数字技术的快速发展正在改变历史园林的保护范式,革新了历史园林的信息获取、处理与表现方法。本文通过回顾与梳理2003年至2024年间中有关历史园林数字化保护研究的中英文文献,分析该领域的研究进展,旨在为当今的历史园林保护利用提供...数字技术的快速发展正在改变历史园林的保护范式,革新了历史园林的信息获取、处理与表现方法。本文通过回顾与梳理2003年至2024年间中有关历史园林数字化保护研究的中英文文献,分析该领域的研究进展,旨在为当今的历史园林保护利用提供参考和思路。本研究运用科学计量分析工具CiteSpace 6.4.R1的可视化功能,对Web of Science核心合集数据库和中国知网数据库中共218篇文献展开研究分析。研究表明:2021年后相关研究增速持续增长;英文文献研究成果主要由国内研究机构产出,占英文发文量的51.42%;统计分析相关文献共获得9个聚类、16个关键词与凸显词;总结发现历史园林数字化保护研究主要分布于历史园林数字化记录、历史园林营造原理分析、历史园林信息传达实践三个领域;研究案例集中于江南私家园林,缺乏岭南园林和近现代园林的研究。研究认为:(1)历史园林数据采集对象应进一步拓展,需补充国内岭南园林与近现代历史园林数据;(2)亟待构建标准统一的地域性历史园林信息平台,服务历史园林管理监测、科研协作与公众展示等多重需求;(3)需进一步推动数字化保护技术、数据成果与生产施工深度融合,助力园林行业数字化转型。展开更多
Transcription factor-mediated cell conversion has been reported in the central nervous system of both rodents and nonhuman primates.In particular,glia-to-neuron conversion has been achieved in the brain and spinal cor...Transcription factor-mediated cell conversion has been reported in the central nervous system of both rodents and nonhuman primates.In particular,glia-to-neuron conversion has been achieved in the brain and spinal cord of animal models for neural regeneration and repair.However,whether glia-to-neuron conversion can be used for brain repair in humans needs to be explored.To investigate the use of glia-to-neuron conversion technology in the human brain,we established a long-term ex vivo culture system using human brain tissue that was surgically removed from epileptic patients to test glia-to-neuron conversion directly.We found that neural transcription factors NeuroD1 and Ascl1 both converted human glial cells into neurons.Immunostaining and electrophysiological recordings showed that the glia-converted neurons demonstrated immature properties during the initial 7-14 days of conversion,and then acquired more mature neuronal properties after 21-27 days of conversion.These ex vivo conversion studies in human brain tissue pave the way toward future clinical trials using a transcription factor-based glia-to-neuron conversion approach to treat neurological disorders.展开更多
Radiation-induced brain injury remains one of the most severe complications of radiotherapy for head and neck tumors,with limited options for prevention and treatment.In situ neural regeneration technology has demonst...Radiation-induced brain injury remains one of the most severe complications of radiotherapy for head and neck tumors,with limited options for prevention and treatment.In situ neural regeneration technology has demonstrated promising therapeutic effects in various neurodegenerative and neurotrauma conditions.In this study,we overexpressed the neural transcription factor NeuroD1 using in situ neural regeneration technology in a radiation-induced brain injury mouse model.This approach converted reactive astrocytes into neurons,increased neuronal density,protected endogenous neurons,decreased microglial activation,reduced peripheral CD8+T cell infiltration,and diminished angiogenesis in the injured area,leading to a significant reduction in lesion volume.Additionally,we explored the potential mechanisms of NeuroD1 in situ neural regeneration technology through bulk RNA sequencing,which showed an upregulation of neurogenesis-related genes and a downregulation of immune response-related and angiogenesis-related genes.Furthermore,our findings suggested that NeuroD1 in situ neural regeneration technology converted reactive astrocytes into neurons and reduced microglial activation in a thalamic hemorrhagic stroke mouse model.In summary,this study supports NeuroD1 in situ neural regeneration technology as a potential therapeutic approach for treating radiation-induced brain injury and hemorrhagic stroke,and offers new insights into the therapeutic role of NeuroD1 in delayed brain injury.展开更多
Dear Editor,We reported a Chinese family carrying a novel Crumbs homologue 1(CRB1)variant(c.1737_1755del).Consanguineous marriage resulted in a homozygous mutation,leading to the onset of Leber congenital amaurosis(LC...Dear Editor,We reported a Chinese family carrying a novel Crumbs homologue 1(CRB1)variant(c.1737_1755del).Consanguineous marriage resulted in a homozygous mutation,leading to the onset of Leber congenital amaurosis(LCA)in their offspring.展开更多
Optic nerve injury leads to axonal degeneration and the death of retinal ganglion cells,which ultimately causes vision loss.Notably,current treatments are limited.In the present study,we explored whether neurogenic di...Optic nerve injury leads to axonal degeneration and the death of retinal ganglion cells,which ultimately causes vision loss.Notably,current treatments are limited.In the present study,we explored whether neurogenic differentiation factor 1(NeuroD1 or ND1)overexpression in retinal Müller cells may repair the retina after optic nerve crush in mice.Adult mice were subjected to optic nerve crush followed by intravitreal AAV-7m8-GFAP-GFP-ND1 virus injection.Immunofluorescent staining,multi-electrode array recording,electroretinogram,and visual behavior tests were then performed to examine retinal and optic nerve structure and retinal function at various post-optic nerve crush and virus injection times.Western blot analysis and quantitative reverse transcription polymerase chain reaction were performed to explore the possible mechanisms.Compared with the control virus,specific overexpression of ND1 in Müller cells greatly improved the light responses of retinal ganglion cells and retinal neurons in optic nerve crush-injured mice as early as 1-2 weeks post-virus injection and lasted for up to 4 weeks.Neuronal survival in the ganglion cell layer and synaptic connections in the inner retina were slightly improved at 2 weeks;however,visual behavior,retinal ganglion cell survival,and optic nerve structure were not improved.ND1 transiently enhanced glial cell-derived neurotrophic factor expression in the optic nerve crush-injured retina but hardly inhibited retinal inflammation within 2 weeks.Together,our data indicate that ND1 overexpression in Müller cells improves retinal function in the optic nerve crush-injured retina,and suggest that its neuroprotective effect may be caused by enhanced glial cell-derived neurotrophic factor release.展开更多
The commercial AM60(Mg−6Al−0.3Mn)die-casting alloy was modified through Mn,Ce,and La micro-alloying,each at a content below 0.2 wt.%.SEM,TEM,and Micro-CT were employed to characterize the microstructures and propertie...The commercial AM60(Mg−6Al−0.3Mn)die-casting alloy was modified through Mn,Ce,and La micro-alloying,each at a content below 0.2 wt.%.SEM,TEM,and Micro-CT were employed to characterize the microstructures and properties of AM60 based alloys.AM60-0.2La alloy showed excellent mechanical properties.The ultimate tensile strength,yield strength,and elongation of(288.0±1.7)MPa,(158.0±1.0)MPa,and(22.0±3.0)%were achieved in AM60-0.2La alloy.Besides,AM60-0.2La alloy exhibited the best corrosion resistance(0.29 mm/a)and fluidity among the investigated four alloys.The excellent mechanical properties and corrosion resistance are mainly attributed to the grain refinement strengthening,low porosity,and low content of large shrinkage porosity,promising for super-sized integrated automotive components.展开更多
智能健康穿戴产品在老年群体中展现出的巨大应用潜力和发展前景,引发学术界广泛研究探讨。为全面剖析国内外老年智能健康穿戴产品领域的研究态势,本文采用文献计量方法,通过共现、聚类及突现分析等多维手段,系统梳理与评估2013—2023年...智能健康穿戴产品在老年群体中展现出的巨大应用潜力和发展前景,引发学术界广泛研究探讨。为全面剖析国内外老年智能健康穿戴产品领域的研究态势,本文采用文献计量方法,通过共现、聚类及突现分析等多维手段,系统梳理与评估2013—2023年中国知网和Web of Science数据库收录的相关文献,借助VOSviewer和CiteSpace进行可视化呈现。结果表明:美国和中国在国际研究中占据主导地位;国内外的研究热点各有侧重,且国外已从技术设备开发与系统集成应用转向探讨老年人对技术的接受与采用;国际研究的知识基础可分为老年技术采纳评估、健康监测技术创新和智能健康管理应用三类。后续应加强工程学和医学多领域的跨学科交叉研究,在健康监测技术与医疗产品融合、用户体验优化及长期效用验证等方面进行深入探讨。展开更多
文摘数字技术的快速发展正在改变历史园林的保护范式,革新了历史园林的信息获取、处理与表现方法。本文通过回顾与梳理2003年至2024年间中有关历史园林数字化保护研究的中英文文献,分析该领域的研究进展,旨在为当今的历史园林保护利用提供参考和思路。本研究运用科学计量分析工具CiteSpace 6.4.R1的可视化功能,对Web of Science核心合集数据库和中国知网数据库中共218篇文献展开研究分析。研究表明:2021年后相关研究增速持续增长;英文文献研究成果主要由国内研究机构产出,占英文发文量的51.42%;统计分析相关文献共获得9个聚类、16个关键词与凸显词;总结发现历史园林数字化保护研究主要分布于历史园林数字化记录、历史园林营造原理分析、历史园林信息传达实践三个领域;研究案例集中于江南私家园林,缺乏岭南园林和近现代园林的研究。研究认为:(1)历史园林数据采集对象应进一步拓展,需补充国内岭南园林与近现代历史园林数据;(2)亟待构建标准统一的地域性历史园林信息平台,服务历史园林管理监测、科研协作与公众展示等多重需求;(3)需进一步推动数字化保护技术、数据成果与生产施工深度融合,助力园林行业数字化转型。
基金supported by the Key Project of Guangzhou City,No.202206060002(to GC)the Guangdong Province Science and Technology Project of China,No.2018B030332001(to GC)+1 种基金the Natural Science Foundation of Guangdong Province of China,No.2020A1515010854(to QW)the Yi-Liang Liu Endowment Fund from Jinan University Education Development Foundation。
文摘Transcription factor-mediated cell conversion has been reported in the central nervous system of both rodents and nonhuman primates.In particular,glia-to-neuron conversion has been achieved in the brain and spinal cord of animal models for neural regeneration and repair.However,whether glia-to-neuron conversion can be used for brain repair in humans needs to be explored.To investigate the use of glia-to-neuron conversion technology in the human brain,we established a long-term ex vivo culture system using human brain tissue that was surgically removed from epileptic patients to test glia-to-neuron conversion directly.We found that neural transcription factors NeuroD1 and Ascl1 both converted human glial cells into neurons.Immunostaining and electrophysiological recordings showed that the glia-converted neurons demonstrated immature properties during the initial 7-14 days of conversion,and then acquired more mature neuronal properties after 21-27 days of conversion.These ex vivo conversion studies in human brain tissue pave the way toward future clinical trials using a transcription factor-based glia-to-neuron conversion approach to treat neurological disorders.
基金the National Natural Science Foundation of China,Nos.81925031(to YT)82330099(to YT)+7 种基金82404189(to KZ)the Key-Area Research and Development Program of Guangdong Province,No.2023B0303040003(to YT)STI 2030-Major Projects,No.2022ZD0211603(to YT)Guangzhou Key Projects of Brain Science and Brain-Like Intelligence Technology,No.202206060002(to GC and YS)Science and Technology Project of Guangdong Province,No.2018B030332001(to GC)Guangdong Provincial Pearl River Project,No.2021ZT09Y552(to GC)the Guangdong Basic and Applied Basic Research Foundation,No.2022A1515110189(to KZ)Sun Yat-sen Pilot Scientific Research Fund,No.YXQH202427(to KZ).
文摘Radiation-induced brain injury remains one of the most severe complications of radiotherapy for head and neck tumors,with limited options for prevention and treatment.In situ neural regeneration technology has demonstrated promising therapeutic effects in various neurodegenerative and neurotrauma conditions.In this study,we overexpressed the neural transcription factor NeuroD1 using in situ neural regeneration technology in a radiation-induced brain injury mouse model.This approach converted reactive astrocytes into neurons,increased neuronal density,protected endogenous neurons,decreased microglial activation,reduced peripheral CD8+T cell infiltration,and diminished angiogenesis in the injured area,leading to a significant reduction in lesion volume.Additionally,we explored the potential mechanisms of NeuroD1 in situ neural regeneration technology through bulk RNA sequencing,which showed an upregulation of neurogenesis-related genes and a downregulation of immune response-related and angiogenesis-related genes.Furthermore,our findings suggested that NeuroD1 in situ neural regeneration technology converted reactive astrocytes into neurons and reduced microglial activation in a thalamic hemorrhagic stroke mouse model.In summary,this study supports NeuroD1 in situ neural regeneration technology as a potential therapeutic approach for treating radiation-induced brain injury and hemorrhagic stroke,and offers new insights into the therapeutic role of NeuroD1 in delayed brain injury.
基金Supported by the National Natural Science Foundation of China(No.81970804)Natural Science Foundation of Hunan Province(No.2021JJ30949).
文摘Dear Editor,We reported a Chinese family carrying a novel Crumbs homologue 1(CRB1)variant(c.1737_1755del).Consanguineous marriage resulted in a homozygous mutation,leading to the onset of Leber congenital amaurosis(LCA)in their offspring.
基金supported by the Guangdong Grant Key Technologies for Treatment of Brain Disorders,China,No.2018B030332001(to GC)the Natural Science Foundation of Guangdong Province,China,No.2023A1515012397(to YX).
文摘Optic nerve injury leads to axonal degeneration and the death of retinal ganglion cells,which ultimately causes vision loss.Notably,current treatments are limited.In the present study,we explored whether neurogenic differentiation factor 1(NeuroD1 or ND1)overexpression in retinal Müller cells may repair the retina after optic nerve crush in mice.Adult mice were subjected to optic nerve crush followed by intravitreal AAV-7m8-GFAP-GFP-ND1 virus injection.Immunofluorescent staining,multi-electrode array recording,electroretinogram,and visual behavior tests were then performed to examine retinal and optic nerve structure and retinal function at various post-optic nerve crush and virus injection times.Western blot analysis and quantitative reverse transcription polymerase chain reaction were performed to explore the possible mechanisms.Compared with the control virus,specific overexpression of ND1 in Müller cells greatly improved the light responses of retinal ganglion cells and retinal neurons in optic nerve crush-injured mice as early as 1-2 weeks post-virus injection and lasted for up to 4 weeks.Neuronal survival in the ganglion cell layer and synaptic connections in the inner retina were slightly improved at 2 weeks;however,visual behavior,retinal ganglion cell survival,and optic nerve structure were not improved.ND1 transiently enhanced glial cell-derived neurotrophic factor expression in the optic nerve crush-injured retina but hardly inhibited retinal inflammation within 2 weeks.Together,our data indicate that ND1 overexpression in Müller cells improves retinal function in the optic nerve crush-injured retina,and suggest that its neuroprotective effect may be caused by enhanced glial cell-derived neurotrophic factor release.
基金financially supported by the National Key Research and Development Program of China(Nos.2022YFB3709300,2021YFB3701000)the National Natural Science Foundation of China(Nos.52271090,52071036,U2037601,U21A2048)+1 种基金Chongqing Science and Technology Commission,China(Nos.CSTB2022TIAD-KPX0021,CSTC2024YCJHBGZXM0164,CSTB2024TIAD-KPX0001)the Fundamental Research Funds for the Central Universities,China(No.2022CDJDX-002)。
文摘The commercial AM60(Mg−6Al−0.3Mn)die-casting alloy was modified through Mn,Ce,and La micro-alloying,each at a content below 0.2 wt.%.SEM,TEM,and Micro-CT were employed to characterize the microstructures and properties of AM60 based alloys.AM60-0.2La alloy showed excellent mechanical properties.The ultimate tensile strength,yield strength,and elongation of(288.0±1.7)MPa,(158.0±1.0)MPa,and(22.0±3.0)%were achieved in AM60-0.2La alloy.Besides,AM60-0.2La alloy exhibited the best corrosion resistance(0.29 mm/a)and fluidity among the investigated four alloys.The excellent mechanical properties and corrosion resistance are mainly attributed to the grain refinement strengthening,low porosity,and low content of large shrinkage porosity,promising for super-sized integrated automotive components.
文摘智能健康穿戴产品在老年群体中展现出的巨大应用潜力和发展前景,引发学术界广泛研究探讨。为全面剖析国内外老年智能健康穿戴产品领域的研究态势,本文采用文献计量方法,通过共现、聚类及突现分析等多维手段,系统梳理与评估2013—2023年中国知网和Web of Science数据库收录的相关文献,借助VOSviewer和CiteSpace进行可视化呈现。结果表明:美国和中国在国际研究中占据主导地位;国内外的研究热点各有侧重,且国外已从技术设备开发与系统集成应用转向探讨老年人对技术的接受与采用;国际研究的知识基础可分为老年技术采纳评估、健康监测技术创新和智能健康管理应用三类。后续应加强工程学和医学多领域的跨学科交叉研究,在健康监测技术与医疗产品融合、用户体验优化及长期效用验证等方面进行深入探讨。
