目的:利用网络药理学方法和分子对接探讨调肝益气定悸药对甘松-仙鹤草治疗常见心律失常室性期前收缩(PVCs)的物质基础和作用机制。方法:通过中药系统药理学数据库及分析平台(TCMSP)结合相关文献筛选甘松、仙鹤草的化学成分及其靶点。借...目的:利用网络药理学方法和分子对接探讨调肝益气定悸药对甘松-仙鹤草治疗常见心律失常室性期前收缩(PVCs)的物质基础和作用机制。方法:通过中药系统药理学数据库及分析平台(TCMSP)结合相关文献筛选甘松、仙鹤草的化学成分及其靶点。借助人类基因数据库(GeneCards),在线人类孟德尔遗传数据库(OMIM),DrugBank数据库寻找治疗PVCs的潜在靶点。通过STRING平台进行蛋白质相互作用分析。利用Metascape平台进行基因本体(GO)富集分析与京都基因与基因组百科全书(KEGG)通路富集分析。利用Cytoscape 3.8.0软件,构建甘松-仙鹤草药对化学成分-潜在靶点-信号通路网络。利用AutoDock 4.2.6将主要靶点蛋白与甘松-仙鹤草活性成分进行分子对接。结果:甘松-仙鹤草药对中槲皮素、山柰酚、金合欢素等9种活性成分治疗PVCs的靶点主要涉及肿瘤坏死因子(TNF),丝裂原活化蛋白激酶1(MAPK1),蛋白激酶B1(Akt1)等。其潜在靶点主要富集在癌症通路(pathways in cancer),高级糖基化终末产物/高级糖基化终末产物受体信号通路(AGE/RAGE signaling pathway in diabetic complications)等26条相关信号通路。分子对接结果显示该药对大部分活性成分(92.59%)与主要靶点蛋白TNF,MAPK1,Akt1的结合活性较好。结论:甘松-仙鹤草药对中多种活性成分可通过多靶点、多通路的干预,发挥调节心肌离子通道、抗炎症、抗氧化应激等作用以治疗PVCs,同时还可通过抑制单胺氧化酶活性、抗神经损伤等改善抑郁、焦虑相关症状。为进一步探索其治疗PVCs的物质基础及作用机制提供了研究思路和理论基础。展开更多
目的:系统评价痰热清注射液辅助治疗心力衰竭合并肺部感染的临床疗效及安全性。方法:检索中国知网、中国生物医学文献数据库、中文科技期刊全文数据库、万方数据库、Cochrance Library、Web of Science和Pub Med数据库,检索时间从建库至...目的:系统评价痰热清注射液辅助治疗心力衰竭合并肺部感染的临床疗效及安全性。方法:检索中国知网、中国生物医学文献数据库、中文科技期刊全文数据库、万方数据库、Cochrance Library、Web of Science和Pub Med数据库,检索时间从建库至2021年8月,收集痰热清注射液治疗心力衰竭合并肺部感染的临床随机对照试验,由2位研究员独立筛选录入文献数据,运用Rev Man5.4.1软件进行数据分析。结果:共纳入10篇文献,包含862例心力衰竭合并肺部感染患者,其中试验组有431例,对照组有431例。Meta分析结果显示,试验组与对照组相比,可提高临床总有效率[OR=4.56,95%CI(2.79,7.52),P<0.00001],降低C-型反应性蛋白数值[MD=-7.55,95%CI(-11.40,-3.69),P=0.0001],缩短纠正心衰的时间[OR=-4.04,95%CI(-4.59,-3.49),P<0.00001],减少平均住院的天数[MD=-4.78,95%CI(-6.67,-2.89),P<0.00001],两组不良反应发生率差异无统计学意义(P>0.05)。结论:痰热清注射液辅助治疗心力衰竭合并肺部感染,可提高临床总有效率,在降低C-型反应性蛋白数值、缩短纠正心衰的时间、减少平均住院的天数方面具有一定的优势,不良反应较小。但纳入研究数据总体质量偏低,尚需开展更多高质量的随机对照试验以增加循证依据。展开更多
Objective:To systematically evaluate the clinical efficacy and safety of Tanreqing injection in the treatment of heart failure complicated with pulmonary infection.Methods:The database of CNKI,SinoMed,VIP full text da...Objective:To systematically evaluate the clinical efficacy and safety of Tanreqing injection in the treatment of heart failure complicated with pulmonary infection.Methods:The database of CNKI,SinoMed,VIP full text database,Wanfang database,Cochrance Library,Web of Science and PubMed were searched.The retrieval time was from the inception to August 2021.Clinical randomized controlled trial of Tanreqing injection in the treatment of heart failure complicated with pulmonary infection was collected,and two researchers independently screened the document data.Meta-analysis was performed using RevMan 5.4.1 software.Results:A total of 10 documents were included,including 862 cases of heart failure complicated with pulmonary infection,including 431 cases in the test group,and 431 cases in the control group.The Meta analysis showed that compared to the control group,the test group increased clinical efficiency[OR=4.56,95%CI(2.79,7.52),P<0.00001],reduced the value of C-reactive protein[MD=-7.55,95%CI(-11.40,-3.69),P=0.0001],reduced the time required to correct heart failure[OR=-4.04,95%CI(-4.59,-3.49),P<0.00001],reduced the number of days of the average hospitalization[MD=-4.78,95%CI(-6.67,-2.89),P<0.00001],and there were no statistically significant differences in the incidence of adverse reactions.Conclusion:Tanreqing injection,as an auxiliary treatment for heart failure complicated with pulmonary infection,has significantly effective effect on improving efficiency.Tanreqing injection has a certain advantage in reducing C-creative protein values,shortening the time of correcting heart failure,and reducing the number of days of the average hospitalization,and the adverse reactions are smaller.However,the overall quality of the included studies is low,and more high-quality randomized controlled trials are needed to increase the evidence-based basis.展开更多
A low-voltage-driven digital-droplet-transporting chip with an open structure is designed,fabricated and characterized.The digital microfluidic chip is fabricated by the silicon planar process.Using only a single elec...A low-voltage-driven digital-droplet-transporting chip with an open structure is designed,fabricated and characterized.The digital microfluidic chip is fabricated by the silicon planar process.Using only a single electrode panel,the droplet on the chip can be manipulated by electrostatic force under a dc driving voltage.The actuation principle is proposed and verified by the experiment.The experimental results show that the minimum driving voltage decreases as the thickness of the dielectric layer decreases.The driving voltage for a 3µL deionized(DI)water droplet is reduced to 15 V in air and 13.5 V in oil by employing a thin dielectric layer of 600 nm with a high dielectric constant and a coating hydrophobic layer on the top.The DI water droplets are also demonstrated to be transported in two dimensions smoothly in a programmable manner,and the maximum transport speed reaches 96 mm/s.The droplets of normal saline,a solution of 0.9 wt%NaCl,are also successfully manipulated on the chip.展开更多
文摘目的:利用网络药理学方法和分子对接探讨调肝益气定悸药对甘松-仙鹤草治疗常见心律失常室性期前收缩(PVCs)的物质基础和作用机制。方法:通过中药系统药理学数据库及分析平台(TCMSP)结合相关文献筛选甘松、仙鹤草的化学成分及其靶点。借助人类基因数据库(GeneCards),在线人类孟德尔遗传数据库(OMIM),DrugBank数据库寻找治疗PVCs的潜在靶点。通过STRING平台进行蛋白质相互作用分析。利用Metascape平台进行基因本体(GO)富集分析与京都基因与基因组百科全书(KEGG)通路富集分析。利用Cytoscape 3.8.0软件,构建甘松-仙鹤草药对化学成分-潜在靶点-信号通路网络。利用AutoDock 4.2.6将主要靶点蛋白与甘松-仙鹤草活性成分进行分子对接。结果:甘松-仙鹤草药对中槲皮素、山柰酚、金合欢素等9种活性成分治疗PVCs的靶点主要涉及肿瘤坏死因子(TNF),丝裂原活化蛋白激酶1(MAPK1),蛋白激酶B1(Akt1)等。其潜在靶点主要富集在癌症通路(pathways in cancer),高级糖基化终末产物/高级糖基化终末产物受体信号通路(AGE/RAGE signaling pathway in diabetic complications)等26条相关信号通路。分子对接结果显示该药对大部分活性成分(92.59%)与主要靶点蛋白TNF,MAPK1,Akt1的结合活性较好。结论:甘松-仙鹤草药对中多种活性成分可通过多靶点、多通路的干预,发挥调节心肌离子通道、抗炎症、抗氧化应激等作用以治疗PVCs,同时还可通过抑制单胺氧化酶活性、抗神经损伤等改善抑郁、焦虑相关症状。为进一步探索其治疗PVCs的物质基础及作用机制提供了研究思路和理论基础。
基金National Natural Science Foundation of China(No.81573817)。
文摘Objective:To systematically evaluate the clinical efficacy and safety of Tanreqing injection in the treatment of heart failure complicated with pulmonary infection.Methods:The database of CNKI,SinoMed,VIP full text database,Wanfang database,Cochrance Library,Web of Science and PubMed were searched.The retrieval time was from the inception to August 2021.Clinical randomized controlled trial of Tanreqing injection in the treatment of heart failure complicated with pulmonary infection was collected,and two researchers independently screened the document data.Meta-analysis was performed using RevMan 5.4.1 software.Results:A total of 10 documents were included,including 862 cases of heart failure complicated with pulmonary infection,including 431 cases in the test group,and 431 cases in the control group.The Meta analysis showed that compared to the control group,the test group increased clinical efficiency[OR=4.56,95%CI(2.79,7.52),P<0.00001],reduced the value of C-reactive protein[MD=-7.55,95%CI(-11.40,-3.69),P=0.0001],reduced the time required to correct heart failure[OR=-4.04,95%CI(-4.59,-3.49),P<0.00001],reduced the number of days of the average hospitalization[MD=-4.78,95%CI(-6.67,-2.89),P<0.00001],and there were no statistically significant differences in the incidence of adverse reactions.Conclusion:Tanreqing injection,as an auxiliary treatment for heart failure complicated with pulmonary infection,has significantly effective effect on improving efficiency.Tanreqing injection has a certain advantage in reducing C-creative protein values,shortening the time of correcting heart failure,and reducing the number of days of the average hospitalization,and the adverse reactions are smaller.However,the overall quality of the included studies is low,and more high-quality randomized controlled trials are needed to increase the evidence-based basis.
基金by the National Basic Research Program of China under Grant No 2011CB309501the Fund for Creative Research of NSFC under Grant No 61021064+1 种基金the Key Project of NSFC under Grant No 60936001the National Natural Science Foundation of China under Grant No 60876037.
文摘A low-voltage-driven digital-droplet-transporting chip with an open structure is designed,fabricated and characterized.The digital microfluidic chip is fabricated by the silicon planar process.Using only a single electrode panel,the droplet on the chip can be manipulated by electrostatic force under a dc driving voltage.The actuation principle is proposed and verified by the experiment.The experimental results show that the minimum driving voltage decreases as the thickness of the dielectric layer decreases.The driving voltage for a 3µL deionized(DI)water droplet is reduced to 15 V in air and 13.5 V in oil by employing a thin dielectric layer of 600 nm with a high dielectric constant and a coating hydrophobic layer on the top.The DI water droplets are also demonstrated to be transported in two dimensions smoothly in a programmable manner,and the maximum transport speed reaches 96 mm/s.The droplets of normal saline,a solution of 0.9 wt%NaCl,are also successfully manipulated on the chip.
