目的系统评价吡咯替尼联用卡培他滨(Pyr-Cap)与奈拉替尼联用卡培他滨(Ner-Cap)、拉帕替尼联用卡培他滨(Lap-Cap)、恩美曲妥珠单抗(T-DM1)治疗人表皮生长因子受体2(HER2)阳性晚期乳腺癌的有效性和安全性。方法计算机检索PubMed、EMbase、...目的系统评价吡咯替尼联用卡培他滨(Pyr-Cap)与奈拉替尼联用卡培他滨(Ner-Cap)、拉帕替尼联用卡培他滨(Lap-Cap)、恩美曲妥珠单抗(T-DM1)治疗人表皮生长因子受体2(HER2)阳性晚期乳腺癌的有效性和安全性。方法计算机检索PubMed、EMbase、Cochrane Library、Web of Science、中国知网、万方、维普网等数据库,检索Pyr-Cap、Ner-Cap、Lap-Cap、TDM-1、Cap治疗HER2阳性晚期乳腺癌的随机对照试验(RCT),检索时间自2005年1月1日至2024年12月31日。由2名研究者独立筛选文献、提取资料并评价纳入研究的偏倚风险后,采用R软件进行网状Meta分析。结果共纳入6项RCT研究。按概率累积排序曲线下面积排序,在无进展生存期和客观缓解率方面,Pyr-Cap方案最优;在总生存期方面,T-DM1方案和Pyr-Cap方案排名前两位;安全性方面,Pyr-Cap方案出现了3级及以上不良事件的可能性最高,主要为腹泻,但总体可控。结论当前证据显示,Pyr-Cap方案治疗HER2阳性晚期乳腺癌可能具有较好的有效性和安全性。展开更多
β-Elemene is an effective anti-cancer ingredient extracted from the genus Curcuma(Zingiberaceae familiy).In the present study,we demonstrated thatβ-elemene inhibited the proliferation of colorectal cancer cells and ...β-Elemene is an effective anti-cancer ingredient extracted from the genus Curcuma(Zingiberaceae familiy).In the present study,we demonstrated thatβ-elemene inhibited the proliferation of colorectal cancer cells and induced cell cycle arrest in the G2/M phase.In addition,β-elemene induced nuclear chromatin condensation and cell membrane phosphatidylserine eversion,decreased cell mitochondrial membrane potential,and promoted the cleavage of caspase-3,caspase-9 and PARP proteins,indicating apoptosis in colorectal cancer cells.At the same time,β-elemene induced autophagy response,and the treated cells showed autophagic vesicle bilayer membrane structure,which was accompanied by up-regulation of the expression of LC3B and SQSTM1.Furthermore,β-elemene increased ROS levels in colorectal cancer cells,promoted phosphorylation of AMPK protein,and inhibited mTOR protein phosphorylation.In the experiments in vivo,β-elemene inhibited the tumor size and induced apoptosis and autophagy in nude mice.In summary,β-elemene inhibited the occurrence and development of colon cancer xenografts in nude mice,and significantly induced apoptosis and autophagy in colorectal cancer cells in vitro.These effects were associated with regulation of the ROS/AMPK/mTOR signaling.We offered a molecular basis for the development ofβ-elemene as a promising anti-tumor drug candidate for colorectal cancer.展开更多
文摘目的系统评价吡咯替尼联用卡培他滨(Pyr-Cap)与奈拉替尼联用卡培他滨(Ner-Cap)、拉帕替尼联用卡培他滨(Lap-Cap)、恩美曲妥珠单抗(T-DM1)治疗人表皮生长因子受体2(HER2)阳性晚期乳腺癌的有效性和安全性。方法计算机检索PubMed、EMbase、Cochrane Library、Web of Science、中国知网、万方、维普网等数据库,检索Pyr-Cap、Ner-Cap、Lap-Cap、TDM-1、Cap治疗HER2阳性晚期乳腺癌的随机对照试验(RCT),检索时间自2005年1月1日至2024年12月31日。由2名研究者独立筛选文献、提取资料并评价纳入研究的偏倚风险后,采用R软件进行网状Meta分析。结果共纳入6项RCT研究。按概率累积排序曲线下面积排序,在无进展生存期和客观缓解率方面,Pyr-Cap方案最优;在总生存期方面,T-DM1方案和Pyr-Cap方案排名前两位;安全性方面,Pyr-Cap方案出现了3级及以上不良事件的可能性最高,主要为腹泻,但总体可控。结论当前证据显示,Pyr-Cap方案治疗HER2阳性晚期乳腺癌可能具有较好的有效性和安全性。
基金the National Natural Science Foundation of China(Nos.81603339 and 81803774)Anhui Key Research and Development Program(No.201904a-07020092)+1 种基金the Fundamental Research Funds for the Central Universities(Nos.WK9110000016 and WK9110000079)China Postdoctoral Science Foundation(No.2019M662207).
文摘β-Elemene is an effective anti-cancer ingredient extracted from the genus Curcuma(Zingiberaceae familiy).In the present study,we demonstrated thatβ-elemene inhibited the proliferation of colorectal cancer cells and induced cell cycle arrest in the G2/M phase.In addition,β-elemene induced nuclear chromatin condensation and cell membrane phosphatidylserine eversion,decreased cell mitochondrial membrane potential,and promoted the cleavage of caspase-3,caspase-9 and PARP proteins,indicating apoptosis in colorectal cancer cells.At the same time,β-elemene induced autophagy response,and the treated cells showed autophagic vesicle bilayer membrane structure,which was accompanied by up-regulation of the expression of LC3B and SQSTM1.Furthermore,β-elemene increased ROS levels in colorectal cancer cells,promoted phosphorylation of AMPK protein,and inhibited mTOR protein phosphorylation.In the experiments in vivo,β-elemene inhibited the tumor size and induced apoptosis and autophagy in nude mice.In summary,β-elemene inhibited the occurrence and development of colon cancer xenografts in nude mice,and significantly induced apoptosis and autophagy in colorectal cancer cells in vitro.These effects were associated with regulation of the ROS/AMPK/mTOR signaling.We offered a molecular basis for the development ofβ-elemene as a promising anti-tumor drug candidate for colorectal cancer.
