In recent years,peanut yield and quality are more seriously affected by pod rot disease in China.However,managing this disease has proven challenging due to the wide host range of its pathogens.In this study,four soil...In recent years,peanut yield and quality are more seriously affected by pod rot disease in China.However,managing this disease has proven challenging due to the wide host range of its pathogens.In this study,four soil samples were collected from fields with pod rot disease in Hebei Province,and 454 pyrosequencing was used to analyze the fungal communities structure within them.All 38490 ITS high-quality sequences were grouped into 1203 operational taxonomic units,the fungal community diversity of four soil samples was evaluated and compared using Shannon index and Simpson index.The results showed that members of Ascomycota were dominant,followed by Basidiomycota.According to the BLAST results at the species level,Guehomyces had the highest abundance,accounting for about 7.27%,followed by Alternaria,Fusarium,and Davidiella.The relative abundance of Fusarium oxysporum isolated from rotting peanuts in soil with peanut rot was higher than that in the control,indicating that Fusarium oxysporum might be one of the main pathogenic fungus of peanut rot in this area.This study delved into the broader fungal community associated with peanut pod rot,providing a theoretical foundation for preventing and treating this disease in agriculture.展开更多
An in-line fiber Fabry-Perot interferometer (FPI) based on the hollow-core photonic crystal fiber (HCPCF) for refractive index (RI) measurement is proposed in this paper. The FPI is formed by splicing both ends ...An in-line fiber Fabry-Perot interferometer (FPI) based on the hollow-core photonic crystal fiber (HCPCF) for refractive index (RI) measurement is proposed in this paper. The FPI is formed by splicing both ends of a short section of the HCPCF to single mode fibers (SMFs) and cleaving the SMF pigtail to a proper length. The RI response of the sensor is analyzed theoretically and demonstrated experimentally. The results show that the FPI sensor has linear response to external RI and good repeatability. The sensitivity calculated from the maximum fringe contrast is -136 dB/RIU. A new spectrum differential integration (SDI) method for signal processing is also presented in this study. In this method, the RI is obtained from the integrated intensity of the absolute difference between the interference spectrum and its smoothed spectrum. The results show that the sensitivity obtained from the integrated intensity is about -1.34× 10^5 dB/RIU. Compared with the maximum fringe contrast method, the new SDI method can provide the higher sensitivity, better linearity, improved reliability, and accuracy, and it's also convenient for automatic and fast signal processing in real-time monitoring of RI.展开更多
基金supported by General project of Shandong Provincial Natural Science Foundation(ZR2020MC103,ZR2021MC040)Agricultural Innovation Project of Shandong Academy of Agricultural Sciences(CXGC2022B06,CXGC2022F33).
文摘In recent years,peanut yield and quality are more seriously affected by pod rot disease in China.However,managing this disease has proven challenging due to the wide host range of its pathogens.In this study,four soil samples were collected from fields with pod rot disease in Hebei Province,and 454 pyrosequencing was used to analyze the fungal communities structure within them.All 38490 ITS high-quality sequences were grouped into 1203 operational taxonomic units,the fungal community diversity of four soil samples was evaluated and compared using Shannon index and Simpson index.The results showed that members of Ascomycota were dominant,followed by Basidiomycota.According to the BLAST results at the species level,Guehomyces had the highest abundance,accounting for about 7.27%,followed by Alternaria,Fusarium,and Davidiella.The relative abundance of Fusarium oxysporum isolated from rotting peanuts in soil with peanut rot was higher than that in the control,indicating that Fusarium oxysporum might be one of the main pathogenic fungus of peanut rot in this area.This study delved into the broader fungal community associated with peanut pod rot,providing a theoretical foundation for preventing and treating this disease in agriculture.
基金This research is supported by the National Natural Science Foundations of China (Grant Nos. 61174018 and 61505097) and Fundamental research funds of Shandong University, China (Grant No.2014YQ009).
文摘An in-line fiber Fabry-Perot interferometer (FPI) based on the hollow-core photonic crystal fiber (HCPCF) for refractive index (RI) measurement is proposed in this paper. The FPI is formed by splicing both ends of a short section of the HCPCF to single mode fibers (SMFs) and cleaving the SMF pigtail to a proper length. The RI response of the sensor is analyzed theoretically and demonstrated experimentally. The results show that the FPI sensor has linear response to external RI and good repeatability. The sensitivity calculated from the maximum fringe contrast is -136 dB/RIU. A new spectrum differential integration (SDI) method for signal processing is also presented in this study. In this method, the RI is obtained from the integrated intensity of the absolute difference between the interference spectrum and its smoothed spectrum. The results show that the sensitivity obtained from the integrated intensity is about -1.34× 10^5 dB/RIU. Compared with the maximum fringe contrast method, the new SDI method can provide the higher sensitivity, better linearity, improved reliability, and accuracy, and it's also convenient for automatic and fast signal processing in real-time monitoring of RI.