The complete 15,831 bp nucleotide sequence of the mitochondrial genome from Elimaea cheni （Phaneropterinae） was determined. The putative initiation codon for cox1 was TTA. The phylogeny of Orthoptera based on differ...The complete 15,831 bp nucleotide sequence of the mitochondrial genome from Elimaea cheni （Phaneropterinae） was determined. The putative initiation codon for cox1 was TTA. The phylogeny of Orthoptera based on different mtDNA datasets were analyzed with maximum likelihood （ML） and Bayesian inference （BI）. When all 37 genes （mtDNA） were analyzed simultaneously, the monophyly of Caelifera and Ensifera were recovered in the context of our taxon sampling. The phylogeny of Orthoptera was largely consistent with previous phylogenetic hypotheses. Rhaphidophoridae to be a sister group of Tettigoniidae, and the relationships among four subfamilies of Tettigoniidae were （Phaneropterinae ＋ （Conocephalinae ＋ （Bradyporinae ＋ Tettigoniinae）））. Pyrgomorphidae was the most basal group of Caelifera. The relationships among six acridid subfamilies were （Oedipodinae ＋ （Acridinae ＋ （Gomphocerinae ＋ （Oxyinae ＋ （Callip- taminae ＋ Cyrtacanthacridinae）））））. However, we did not recover a monophyletic Grylloidea. Myrmecophilidae clustered into one clade with Gryllotalpidae instead of with Gryllidae. ML and BI analyses of all protein coding genes （using all nucleotide sequence data or excluding the third codon position, and amino acid sequences） revealed a topology identical to that of the entire mtDNA genome dataset. However, 22 tRNAs genes excluding the DHU loop and TψC loop （TRNA）, and two rRNA genes （RRNA） perform poorly when analyzed as single dataset. Our results suggest that the best phylogenetic inferences were ML and BI methods based on total mtDNA. Excluding tRNA genes, rRNA genes and the third codon position of protein coding genes from dataset and converting nucleotide sequences to amino acid sequences do not positively affect phylogenetic reconstruction.展开更多
The complete mitochondrial genome （mitogenome） of Gampsocleis gratiosa was determined. The 15,929 bp in the size of G. gratiosa mitogenome contains a typical gene content, base composition, and codon usage found in ...The complete mitochondrial genome （mitogenome） of Gampsocleis gratiosa was determined. The 15,929 bp in the size of G. gratiosa mitogenome contains a typical gene content, base composition, and codon usage found in metazoan. All 13 protein coding genes （PCGs） of the G gratiosa mitogenome start with a typical ATN codon. The usual termination codons （TAA and TAG） were found from 10 PCGs. However, the atp6, had4, and had5 had incomplete termination codon （T）. The anticodons of all tRNAs are identical to those observed in Drosophila yakuba and Locusta migratoria, and can be folded in the form of a typical clover leaf structure except for trnS （AGN）. The secondary structure of trnS （AGN） was drawn according with the Steinberg-Cedergren tertiary structure. The A＋T content （67.4%） of the A＋T-rich region is relatively lower among the mitogenome regions, in contrast, it usually contains the highest A＋T content for most insects. Two isolated sequence repeat regions （202 bp） were found in the A＋T-rich region with mapping and secondary structure information.展开更多
The genus Neolarnaca is firstly recorded to raspy cricket fauna of China. A new species and a new subspecies are described： N. longipenna sp. nov. and N. vera aurelostria subsp, nov. Illustrations of the morphologica...The genus Neolarnaca is firstly recorded to raspy cricket fauna of China. A new species and a new subspecies are described： N. longipenna sp. nov. and N. vera aurelostria subsp, nov. Illustrations of the morphological structures are given and a key to the species of the genus is provided. All specimens observed are deposited in the Museum of Hebei University.展开更多
基金supported by the National Natural Science Foundation of China(No.30670279 and 30670252)Talent Foundation of Hebei University(No.2008-138)
文摘The complete 15,831 bp nucleotide sequence of the mitochondrial genome from Elimaea cheni （Phaneropterinae） was determined. The putative initiation codon for cox1 was TTA. The phylogeny of Orthoptera based on different mtDNA datasets were analyzed with maximum likelihood （ML） and Bayesian inference （BI）. When all 37 genes （mtDNA） were analyzed simultaneously, the monophyly of Caelifera and Ensifera were recovered in the context of our taxon sampling. The phylogeny of Orthoptera was largely consistent with previous phylogenetic hypotheses. Rhaphidophoridae to be a sister group of Tettigoniidae, and the relationships among four subfamilies of Tettigoniidae were （Phaneropterinae ＋ （Conocephalinae ＋ （Bradyporinae ＋ Tettigoniinae）））. Pyrgomorphidae was the most basal group of Caelifera. The relationships among six acridid subfamilies were （Oedipodinae ＋ （Acridinae ＋ （Gomphocerinae ＋ （Oxyinae ＋ （Callip- taminae ＋ Cyrtacanthacridinae）））））. However, we did not recover a monophyletic Grylloidea. Myrmecophilidae clustered into one clade with Gryllotalpidae instead of with Gryllidae. ML and BI analyses of all protein coding genes （using all nucleotide sequence data or excluding the third codon position, and amino acid sequences） revealed a topology identical to that of the entire mtDNA genome dataset. However, 22 tRNAs genes excluding the DHU loop and TψC loop （TRNA）, and two rRNA genes （RRNA） perform poorly when analyzed as single dataset. Our results suggest that the best phylogenetic inferences were ML and BI methods based on total mtDNA. Excluding tRNA genes, rRNA genes and the third codon position of protein coding genes from dataset and converting nucleotide sequences to amino acid sequences do not positively affect phylogenetic reconstruction.
基金supported by the National Natural Science Foundation of China(No.30470238 and 30670279)Innovation Foundation of the Graduate Student Cultivation of the Shaanxi Normal University(No.2006CXB003).
文摘The complete mitochondrial genome （mitogenome） of Gampsocleis gratiosa was determined. The 15,929 bp in the size of G. gratiosa mitogenome contains a typical gene content, base composition, and codon usage found in metazoan. All 13 protein coding genes （PCGs） of the G gratiosa mitogenome start with a typical ATN codon. The usual termination codons （TAA and TAG） were found from 10 PCGs. However, the atp6, had4, and had5 had incomplete termination codon （T）. The anticodons of all tRNAs are identical to those observed in Drosophila yakuba and Locusta migratoria, and can be folded in the form of a typical clover leaf structure except for trnS （AGN）. The secondary structure of trnS （AGN） was drawn according with the Steinberg-Cedergren tertiary structure. The A＋T content （67.4%） of the A＋T-rich region is relatively lower among the mitogenome regions, in contrast, it usually contains the highest A＋T content for most insects. Two isolated sequence repeat regions （202 bp） were found in the A＋T-rich region with mapping and secondary structure information.
基金supported by the National Natural Science Foundation of China(31071955,31372232)the Orthoptera Species File Grants
文摘The genus Neolarnaca is firstly recorded to raspy cricket fauna of China. A new species and a new subspecies are described： N. longipenna sp. nov. and N. vera aurelostria subsp, nov. Illustrations of the morphological structures are given and a key to the species of the genus is provided. All specimens observed are deposited in the Museum of Hebei University.
