Objective: To explore the correlation between the genetic polymorphism of vitamin D binding protein gene (GC) and allergic rhinitis in Hainan population. Methods: 94 cases of patients with allergic rhinitis (AR) who v...Objective: To explore the correlation between the genetic polymorphism of vitamin D binding protein gene (GC) and allergic rhinitis in Hainan population. Methods: 94 cases of patients with allergic rhinitis (AR) who visit a hospital between March 2017 and December 2017 and 113 cases of healthy controls specimen (HCs) were chosen for the research. We analyzed the 222016 and rs7041 polymorphism in the vitamin D-binding protein gene (GC) by snapshot method. Four kinds of hereditary models including co-dominant, dominant, recessive and super dominant were used to identify the significant SNPs by the online analysis software SNPStats. The statistical analysis was done by SPSS25.0. Results: As rs7041 polymorphisms loci is mentioned, the frequency of TT, GT and GG genotypes of in CR group and HC group is 40.4% VS 42.5%, 44.7% VS 42.5%, 14.9% VS 15%, respectively. Statistical analysis showed that there was no significant difference between the two groups. Three genotype were detected for rs222016 loci which is AG, GG and AA. The frequency of AG, GG and AA in CR group and HCs group is 44.8% VS 40.7%, 12.8% VS 25.7% and 40.4% VS 33.6%, with a P value of 0.062, which is close to significance. The frequency of allele G for rs222016 in the HC group was significantly higher than that in the CR group. Conclusion: There was no significant correlation between the polymorphism of rs7041 and the incidence of allergic rhinitis in Hainan population, and GG genotype and allele G of rs222016 loci was likely to be a protective factor from allergic rhinitis in Hainan.展开更多
Zuili plum （Prunus salicina L.） trees usually set fruit poorly, although they produce high quality fruit. To elucidate the causes of the poor fruit set, pollen tube growth into pistils and fruit set percentage were ...Zuili plum （Prunus salicina L.） trees usually set fruit poorly, although they produce high quality fruit. To elucidate the causes of the poor fruit set, pollen tube growth into pistils and fruit set percentage were investigated after cross-, self- and open-pollination. Ovule development in Zuili pistils was also investigated. Pollen tube penetration into the ovules via the obturator and micropyle was best when Zuili pistils were pollinated by cv. Black Amber （P. domestica） pollen grains, although cross-pollinations with Hongxinli and Miili （P. salicina） pollen were more effective than self- and open-pollination. The fruit set percentage was also highest in pistils pollinated with Black Amber pollen grains. Morphological observation of Zuili pistils revealed that the trees produce ＂double pistils＂, developing two ovaries from a basal pistil, at a rate as high as 28%. In such abnormal pistils, most ovules were lacking an embryo sac or were entirely degenerated. The percentage of normally developed ovules was 24.3% and 8.9% in normal and double pistils, respectively. From these results, we conclude that the main causes of poor fruit set of Zuili plums are a lack of effective cross-pollination and the production of high percentages of double pistils in which normally developed ovules are scarcely formed.展开更多
基金Hainan Provincial Natural Science Foundation of China.Project No:817323,817145,818QN247.
文摘Objective: To explore the correlation between the genetic polymorphism of vitamin D binding protein gene (GC) and allergic rhinitis in Hainan population. Methods: 94 cases of patients with allergic rhinitis (AR) who visit a hospital between March 2017 and December 2017 and 113 cases of healthy controls specimen (HCs) were chosen for the research. We analyzed the 222016 and rs7041 polymorphism in the vitamin D-binding protein gene (GC) by snapshot method. Four kinds of hereditary models including co-dominant, dominant, recessive and super dominant were used to identify the significant SNPs by the online analysis software SNPStats. The statistical analysis was done by SPSS25.0. Results: As rs7041 polymorphisms loci is mentioned, the frequency of TT, GT and GG genotypes of in CR group and HC group is 40.4% VS 42.5%, 44.7% VS 42.5%, 14.9% VS 15%, respectively. Statistical analysis showed that there was no significant difference between the two groups. Three genotype were detected for rs222016 loci which is AG, GG and AA. The frequency of AG, GG and AA in CR group and HCs group is 44.8% VS 40.7%, 12.8% VS 25.7% and 40.4% VS 33.6%, with a P value of 0.062, which is close to significance. The frequency of allele G for rs222016 in the HC group was significantly higher than that in the CR group. Conclusion: There was no significant correlation between the polymorphism of rs7041 and the incidence of allergic rhinitis in Hainan population, and GG genotype and allele G of rs222016 loci was likely to be a protective factor from allergic rhinitis in Hainan.
文摘Zuili plum （Prunus salicina L.） trees usually set fruit poorly, although they produce high quality fruit. To elucidate the causes of the poor fruit set, pollen tube growth into pistils and fruit set percentage were investigated after cross-, self- and open-pollination. Ovule development in Zuili pistils was also investigated. Pollen tube penetration into the ovules via the obturator and micropyle was best when Zuili pistils were pollinated by cv. Black Amber （P. domestica） pollen grains, although cross-pollinations with Hongxinli and Miili （P. salicina） pollen were more effective than self- and open-pollination. The fruit set percentage was also highest in pistils pollinated with Black Amber pollen grains. Morphological observation of Zuili pistils revealed that the trees produce ＂double pistils＂, developing two ovaries from a basal pistil, at a rate as high as 28%. In such abnormal pistils, most ovules were lacking an embryo sac or were entirely degenerated. The percentage of normally developed ovules was 24.3% and 8.9% in normal and double pistils, respectively. From these results, we conclude that the main causes of poor fruit set of Zuili plums are a lack of effective cross-pollination and the production of high percentages of double pistils in which normally developed ovules are scarcely formed.
