Zwitterionic materials are now widely used to fabricate various functionalized surfaces for biomedical applications due to their excellent non-fouling properties.However,a newly-discovered zwitterionic material,cholin...Zwitterionic materials are now widely used to fabricate various functionalized surfaces for biomedical applications due to their excellent non-fouling properties.However,a newly-discovered zwitterionic material,choline phosphate(CP),was reported to be cell-adhesive,which makes it different from traditional non-fouling zwitterionic materials such as sulfobetaine,carboxybetaine and phosphorylcholine(PC).To further investigate the properties of CP,a comparative study was conducted and the widely-reported zwitterionic PC was employed as a control which has the same chemical component but opposite orientation of charged groups with CP.For this purpose,CP and PC-functionalized surfaces were prepared by surface-initiated atom transfer radical polymerization(Si-ATRP),and their non-fouling properties were probed by protein adsorption and eukaryotic cell adhesion measurement.Results showed that CP-functionalized surfaces exhibited almost equivalent amounts of adsorbed proteins to that of PC,but they were more beneficial to cells initial adhesion and further spreading in serum-free medium,indicating that CP had a promising prospect of application in tissue engineering.展开更多
基金support by the National Natural Science Foundation of China(51503126,21534008,51573110)Scientific Research Foundation for Young Teachers of Sichuan University(2015SCU11005)Miaozi Project in Science and Technology Innovation Program of Sichuan Province(2016087).
文摘Zwitterionic materials are now widely used to fabricate various functionalized surfaces for biomedical applications due to their excellent non-fouling properties.However,a newly-discovered zwitterionic material,choline phosphate(CP),was reported to be cell-adhesive,which makes it different from traditional non-fouling zwitterionic materials such as sulfobetaine,carboxybetaine and phosphorylcholine(PC).To further investigate the properties of CP,a comparative study was conducted and the widely-reported zwitterionic PC was employed as a control which has the same chemical component but opposite orientation of charged groups with CP.For this purpose,CP and PC-functionalized surfaces were prepared by surface-initiated atom transfer radical polymerization(Si-ATRP),and their non-fouling properties were probed by protein adsorption and eukaryotic cell adhesion measurement.Results showed that CP-functionalized surfaces exhibited almost equivalent amounts of adsorbed proteins to that of PC,but they were more beneficial to cells initial adhesion and further spreading in serum-free medium,indicating that CP had a promising prospect of application in tissue engineering.
