Nitric oxide(NO)and hydrogen sulfide(H_(2)S)gasotransmitters exhibit potential therapeutic effects in the car-diovascular system.Herein,biomimicking multilayer structures of biological blood vessels,bilayer smalldiame...Nitric oxide(NO)and hydrogen sulfide(H_(2)S)gasotransmitters exhibit potential therapeutic effects in the car-diovascular system.Herein,biomimicking multilayer structures of biological blood vessels,bilayer smalldiameter vascular grafts(SDVGs)with on-demand NO and H_(2)S release capabilities,were designed and fabri-cated.The keratin-based H_(2)S donor(KTC)with good biocompatibility and high stability was first synthesized and then electrospun with poly(L-lactide-co-caprolactone)(PLCL)to be used as the outer layer of grafts.The elec-trospun poly(ε-caprolactone)(PCL)mats were aminolyzed and further chelated with copper(II)ions to construct glutathione peroxidase(GPx)-like structural surfaces for the catalytic generation of NO,which acted as the inner layer of grafts.The on-demand release of NO and H_(2)S selectively and synergistically promoted the proliferation and migration of human umbilical vein endothelial cells(HUVECs)while inhibiting the proliferation and migration of human umbilical artery smooth muscle cells(HUASMCs).Dual releases of NO and H_(2)S gaso-transmitters could enhance their respective production,resulting in enhanced promotion of HUVECs and inhi-bition of HUASMCs owing to their combined actions.In addition,the bilayer grafts were conducive to forming endothelial cell layers under flow shear stress.In rat abdominal aorta replacement models,the grafts remained patency for 6 months.These grafts were capable of facilitating rapid endothelialization and alleviating neo-intimal hyperplasia without obvious injury,inflammation,or thrombosis.More importantly,the grafts were expected to avoid calcification with the degradation of the grafts.Taken together,these bilayer grafts will be greatly promising candidates for SDVGs with rapid endothelialization and anti-calcification properties.展开更多
The authors regret to inform that four images/graphs in Fig.3 and 6 lacked order annotations of A/B/C/D.And the order annotations of cell viability of HUVECs and HUASMCs are opposite in Fig.6.The correct form of the t...The authors regret to inform that four images/graphs in Fig.3 and 6 lacked order annotations of A/B/C/D.And the order annotations of cell viability of HUVECs and HUASMCs are opposite in Fig.6.The correct form of the two figures are as below.The authors apologize for any inconvenience caused and state that this does not change the discussion and the scientific conclusions of the article.展开更多
The authors regret to inform that four images/graphs in Fig.3 and 6 lacked order annotations of A/B/C/D.And the order annotations of cell viability of HUVECs and HUASMCs are opposite in Fig.6.The correct form of the t...The authors regret to inform that four images/graphs in Fig.3 and 6 lacked order annotations of A/B/C/D.And the order annotations of cell viability of HUVECs and HUASMCs are opposite in Fig.6.The correct form of the two figures are as below.The authors apologize for any inconvenience caused and state that this does not change the discussion and the scientific conclusions of the article.展开更多
Background:Small-diameter vascular grafts have become the focus of attention in tissue engineering.Thrombosis and aneurysmal dilatation are the two major complications of the loss of vascular access after surgery.Ther...Background:Small-diameter vascular grafts have become the focus of attention in tissue engineering.Thrombosis and aneurysmal dilatation are the two major complications of the loss of vascular access after surgery.Therefore,we focused on fabricating 3D printed electrospun vascular grafts loaded with tetramethylpyrazine(TMP)to overcome these limitations.Methods:Based on electrospinning and 3D printing,3D-printed electrospun vascular grafts loaded with TMP were fabricated.The inner layer of the graft was composed of electrospun poly(L-lactic-cocaprolactone)(PLCL)nanofibers and the outer layer consisted of 3D printed polycaprolactone(PCL)microfibers.The characterization and mechanical properties were tested.The blood compatibility and in vitro cytocompatibility of the grafts were also evaluated.Additionally,rat abdominal aortas were replaced with these 3D-printed electrospun grafts to evaluate their biosafety.Results:Mechanical tests demonstrated that the addition of PCL microfibers could improve the mechanical properties.In vitro experimental data proved that the introduction of TMP effectively inhibited platelet adhesion.Afterwards,rat abdominal aorta was replaced with 3D-printed electrospun grafts.The 3D-printed electrospun graft loaded with TMP showed good biocompatibility and mechanical strength within 6 months and maintained substantial patency without the occurrence of acute thrombosis.Moreover,no obvious aneurysmal dilatation was observed.Conclusions:The study demonstrated that 3D-printed electrospun vascular grafts loaded with TMP may have the potential for injured vascular healing.展开更多
基金supported by the National Natural Science Fund of China(81873923)Jiangsu Higher Education Institutions(19KJA310001 and PAPD)Jiangsu Collaborative Innovation Center of Biomedical Functional Materials.
文摘Nitric oxide(NO)and hydrogen sulfide(H_(2)S)gasotransmitters exhibit potential therapeutic effects in the car-diovascular system.Herein,biomimicking multilayer structures of biological blood vessels,bilayer smalldiameter vascular grafts(SDVGs)with on-demand NO and H_(2)S release capabilities,were designed and fabri-cated.The keratin-based H_(2)S donor(KTC)with good biocompatibility and high stability was first synthesized and then electrospun with poly(L-lactide-co-caprolactone)(PLCL)to be used as the outer layer of grafts.The elec-trospun poly(ε-caprolactone)(PCL)mats were aminolyzed and further chelated with copper(II)ions to construct glutathione peroxidase(GPx)-like structural surfaces for the catalytic generation of NO,which acted as the inner layer of grafts.The on-demand release of NO and H_(2)S selectively and synergistically promoted the proliferation and migration of human umbilical vein endothelial cells(HUVECs)while inhibiting the proliferation and migration of human umbilical artery smooth muscle cells(HUASMCs).Dual releases of NO and H_(2)S gaso-transmitters could enhance their respective production,resulting in enhanced promotion of HUVECs and inhi-bition of HUASMCs owing to their combined actions.In addition,the bilayer grafts were conducive to forming endothelial cell layers under flow shear stress.In rat abdominal aorta replacement models,the grafts remained patency for 6 months.These grafts were capable of facilitating rapid endothelialization and alleviating neo-intimal hyperplasia without obvious injury,inflammation,or thrombosis.More importantly,the grafts were expected to avoid calcification with the degradation of the grafts.Taken together,these bilayer grafts will be greatly promising candidates for SDVGs with rapid endothelialization and anti-calcification properties.
文摘The authors regret to inform that four images/graphs in Fig.3 and 6 lacked order annotations of A/B/C/D.And the order annotations of cell viability of HUVECs and HUASMCs are opposite in Fig.6.The correct form of the two figures are as below.The authors apologize for any inconvenience caused and state that this does not change the discussion and the scientific conclusions of the article.
文摘The authors regret to inform that four images/graphs in Fig.3 and 6 lacked order annotations of A/B/C/D.And the order annotations of cell viability of HUVECs and HUASMCs are opposite in Fig.6.The correct form of the two figures are as below.The authors apologize for any inconvenience caused and state that this does not change the discussion and the scientific conclusions of the article.
基金supported by the Science and Technology Commission of Shanghai Municipality,China(Nos.20S31900900,20DZ2254900)the Sino German Science Foundation Research Exchange Center,China(M-0263)+3 种基金China Education Association for International Exchange(2022181)supported by the General Project of SHDC(SHDC22021213)Fundamental Research Funds for the Central Universities(No.2232023D-10).This project was also supported by Researchers Supporting Project Number(RSP2024R65)King Saud University,Riyadh,Saudi Arabia.
文摘Background:Small-diameter vascular grafts have become the focus of attention in tissue engineering.Thrombosis and aneurysmal dilatation are the two major complications of the loss of vascular access after surgery.Therefore,we focused on fabricating 3D printed electrospun vascular grafts loaded with tetramethylpyrazine(TMP)to overcome these limitations.Methods:Based on electrospinning and 3D printing,3D-printed electrospun vascular grafts loaded with TMP were fabricated.The inner layer of the graft was composed of electrospun poly(L-lactic-cocaprolactone)(PLCL)nanofibers and the outer layer consisted of 3D printed polycaprolactone(PCL)microfibers.The characterization and mechanical properties were tested.The blood compatibility and in vitro cytocompatibility of the grafts were also evaluated.Additionally,rat abdominal aortas were replaced with these 3D-printed electrospun grafts to evaluate their biosafety.Results:Mechanical tests demonstrated that the addition of PCL microfibers could improve the mechanical properties.In vitro experimental data proved that the introduction of TMP effectively inhibited platelet adhesion.Afterwards,rat abdominal aorta was replaced with 3D-printed electrospun grafts.The 3D-printed electrospun graft loaded with TMP showed good biocompatibility and mechanical strength within 6 months and maintained substantial patency without the occurrence of acute thrombosis.Moreover,no obvious aneurysmal dilatation was observed.Conclusions:The study demonstrated that 3D-printed electrospun vascular grafts loaded with TMP may have the potential for injured vascular healing.
