In the context of digitalization,course resources exhibit multimodal characteristics,covering various forms such as text,images,and videos.Course knowledge and learning resources are becoming increasingly diverse,prov...In the context of digitalization,course resources exhibit multimodal characteristics,covering various forms such as text,images,and videos.Course knowledge and learning resources are becoming increasingly diverse,providing favorable conditions for students’in-depth and efficient learning.Against this backdrop,how to scientifically apply emerging technologies to automatically collect,process,and integrate digital learning resources such as voices,videos,and courseware texts,and better innovate the organization and presentation forms of course knowledge has become an important development direction for“artificial intelligence+education.”This article elaborates on the elements and characteristics of knowledge graphs,analyzes the construction steps of knowledge graphs,and explores the construction methods of multimodal course knowledge graphs from aspects such as dataset collection,course knowledge ontology identification,knowledge discovery,and association,providing references for the intelligent application of online open courses.展开更多
Objective： We aimed to analysis the HER2 gene status and its relationship with p21 protein expression in gastric carcinoma. Methods： Fluorescence in situ hybridisation （FISH） and immunohistochemistry （IHC） techn...Objective： We aimed to analysis the HER2 gene status and its relationship with p21 protein expression in gastric carcinoma. Methods： Fluorescence in situ hybridisation （FISH） and immunohistochemistry （IHC） techniques were used to detect HER2 gene status and p53 protein in 59 cases of gastric cancer. Results： FISH detection of HER2 gene amplification rate was 16.9% （10/59）, HER2 gene amplification in 49 cases without copy number gain and gene amplification were a total of 49.2% （29/59）. HER2 protein expression was 42.4% （25/59）, HER2 gene amplification rates in patients with ＋＋＋, ＋＋ HER2 protein expression were 3/3 and 5/8, while in patients with ＋ HER2 protein expression, it was 2/14, there was significant difference （P 0.05）. p21 protein expression rate was 49.2% （29/59）, HER2 gene amplification rates and p21 protein expression had significant difference in tumor invasion depth, lymph node metastasis （P 0.05）; had no statistical significance in histological type, age, gender differences （P 0.05）. Conclusion： HER2 gene amplification rate and gene copy number had positively correlation with p21 protein expression, HER2 gene status and expression of p21 protein combined detection can provide a reference value in gastric cancer metastasis, patient’s condition development and prognosis, it also can guide clinical development of individual treatment.展开更多
Background:Doctoral students have much higher risk of anxiety or depression than general population.Doctoral students worldwide are facing varying degrees of mental health risks.Method:Based on the survey data of 6,81...Background:Doctoral students have much higher risk of anxiety or depression than general population.Doctoral students worldwide are facing varying degrees of mental health risks.Method:Based on the survey data of 6,812 doctoral students worldwide in Nature in 2019,Probit and Logit models are used to explore the correlation between thefit of doctoral education and training process and the mental health of doctoral students.Results:(1)The training environmentfit of doctoral students has a significant positive impact on their mental health.(2)The academic professionfit of doctoral students has a significant positive impact on their mental health.(3)The orga-nizational culturefit of doctoral students has a significant positive impact on their mental health.(4)Thefinancial supportfit of doctoral students has a significant positive impact on their mental health.Conclusion:The higher the degree of doctoral students’training environmentfit,academic professionfit,organizational culturefit,andfinancial supportfit,the lower the possibility of anxiety or depression among doctoral students.The current research results can help reveal extensive factors that affect the mental health of doctoral students,facilitate the planning and development of effective intervention measures by universities,improve thefit of the doctoral education and training process,improve the mental health of doctoral students,and boost academic excellence.展开更多
The chemical oxygen demand (COD) is selected as the index in this paper. The system dynamics method is used to analyze the emission right price of medical sewage, the boundary of the emission right price system is det...The chemical oxygen demand (COD) is selected as the index in this paper. The system dynamics method is used to analyze the emission right price of medical sewage, the boundary of the emission right price system is determined, and the system dynamics model of the initial emission right of medical sewage is constructed, in which the system is divided into water resources subsystem, population subsystem, economic subsystem and social subsystem. It is expected to expand the theory of the system modeling of the initial discharge right of medical sewage, and to provide the basis for the relevant decision of the environmental management authorities.展开更多
Transmembrane alkane monooxygenase(AlkB)-type monooxygenases,especially AlkB2 monooxygenases,are crucial for aerobic degradation of the medium-to-long-chain n-alkanes in hydrocarbon-utilizing microorganisms.In this st...Transmembrane alkane monooxygenase(AlkB)-type monooxygenases,especially AlkB2 monooxygenases,are crucial for aerobic degradation of the medium-to-long-chain n-alkanes in hydrocarbon-utilizing microorganisms.In this study,we identified a GntR/VanR transcription regulator AlkR of Pseudomonas aeruginosa SJTD-1 involved in the negative regulation of AlkB2 and deciphered its nature of DNA binding and ligand release.The deletion of alkR enhanced the transcription levels of the alkB2 gene and the utilization efficiency of the medium-to-long-chain n-alkanes by strain SJTD-1.The dimer of AlkR recognizes and binds to a conserved palindromic motif in the promoter of the alkB2 gene,and structural symmetry is vital for DNA binding and transcription repression.The long-chain fatty acyl coenzyme A compounds can release AlkR and stimulate transcription of alkB2,reflecting the effect of alkane catabolic metabolites.Structural insights unveiled that the arginine residues and scaffold residues of AlkR are critical for DNA binding.Further bioinformatics analysis of AlkR revealed the widespread VanR–AlkB couples distributed in Pseudomonadaceae with high conservation in the sequences of functional genes and intergenic regions,highlighting a conserved regulatory pattern for n-alkane utilization across this family.These findings demonstrate the regulatory mechanism and structural basis of GntR/VanR transcription regulators in modulating n-alkane biodegradation and provide valuable insights in improving the bioremediation efficiency of hydrocarbon pollution.展开更多
基金University-level Scientific Research Project in Natural Sciences“Research on the Retrieval Method of Multimodal First-Class Course Teaching Content Based on Knowledge Graph Collaboration”(GKY-2024KYYBK-31)。
文摘In the context of digitalization,course resources exhibit multimodal characteristics,covering various forms such as text,images,and videos.Course knowledge and learning resources are becoming increasingly diverse,providing favorable conditions for students’in-depth and efficient learning.Against this backdrop,how to scientifically apply emerging technologies to automatically collect,process,and integrate digital learning resources such as voices,videos,and courseware texts,and better innovate the organization and presentation forms of course knowledge has become an important development direction for“artificial intelligence+education.”This article elaborates on the elements and characteristics of knowledge graphs,analyzes the construction steps of knowledge graphs,and explores the construction methods of multimodal course knowledge graphs from aspects such as dataset collection,course knowledge ontology identification,knowledge discovery,and association,providing references for the intelligent application of online open courses.
文摘Objective： We aimed to analysis the HER2 gene status and its relationship with p21 protein expression in gastric carcinoma. Methods： Fluorescence in situ hybridisation （FISH） and immunohistochemistry （IHC） techniques were used to detect HER2 gene status and p53 protein in 59 cases of gastric cancer. Results： FISH detection of HER2 gene amplification rate was 16.9% （10/59）, HER2 gene amplification in 49 cases without copy number gain and gene amplification were a total of 49.2% （29/59）. HER2 protein expression was 42.4% （25/59）, HER2 gene amplification rates in patients with ＋＋＋, ＋＋ HER2 protein expression were 3/3 and 5/8, while in patients with ＋ HER2 protein expression, it was 2/14, there was significant difference （P 0.05）. p21 protein expression rate was 49.2% （29/59）, HER2 gene amplification rates and p21 protein expression had significant difference in tumor invasion depth, lymph node metastasis （P 0.05）; had no statistical significance in histological type, age, gender differences （P 0.05）. Conclusion： HER2 gene amplification rate and gene copy number had positively correlation with p21 protein expression, HER2 gene status and expression of p21 protein combined detection can provide a reference value in gastric cancer metastasis, patient’s condition development and prognosis, it also can guide clinical development of individual treatment.
文摘Background:Doctoral students have much higher risk of anxiety or depression than general population.Doctoral students worldwide are facing varying degrees of mental health risks.Method:Based on the survey data of 6,812 doctoral students worldwide in Nature in 2019,Probit and Logit models are used to explore the correlation between thefit of doctoral education and training process and the mental health of doctoral students.Results:(1)The training environmentfit of doctoral students has a significant positive impact on their mental health.(2)The academic professionfit of doctoral students has a significant positive impact on their mental health.(3)The orga-nizational culturefit of doctoral students has a significant positive impact on their mental health.(4)Thefinancial supportfit of doctoral students has a significant positive impact on their mental health.Conclusion:The higher the degree of doctoral students’training environmentfit,academic professionfit,organizational culturefit,andfinancial supportfit,the lower the possibility of anxiety or depression among doctoral students.The current research results can help reveal extensive factors that affect the mental health of doctoral students,facilitate the planning and development of effective intervention measures by universities,improve thefit of the doctoral education and training process,improve the mental health of doctoral students,and boost academic excellence.
文摘The chemical oxygen demand (COD) is selected as the index in this paper. The system dynamics method is used to analyze the emission right price of medical sewage, the boundary of the emission right price system is determined, and the system dynamics model of the initial emission right of medical sewage is constructed, in which the system is divided into water resources subsystem, population subsystem, economic subsystem and social subsystem. It is expected to expand the theory of the system modeling of the initial discharge right of medical sewage, and to provide the basis for the relevant decision of the environmental management authorities.
基金supported by the National Key Research and Development Program of China(2021YFA0909500)the National Natural Science Foundation of China(32370107 and 32170106)the Natural Science Foundation of Shanghai(23ZR1431300).
文摘Transmembrane alkane monooxygenase(AlkB)-type monooxygenases,especially AlkB2 monooxygenases,are crucial for aerobic degradation of the medium-to-long-chain n-alkanes in hydrocarbon-utilizing microorganisms.In this study,we identified a GntR/VanR transcription regulator AlkR of Pseudomonas aeruginosa SJTD-1 involved in the negative regulation of AlkB2 and deciphered its nature of DNA binding and ligand release.The deletion of alkR enhanced the transcription levels of the alkB2 gene and the utilization efficiency of the medium-to-long-chain n-alkanes by strain SJTD-1.The dimer of AlkR recognizes and binds to a conserved palindromic motif in the promoter of the alkB2 gene,and structural symmetry is vital for DNA binding and transcription repression.The long-chain fatty acyl coenzyme A compounds can release AlkR and stimulate transcription of alkB2,reflecting the effect of alkane catabolic metabolites.Structural insights unveiled that the arginine residues and scaffold residues of AlkR are critical for DNA binding.Further bioinformatics analysis of AlkR revealed the widespread VanR–AlkB couples distributed in Pseudomonadaceae with high conservation in the sequences of functional genes and intergenic regions,highlighting a conserved regulatory pattern for n-alkane utilization across this family.These findings demonstrate the regulatory mechanism and structural basis of GntR/VanR transcription regulators in modulating n-alkane biodegradation and provide valuable insights in improving the bioremediation efficiency of hydrocarbon pollution.
