目的以藏红花悬浮细胞为研究对象,克隆藏红花酸糖基转移酶(crocetin glycosyltransferase)UGTCs4基因,并对其进行生物信息学和表达分析。方法采用同源克隆法和5’RACE(rapid amplification of c DNA ends)技术克隆UGTCs4基因的全长c DN...目的以藏红花悬浮细胞为研究对象,克隆藏红花酸糖基转移酶(crocetin glycosyltransferase)UGTCs4基因,并对其进行生物信息学和表达分析。方法采用同源克隆法和5’RACE(rapid amplification of c DNA ends)技术克隆UGTCs4基因的全长c DNA序列。运用生物信息学方法对该基因进行分析,预测其编码蛋白的结构与功能,并通过半定量RT-PCR(semi-quantitative reverse transcription and polymerase Chain reaction)方法检测不同诱导物条件下的基因表达情况。结果UGTCs4基因的c DNA全长为1380 bp,编码459个氨基酸。分子进化树分析,该糖基转移酶基因与已知的藏红花酸糖基转移酶基因UGTCs2具有相同的糖基化藏红花酸的功能。RT-PCR的结果显示,UGTCs4基因能够响应吲哚乙酸(IAA)、脱落酸(ABA)、赤霉素(GA)、过氧化氢(H2O2)、茉莉酸甲酯(Me JA)多种处理,这些处理均能促使其进行转录。结论首次从藏红花悬浮细胞中分离并报道了UGTCs4的c DNA克隆,并证实UGTCs4对不同诱导子的响应情况,为进一步研究藏红花素的生物合成及表达调控研究奠定了基础。展开更多
The drifting baselines of capillary electrophoresis affect the veracity of analysis greatly. This paper presents Threshold Fitting Technique(TFT) so as to subtract the baselines from the original signals and emendate ...The drifting baselines of capillary electrophoresis affect the veracity of analysis greatly. This paper presents Threshold Fitting Technique(TFT) so as to subtract the baselines from the original signals and emendate the signals. In TFT, wavelet and curve fitting technique are applied synthetically, thresholds are decided by the computer automatically. Many experiments of signal processing indicate that TFT is simple for being used, there are few man-induced factors, and the results are satisfactory. TFT can be applied for noisy signals without any pre-processing.展开更多
With the continuous progress of Chinas society, Chinas medical and health system has entered a new stage of development. Hospitals at all levels of society have ushered in the opportunity for rapid development. The or...With the continuous progress of Chinas society, Chinas medical and health system has entered a new stage of development. Hospitals at all levels of society have ushered in the opportunity for rapid development. The original accounting system in hospitals is already difficult to adapt to the new development situation. It is necessary to vigorously promote the new accounting system in hospitals. This paper analyzes the timing and content changes of the promulgation of the new accounting system, the problems in the implementation of the new accounting system and relevant improvement measures, hoping to provide some help for the implementation of the new accounting system in hospitals in our country.展开更多
The early childhood learning period is the basic stage of education. With the promotion of quality education, the core development goals of early childhood education are being diversified. In recent years, due to the ...The early childhood learning period is the basic stage of education. With the promotion of quality education, the core development goals of early childhood education are being diversified. In recent years, due to the development of pre-school education, many kindergarten teachers colleges have been upgraded to secondary schools or undergraduate colleges. The training objectives and teaching fields of pre-school education have also changed greatly. Many courses have been correspondingly expanded, developed and reformed. However, judging from the current practice of kindergarten teaching, the establishment of professional system of pre-school education should be based on the actual needs of childrens teaching.展开更多
文摘目的以藏红花悬浮细胞为研究对象,克隆藏红花酸糖基转移酶(crocetin glycosyltransferase)UGTCs4基因,并对其进行生物信息学和表达分析。方法采用同源克隆法和5’RACE(rapid amplification of c DNA ends)技术克隆UGTCs4基因的全长c DNA序列。运用生物信息学方法对该基因进行分析,预测其编码蛋白的结构与功能,并通过半定量RT-PCR(semi-quantitative reverse transcription and polymerase Chain reaction)方法检测不同诱导物条件下的基因表达情况。结果UGTCs4基因的c DNA全长为1380 bp,编码459个氨基酸。分子进化树分析,该糖基转移酶基因与已知的藏红花酸糖基转移酶基因UGTCs2具有相同的糖基化藏红花酸的功能。RT-PCR的结果显示,UGTCs4基因能够响应吲哚乙酸(IAA)、脱落酸(ABA)、赤霉素(GA)、过氧化氢(H2O2)、茉莉酸甲酯(Me JA)多种处理,这些处理均能促使其进行转录。结论首次从藏红花悬浮细胞中分离并报道了UGTCs4的c DNA克隆,并证实UGTCs4对不同诱导子的响应情况,为进一步研究藏红花素的生物合成及表达调控研究奠定了基础。
基金Supported by the National Natural Science Foundation of China(No.2 9975 0 33)
文摘The drifting baselines of capillary electrophoresis affect the veracity of analysis greatly. This paper presents Threshold Fitting Technique(TFT) so as to subtract the baselines from the original signals and emendate the signals. In TFT, wavelet and curve fitting technique are applied synthetically, thresholds are decided by the computer automatically. Many experiments of signal processing indicate that TFT is simple for being used, there are few man-induced factors, and the results are satisfactory. TFT can be applied for noisy signals without any pre-processing.
文摘With the continuous progress of Chinas society, Chinas medical and health system has entered a new stage of development. Hospitals at all levels of society have ushered in the opportunity for rapid development. The original accounting system in hospitals is already difficult to adapt to the new development situation. It is necessary to vigorously promote the new accounting system in hospitals. This paper analyzes the timing and content changes of the promulgation of the new accounting system, the problems in the implementation of the new accounting system and relevant improvement measures, hoping to provide some help for the implementation of the new accounting system in hospitals in our country.
文摘The early childhood learning period is the basic stage of education. With the promotion of quality education, the core development goals of early childhood education are being diversified. In recent years, due to the development of pre-school education, many kindergarten teachers colleges have been upgraded to secondary schools or undergraduate colleges. The training objectives and teaching fields of pre-school education have also changed greatly. Many courses have been correspondingly expanded, developed and reformed. However, judging from the current practice of kindergarten teaching, the establishment of professional system of pre-school education should be based on the actual needs of childrens teaching.
