Bone repair and regeneration is a complex spatiotemporal process recruiting a variety of cell types,which need to precisely mediated for effective healing post-damage.The concept of osteoimmunology emphasizes the exte...Bone repair and regeneration is a complex spatiotemporal process recruiting a variety of cell types,which need to precisely mediated for effective healing post-damage.The concept of osteoimmunology emphasizes the extensive and intricate crosstalk between the bone and the immune system.Despite the significant advancements in understanding osteoimmunology,the precise role of dendritic cells(DCs)in this field remains under investigation.As key antigen-presenting cells,DCs are critical in orchestrating adaptive immune responses and maintaining tissue homeostasis.Recent researches have further revealed the potential of DCs to influence the development or acceleration of inflammatory and autoimmune bone disease,as well as their interaction with skeletal cells in the context of bone repair and regeneration.展开更多
With the advancement of modern technology and the continuous development of science,research into flapping wing aircraft is becoming increasingly sophisticated.Addressing issues such as the large wingspan and heavy ma...With the advancement of modern technology and the continuous development of science,research into flapping wing aircraft is becoming increasingly sophisticated.Addressing issues such as the large wingspan and heavy mass of existing bionic butterfly aircraft,this paper proposes the design of a lightweight lithium battery power supply,a chip integrated into a small circuit board,and a reference to the natural characteristics of butterfly wings.The wings are simulated using 0.125 mm polyethylene terephthalate(PET)film to replicate their movement.The driving structure employs a double motor and a four-bar mechanism to achieve natural and smooth wing vibrations.The control system features a lightweight motor,battery,and a high-performance low-power microcontroller for precise control.Using 3D printing technology,a lightweight design is realized,successfully simulating the structure and movement characteristics of a specific butterfly,demonstrating the principles of mechatronics.Furthermore,the design process incorporates multidisciplinary knowledge,and a workshop combining competitive discipline events with innovation and entrepreneurship has been established.This initiative fosters the deep integration of innovation and entrepreneurship education with professional training,effectively cultivating application-oriented technical talents.展开更多
Based on research into bionic butterflies for environmental detection and ecological management,a scheme was proposed to develop and manufacture a bionic aircraft with two wings inspired by specific butterfly species....Based on research into bionic butterflies for environmental detection and ecological management,a scheme was proposed to develop and manufacture a bionic aircraft with two wings inspired by specific butterfly species.A flapping-wing aircraft with a simple structure was designed,and its two-wing design was optimized.The research focused on several key areas:the design and optimization of the wings,the development of the transmission mechanism,hardware design and fabrication,and 3D printing for component manufacturing.This resulted in the bionic replication of the wing shape and structure of the Tiger Papilio butterfly.The final bionic butterfly features a wingspan of 29.5 cm and a total weight of 13.8 g.This project integrates mechatronic principles and provides a valuable reference for advancements in the field of bionic butterflies.Future research could explore the aerodynamic characteristics of wings and innovative design approaches in greater depth.展开更多
Considering the substantial role played by dendritic cells(DCs) in the immune system to bridge innate and adaptive immunity,studies on DC-mediated immunity toward biomaterials principally center on their adjuvant effe...Considering the substantial role played by dendritic cells(DCs) in the immune system to bridge innate and adaptive immunity,studies on DC-mediated immunity toward biomaterials principally center on their adjuvant effects in facilitating the adaptive immunity of codelivered antigens. However, the effect of the intrinsic properties of biomaterials on dendritic cells has not been clarified. Recently, researchers have begun to investigate and found that biomaterials that are nonadjuvant could also regulate the immune function of DCs and thus affect subsequent tissue regeneration. In the case of proteins adsorbed onto biomaterial surfaces,their intrinsic properties can direct their orientation and conformation, forming “biomaterial-associated molecular patterns(BAMPs)”. Thus, in this review, we focused on the intrinsic physiochemical properties of biomaterials in the absence of antigens that affect DC immune function and summarized the underlying signaling pathways. Moreover, we preliminarily clarified the specific composition of BAMPs and the interplay between some key molecules and DCs, such as heat shock proteins(HSPs) and high mobility group box 1(HMGB1). This review provides a new direction for future biomaterial design, through which modulation of host immune responses is applicable to tissue engineering and immunotherapy.展开更多
Osseointegration seems to be a foreign body reaction equilibrium due to the complicated interactions between the immune and skeletal systems.The heterogeneity of the osteoimmune microenvironment in the osseointegratio...Osseointegration seems to be a foreign body reaction equilibrium due to the complicated interactions between the immune and skeletal systems.The heterogeneity of the osteoimmune microenvironment in the osseointegration of implant materials remains elusive.Here,a single-cell study involving 40043 cells is conducted,and a total of 10 distinct cell clusters are identified from five different groups.A preliminary description of the osteoimmune microenvironment revealed the diverse cellular heterogeneity and dynamic changes modulated by implant properties.The increased immature neutrophils,Ly6C+CCR2hi monocytes,and S100a8hi macrophages induce an aggressive inflammatory response and eventually lead to the formation of fibrous capsule around the stainless steel implant.The enrichment of mature neutrophils,FcgR1hi and differentiated immunomodulatory macrophages around the titanium implant indicates favorable osseointegration under moderate immune response.Neutrophil-depletion mice are conducted to explore the role of neutrophils in osseointegration.Neutrophils may improve bone formation by enhancing the recruitment of BMSCs via the CXCL12/CXCR3 signal axis.These findings contribute to a better knowledge of osteoimmunology and are valuable for the design and modification of‘osteoimmune-smart’biomaterials in the bone regeneration field.展开更多
基金supported by the“Pioneer and Leading Goose+X”research and development program of Zhejiang Province Science and Technology Department(2024C03193)the National Natural Science Foundation of China(No.82271026)Start-up Fund of Stomatology Hospital,School of Stomatology,Zhejiang University School of Medicine(2023PDF017).
文摘Bone repair and regeneration is a complex spatiotemporal process recruiting a variety of cell types,which need to precisely mediated for effective healing post-damage.The concept of osteoimmunology emphasizes the extensive and intricate crosstalk between the bone and the immune system.Despite the significant advancements in understanding osteoimmunology,the precise role of dendritic cells(DCs)in this field remains under investigation.As key antigen-presenting cells,DCs are critical in orchestrating adaptive immune responses and maintaining tissue homeostasis.Recent researches have further revealed the potential of DCs to influence the development or acceleration of inflammatory and autoimmune bone disease,as well as their interaction with skeletal cells in the context of bone repair and regeneration.
基金Innovation and Entrepreneurship Training Project for College Students in Hunan Province in 2024:Design of Small Bionic Butterfly Machine Under the Background of Innovation and Integration(Project No.S202413809022)2023 Innovation and Entrepreneurship Training Project of Hunan College Students:Tiger Butterfly—Bionic Manufacturing and Morphology Research(Project No.S202313809022)。
文摘With the advancement of modern technology and the continuous development of science,research into flapping wing aircraft is becoming increasingly sophisticated.Addressing issues such as the large wingspan and heavy mass of existing bionic butterfly aircraft,this paper proposes the design of a lightweight lithium battery power supply,a chip integrated into a small circuit board,and a reference to the natural characteristics of butterfly wings.The wings are simulated using 0.125 mm polyethylene terephthalate(PET)film to replicate their movement.The driving structure employs a double motor and a four-bar mechanism to achieve natural and smooth wing vibrations.The control system features a lightweight motor,battery,and a high-performance low-power microcontroller for precise control.Using 3D printing technology,a lightweight design is realized,successfully simulating the structure and movement characteristics of a specific butterfly,demonstrating the principles of mechatronics.Furthermore,the design process incorporates multidisciplinary knowledge,and a workshop combining competitive discipline events with innovation and entrepreneurship has been established.This initiative fosters the deep integration of innovation and entrepreneurship education with professional training,effectively cultivating application-oriented technical talents.
基金2023 Innovation and Entrepreneurship Training Project of Hunan College Students:Tiger Butterfly—Bionic Manufacturing and Morphology Research(Project No.S202313809022)Key Project of Education Reform of Hunan Provincial Department of Education:Research on Disciplinary Integration Education Model under Intelligence+Empowerment—A Case Study of Robotics and Logistics Management Majors(Project No.HNJG-20231561)。
文摘Based on research into bionic butterflies for environmental detection and ecological management,a scheme was proposed to develop and manufacture a bionic aircraft with two wings inspired by specific butterfly species.A flapping-wing aircraft with a simple structure was designed,and its two-wing design was optimized.The research focused on several key areas:the design and optimization of the wings,the development of the transmission mechanism,hardware design and fabrication,and 3D printing for component manufacturing.This resulted in the bionic replication of the wing shape and structure of the Tiger Papilio butterfly.The final bionic butterfly features a wingspan of 29.5 cm and a total weight of 13.8 g.This project integrates mechatronic principles and provides a valuable reference for advancements in the field of bionic butterflies.Future research could explore the aerodynamic characteristics of wings and innovative design approaches in greater depth.
基金supported by the Key Research and Development Program of Science and Technology Department of Zhejiang Province(No.2019C03081)。
文摘Considering the substantial role played by dendritic cells(DCs) in the immune system to bridge innate and adaptive immunity,studies on DC-mediated immunity toward biomaterials principally center on their adjuvant effects in facilitating the adaptive immunity of codelivered antigens. However, the effect of the intrinsic properties of biomaterials on dendritic cells has not been clarified. Recently, researchers have begun to investigate and found that biomaterials that are nonadjuvant could also regulate the immune function of DCs and thus affect subsequent tissue regeneration. In the case of proteins adsorbed onto biomaterial surfaces,their intrinsic properties can direct their orientation and conformation, forming “biomaterial-associated molecular patterns(BAMPs)”. Thus, in this review, we focused on the intrinsic physiochemical properties of biomaterials in the absence of antigens that affect DC immune function and summarized the underlying signaling pathways. Moreover, we preliminarily clarified the specific composition of BAMPs and the interplay between some key molecules and DCs, such as heat shock proteins(HSPs) and high mobility group box 1(HMGB1). This review provides a new direction for future biomaterial design, through which modulation of host immune responses is applicable to tissue engineering and immunotherapy.
基金The study was supported by grants from the National Natural Science Foundation of China(No.82271026)the Key Research and Development Program of Science and Technology Department of Zhejiang Province(No.2019C03081).
文摘Osseointegration seems to be a foreign body reaction equilibrium due to the complicated interactions between the immune and skeletal systems.The heterogeneity of the osteoimmune microenvironment in the osseointegration of implant materials remains elusive.Here,a single-cell study involving 40043 cells is conducted,and a total of 10 distinct cell clusters are identified from five different groups.A preliminary description of the osteoimmune microenvironment revealed the diverse cellular heterogeneity and dynamic changes modulated by implant properties.The increased immature neutrophils,Ly6C+CCR2hi monocytes,and S100a8hi macrophages induce an aggressive inflammatory response and eventually lead to the formation of fibrous capsule around the stainless steel implant.The enrichment of mature neutrophils,FcgR1hi and differentiated immunomodulatory macrophages around the titanium implant indicates favorable osseointegration under moderate immune response.Neutrophil-depletion mice are conducted to explore the role of neutrophils in osseointegration.Neutrophils may improve bone formation by enhancing the recruitment of BMSCs via the CXCL12/CXCR3 signal axis.These findings contribute to a better knowledge of osteoimmunology and are valuable for the design and modification of‘osteoimmune-smart’biomaterials in the bone regeneration field.
