Photodynamic therapy(PDT) has been widely investigated for cancer therapy. The intracellular accumulation of reactive oxygen species(ROS)-damaged protein facilitates tumor cell apoptosis. However, there is growing evi...Photodynamic therapy(PDT) has been widely investigated for cancer therapy. The intracellular accumulation of reactive oxygen species(ROS)-damaged protein facilitates tumor cell apoptosis. However, there is growing evidence that the ubiquitin-proteasome pathway(UPP) significantly impedes PDT by preventing the enrichment of ROS-damaged proteins in tumor cells. To tackle this challenge, we report a facile dual-drug nanoassembly based on the discovery of an interesting co-assembly of bortezomib(BTZ, a proteasome inhibitor) and pyropheophorbide a(PPa) for proteasome inhibition-mediated PDT sensitization.The precisely engineered nanoassembly with the optimal dose ratio of BTZ and PPa demonstrates multiple advantages, including simple fabrication, high drug co-loading efficiency, flexible dose adjustment,good colloidal stability, long systemic circulation, favorable tumor-specific accumulation, as well as significant enrichment of ROS-damaged proteins in tumor cells. As a result, the cooperative nanoassembly exhibits potent synergistic antitumor activity in vivo. This study provides a novel dual-drug engineering modality for multimodal cancer treatment.展开更多
This Letter demonstrates a novel lateral shear interferometer system for simultaneous measurement of three-dimensional(3D)shape and thickness of transparent objects.Multi-frequency fringe patterns can be created by ti...This Letter demonstrates a novel lateral shear interferometer system for simultaneous measurement of three-dimensional(3D)shape and thickness of transparent objects.Multi-frequency fringe patterns can be created by tilting mirrors at different inclination angles.With a single camera,the multi-frequency fringes are recorded in one image.The phase-shift of the fringes can be generated synchronously only by moving a plane-parallel plate along an in-plane parallel direction.According to the feature of transparent materials,the thickness and 3D shape can be reconstructed simultaneously based on the relationship between the in-plane displacement and their characteristics.The experiment was conducted on a thin transparent film subjected to a shearing force,which verifies the feasibility of the proposed system.展开更多
基金financially supported by the Liaoning Revitalization Talents Program (No. XLYC1907129)the Excellent Youth Science Foundation of Liaoning Province (No. 2020-YQ-06)the China Postdoctoral Science Foundation (No. 2020M670794)。
文摘Photodynamic therapy(PDT) has been widely investigated for cancer therapy. The intracellular accumulation of reactive oxygen species(ROS)-damaged protein facilitates tumor cell apoptosis. However, there is growing evidence that the ubiquitin-proteasome pathway(UPP) significantly impedes PDT by preventing the enrichment of ROS-damaged proteins in tumor cells. To tackle this challenge, we report a facile dual-drug nanoassembly based on the discovery of an interesting co-assembly of bortezomib(BTZ, a proteasome inhibitor) and pyropheophorbide a(PPa) for proteasome inhibition-mediated PDT sensitization.The precisely engineered nanoassembly with the optimal dose ratio of BTZ and PPa demonstrates multiple advantages, including simple fabrication, high drug co-loading efficiency, flexible dose adjustment,good colloidal stability, long systemic circulation, favorable tumor-specific accumulation, as well as significant enrichment of ROS-damaged proteins in tumor cells. As a result, the cooperative nanoassembly exhibits potent synergistic antitumor activity in vivo. This study provides a novel dual-drug engineering modality for multimodal cancer treatment.
基金supported by the National Natural Science Foundation of China(Nos.11672167,11602056,11472081,and 11532005)
文摘This Letter demonstrates a novel lateral shear interferometer system for simultaneous measurement of three-dimensional(3D)shape and thickness of transparent objects.Multi-frequency fringe patterns can be created by tilting mirrors at different inclination angles.With a single camera,the multi-frequency fringes are recorded in one image.The phase-shift of the fringes can be generated synchronously only by moving a plane-parallel plate along an in-plane parallel direction.According to the feature of transparent materials,the thickness and 3D shape can be reconstructed simultaneously based on the relationship between the in-plane displacement and their characteristics.The experiment was conducted on a thin transparent film subjected to a shearing force,which verifies the feasibility of the proposed system.
