy.The PLSR-based model using a first-order reflectivity differential(R?)is more effective for estimating the SMC,and gave coefficient of determination(v2),root mean square errors of validation(RMSEV),and ratio of performance to inter-quartile distance(RPIQ)values of 0.946,1.347,and 6.328,respectively,for the calcareous purple soil,and 0.944,1.818,and 6.569,respectively,for the acidic purple soil.For neutral purple soil,the best prediction was obtained using the SMLR method with R?transformation,yieldingv2,RMSEV and RPIQ values of 0.973,0.888 and 8.791,respectively.In general,PLSR is more suitable than SMLR for estimating the SMC of purple soil.展开更多
腰痛(low back pain,LBP)是影响人类健康的最常见疾病之一,患病率高且治愈率低下。其发病机制尚不清楚,可能与多种因素有关,如椎间盘退变、关节突关节损伤、肌肉筋膜炎症等。建立恰当的动物模型有助于研究和了解LBP的发病机制、探索预...腰痛(low back pain,LBP)是影响人类健康的最常见疾病之一,患病率高且治愈率低下。其发病机制尚不清楚,可能与多种因素有关,如椎间盘退变、关节突关节损伤、肌肉筋膜炎症等。建立恰当的动物模型有助于研究和了解LBP的发病机制、探索预防及治疗方法。本文就可诱发腰痛的动物模型研究进展综述如下。展开更多
目的通过对比细胞凋亡辅助脱细胞法与化学脱细胞法,探究制备马尾神经细胞外基质(ECM)的最佳方案。方法分别采用喜树碱(细胞凋亡辅助脱细胞法)和十二烷基硫酸钠(SDS,化学脱细胞法)制备大鼠马尾神经ECM,采用扫描电子显微镜(SEM)、DNA含量...目的通过对比细胞凋亡辅助脱细胞法与化学脱细胞法,探究制备马尾神经细胞外基质(ECM)的最佳方案。方法分别采用喜树碱(细胞凋亡辅助脱细胞法)和十二烷基硫酸钠(SDS,化学脱细胞法)制备大鼠马尾神经ECM,采用扫描电子显微镜(SEM)、DNA含量测定、组织学等方法评价两种方案的脱细胞效果。分别将大鼠雪旺细胞(SC)及背根神经节(DRG)接种在两种脱细胞方案制备的马尾神经ECM上进行培养,运用免疫细胞化学、细胞增殖实验(CCK-8)、实时荧光定量反转录聚合酶链反应(qRT-PCR)及酶联免疫吸附测定(ELISA)等方法评价两种方案所制备马尾神经ECM的生物相容性、促进SC增殖、髓鞘化、分泌及DRG轴突再生、细胞迁移的能力。结果与化学脱细胞法相比,细胞凋亡辅助脱细胞法制备的马尾神经ECM的DNA残留量更低[48 h DNA残留量(23.898±1.259)ng/mg比(30.200±1.442)ng/mg,P<0.05],基底膜管结构保留更加完整,促BDNF分泌更强[(365.1±12.24)pg/ml比(307.1±16.36)pg/ml,P<0.05],促DRG的轴突再生更长[(1589.1±33.74)μm比(1433.2±37.69)μm,P<0.05],促DRG中的SC迁移更远[迁移距离(1589.1±33.74)μm比(1433.2±37.69)μm,P<0.05]。结论利用细胞凋亡辅助脱细胞法制备马尾神经ECM的效果显著优于化学脱细胞法,该方案将为以马尾神经ECM为基础的组织工程神经移植物的构建提供更为合理有效的脱细胞策略。展开更多
目的探讨髋关节镜手术治疗髋臼盂唇损伤合并囊肿的临床疗效。方法回顾性分析2017年1月至2022年4月于天津市天津医院运动损伤与关节镜科接受髋关节镜手术治疗髋臼盂唇损伤合并囊肿的患者14例,男5例、女9例,年龄(35.46±12.62)岁(范围...目的探讨髋关节镜手术治疗髋臼盂唇损伤合并囊肿的临床疗效。方法回顾性分析2017年1月至2022年4月于天津市天津医院运动损伤与关节镜科接受髋关节镜手术治疗髋臼盂唇损伤合并囊肿的患者14例,男5例、女9例,年龄(35.46±12.62)岁(范围26~57岁)。左髋8例、右髋6例,体质指数(24.35±3.14)kg/m^(2)(范围20.2~28.4 kg/m^(2))。症状持续时间(6.25±4.39)个月(范围3~11个月)。所有患者行髋关节镜下囊肿清理、盂唇缝合术。比较患者手术前后的股骨颈α角、外侧中心边缘角、视觉模拟评分(visual analogue score,VAS)、Harris髋关节评分(Harris hip score,HHS)和国际髋关节结果工具(international hip outcome tool,iHOT-12)评分。结果所有患者均顺利完成手术并获得随访,随访时间为(10.23±2.45)个月(范围1.5~18个月)。患者手术前后髋关节VAS的差异有统计学意义(F=108.47,P<0.001)。术后18个月的VAS为(1.34±0.83)分,小于术前和术后6周的(7.85±1.12)分和(5.03±1.60)分,差异有统计学意义(P<0.05)。患者手术前后髋关节HHS评分的差异有统计学意义(F=96.89,P<0.001)。术后18个月的HHS评分为(85.58±4.65)分,大于术前和术后6周的(54.36±2.31)分和(61.12±1.20)分,差异有统计学意义(P<0.05)。患者手术前后iHOT-12评分的差异有统计学意义(F=117.92,P<0.001)。术后18个月的iHOT-12评分为(78.36±2.28)分,大于术前和术后6周的(31.39±5.21)分和(47.88±2.20)分,差异有统计学意义(P<0.05)。患者手术前后股骨颈α角的差异有统计学意义(F=101.56,P<0.001)。术后12个月的股骨颈α角为45.32°±3.16°,小于术前的50.86°±8.41°,差异有统计学意义(P<0.05)。患者手术前后外侧中心边缘角的差异有统计学意义(F=100.38,P<0.001)。术后12个月的外侧中心边缘角为28.23°±5.32°,小于术前的32.16°±5.13°,差异有统计学意义(P<0.001)。结论髋关节镜手术治疗髋臼盂唇损伤合并囊肿可改善髋部疼痛和提高髋关节功能。展开更多
基金funded by Chongqing Talent Program(CQYC201905009)Chongqing Education Commission(KJZD-K201800502,KJQN201800531)Science Fund for Distinguished Young Scholars of Chongqing(cstc2019jcyjjq X0025)。
文摘Soil moisture is essential for plant growth in terrestrial ecosystems.This study investigated the visible-near infrared(Vis-NIR)spectra of three subgroups of purple soils(calcareous,neutral,and acidic)from western Chongqing,China,containing different water contents.The relationship between soil moisture and spectral reflectivity(R)was analyzed using four spectral transformations,and estimation models were established for estimating the soil moisture content(SMC)of purple soil based on stepwise multiple linear regression(SMLR)and partial least squares regression(PLSR).We found that soil spectra were similar for different moisture contents,with reflectivity decreasing with increasing moisture content and following the order neutral>calcareous>acidic purple soil(at constant moisture content).Three of the four spectral transformations can highlight spectral sensitivity to SMC and significantly improve the correlation between the reflectance spectra and SMC.SMLR and PLSRmethods provide similar prediction accuracy.The PLSR-based model using a first-order reflectivity differential(R?)is more effective for estimating the SMC,and gave coefficient of determination(v2),root mean square errors of validation(RMSEV),and ratio of performance to inter-quartile distance(RPIQ)values of 0.946,1.347,and 6.328,respectively,for the calcareous purple soil,and 0.944,1.818,and 6.569,respectively,for the acidic purple soil.For neutral purple soil,the best prediction was obtained using the SMLR method with R?transformation,yieldingv2,RMSEV and RPIQ values of 0.973,0.888 and 8.791,respectively.In general,PLSR is more suitable than SMLR for estimating the SMC of purple soil.
文摘腰痛(low back pain,LBP)是影响人类健康的最常见疾病之一,患病率高且治愈率低下。其发病机制尚不清楚,可能与多种因素有关,如椎间盘退变、关节突关节损伤、肌肉筋膜炎症等。建立恰当的动物模型有助于研究和了解LBP的发病机制、探索预防及治疗方法。本文就可诱发腰痛的动物模型研究进展综述如下。
文摘目的通过对比细胞凋亡辅助脱细胞法与化学脱细胞法,探究制备马尾神经细胞外基质(ECM)的最佳方案。方法分别采用喜树碱(细胞凋亡辅助脱细胞法)和十二烷基硫酸钠(SDS,化学脱细胞法)制备大鼠马尾神经ECM,采用扫描电子显微镜(SEM)、DNA含量测定、组织学等方法评价两种方案的脱细胞效果。分别将大鼠雪旺细胞(SC)及背根神经节(DRG)接种在两种脱细胞方案制备的马尾神经ECM上进行培养,运用免疫细胞化学、细胞增殖实验(CCK-8)、实时荧光定量反转录聚合酶链反应(qRT-PCR)及酶联免疫吸附测定(ELISA)等方法评价两种方案所制备马尾神经ECM的生物相容性、促进SC增殖、髓鞘化、分泌及DRG轴突再生、细胞迁移的能力。结果与化学脱细胞法相比,细胞凋亡辅助脱细胞法制备的马尾神经ECM的DNA残留量更低[48 h DNA残留量(23.898±1.259)ng/mg比(30.200±1.442)ng/mg,P<0.05],基底膜管结构保留更加完整,促BDNF分泌更强[(365.1±12.24)pg/ml比(307.1±16.36)pg/ml,P<0.05],促DRG的轴突再生更长[(1589.1±33.74)μm比(1433.2±37.69)μm,P<0.05],促DRG中的SC迁移更远[迁移距离(1589.1±33.74)μm比(1433.2±37.69)μm,P<0.05]。结论利用细胞凋亡辅助脱细胞法制备马尾神经ECM的效果显著优于化学脱细胞法,该方案将为以马尾神经ECM为基础的组织工程神经移植物的构建提供更为合理有效的脱细胞策略。
文摘目的探讨髋关节镜手术治疗髋臼盂唇损伤合并囊肿的临床疗效。方法回顾性分析2017年1月至2022年4月于天津市天津医院运动损伤与关节镜科接受髋关节镜手术治疗髋臼盂唇损伤合并囊肿的患者14例,男5例、女9例,年龄(35.46±12.62)岁(范围26~57岁)。左髋8例、右髋6例,体质指数(24.35±3.14)kg/m^(2)(范围20.2~28.4 kg/m^(2))。症状持续时间(6.25±4.39)个月(范围3~11个月)。所有患者行髋关节镜下囊肿清理、盂唇缝合术。比较患者手术前后的股骨颈α角、外侧中心边缘角、视觉模拟评分(visual analogue score,VAS)、Harris髋关节评分(Harris hip score,HHS)和国际髋关节结果工具(international hip outcome tool,iHOT-12)评分。结果所有患者均顺利完成手术并获得随访,随访时间为(10.23±2.45)个月(范围1.5~18个月)。患者手术前后髋关节VAS的差异有统计学意义(F=108.47,P<0.001)。术后18个月的VAS为(1.34±0.83)分,小于术前和术后6周的(7.85±1.12)分和(5.03±1.60)分,差异有统计学意义(P<0.05)。患者手术前后髋关节HHS评分的差异有统计学意义(F=96.89,P<0.001)。术后18个月的HHS评分为(85.58±4.65)分,大于术前和术后6周的(54.36±2.31)分和(61.12±1.20)分,差异有统计学意义(P<0.05)。患者手术前后iHOT-12评分的差异有统计学意义(F=117.92,P<0.001)。术后18个月的iHOT-12评分为(78.36±2.28)分,大于术前和术后6周的(31.39±5.21)分和(47.88±2.20)分,差异有统计学意义(P<0.05)。患者手术前后股骨颈α角的差异有统计学意义(F=101.56,P<0.001)。术后12个月的股骨颈α角为45.32°±3.16°,小于术前的50.86°±8.41°,差异有统计学意义(P<0.05)。患者手术前后外侧中心边缘角的差异有统计学意义(F=100.38,P<0.001)。术后12个月的外侧中心边缘角为28.23°±5.32°,小于术前的32.16°±5.13°,差异有统计学意义(P<0.001)。结论髋关节镜手术治疗髋臼盂唇损伤合并囊肿可改善髋部疼痛和提高髋关节功能。
