AIM:To clarify the clinicopathologic significance of COX-2 expression in human colorectal cancer.METHODS:A total of 128 surgically resected colorectal cancer specimens were immunohistochemically analyzed with the use ...AIM:To clarify the clinicopathologic significance of COX-2 expression in human colorectal cancer.METHODS:A total of 128 surgically resected colorectal cancer specimens were immunohistochemically analyzed with the use of anti-COX-2,anti-VEGF and anti-MMP-2 antibodies.The relationship between the cyclooxygenase-2 expression in primary lesions of colorectal cancer and clinicopathoiogic parameters was evaluated by chi-square test.RESULTS:Among 128 cases of colorectal cancer,87(67.9%)were positive for cyclooxygenase-2.The expression of cyclooxygenase-2 was significantly correlated with the depth of invasion,stage of disease,and metastasis(lymph node and liver).Patients in T3-T4,stagesⅢ-Ⅳand with metastasis had much higher expression of cyclooxygenase-2 than ones in T1-T2,stagesⅠ-Ⅱand without metastasis(P<0.05).Among 45 cases of colorectal cancer with lymph node metastasis,the COX-2-positive rate was 86.7%(39/45)for primary lesions and diffuse cytoplasmic staining for COX-2 protein was detected in cancer cells in 100%of metastatic lesions of the lymph nodes.VEGF expression was detected in 49 tumors(38.3%),and VEGF expression was closely correlated with COX-2 expression.The positive expression rate of VEGF(81.6%)in the cyclooxygenase-2-positive group was higher than that in the cyclooxygenase-2-negative group(18.4%,P<0.05).MMP-2 expression was detected in 88 tumors(68.8%),and MMP-2 expression was closely correlated with COX-2 expression.The positive expression rate of MMP-2(79.6%)in the positive COX-2 group was higher than that in the negative COX-2 group(20.4%,P<0.05).CONCLUSION:Cyclooxygenase-2 may be associated with tumor progression by modulating the angiogenesis and cancer cell motility and invasive potential in colorectal cancer and it can be used as a possible biomarker.展开更多
基金Supported by Hubei Province Natural Science Foundation,No.2000J054
文摘AIM:To clarify the clinicopathologic significance of COX-2 expression in human colorectal cancer.METHODS:A total of 128 surgically resected colorectal cancer specimens were immunohistochemically analyzed with the use of anti-COX-2,anti-VEGF and anti-MMP-2 antibodies.The relationship between the cyclooxygenase-2 expression in primary lesions of colorectal cancer and clinicopathoiogic parameters was evaluated by chi-square test.RESULTS:Among 128 cases of colorectal cancer,87(67.9%)were positive for cyclooxygenase-2.The expression of cyclooxygenase-2 was significantly correlated with the depth of invasion,stage of disease,and metastasis(lymph node and liver).Patients in T3-T4,stagesⅢ-Ⅳand with metastasis had much higher expression of cyclooxygenase-2 than ones in T1-T2,stagesⅠ-Ⅱand without metastasis(P<0.05).Among 45 cases of colorectal cancer with lymph node metastasis,the COX-2-positive rate was 86.7%(39/45)for primary lesions and diffuse cytoplasmic staining for COX-2 protein was detected in cancer cells in 100%of metastatic lesions of the lymph nodes.VEGF expression was detected in 49 tumors(38.3%),and VEGF expression was closely correlated with COX-2 expression.The positive expression rate of VEGF(81.6%)in the cyclooxygenase-2-positive group was higher than that in the cyclooxygenase-2-negative group(18.4%,P<0.05).MMP-2 expression was detected in 88 tumors(68.8%),and MMP-2 expression was closely correlated with COX-2 expression.The positive expression rate of MMP-2(79.6%)in the positive COX-2 group was higher than that in the negative COX-2 group(20.4%,P<0.05).CONCLUSION:Cyclooxygenase-2 may be associated with tumor progression by modulating the angiogenesis and cancer cell motility and invasive potential in colorectal cancer and it can be used as a possible biomarker.
