One possible application for the excess of glycerol, which is an exceeding byproduct in biodiesel industry, was used as carbon and energy sources for bioproducts synthesis. This work aims to evaluate biosurfactant pro...One possible application for the excess of glycerol, which is an exceeding byproduct in biodiesel industry, was used as carbon and energy sources for bioproducts synthesis. This work aims to evaluate biosurfactant production from glycerol by Pseudomonas aeruginosa EQ 109 isolated from crude oil-contaminated soil. Factorial design 2^3 was utilized to optimize the amount of biosurfactant produced, by using pH （A）, initial biomass concentration （B）, and initial glycerol concentration （C） as independent factors. The experiments were carried out in flasks containing 100 mL of mineral medium. Biosurfactant production was monitored by increase of the emulsification of aviation kerosene （E24） and surface tension reduction （STr）. The results have shown that, at pH = 7.0, in order to increase E24, variables as B and C are the most influential, leading to a maximum value of E24 = 79%, as well as for an increase of GC （GCmax = 49%）. STR was the variable with the best correlation factor for the proposed linear model （R2=0.96） and its maximum value was 48%. Xfwas not significant to the model, although it was influenced by pH and C, with C = 40g/L （Xfmax = 4.56 g/L）.展开更多
Transesterification is the most common production process for biodiesel. From this reaction, a glycerin phase is produced that is impure, thus lowering market value. However, because it is rich in carbon, it is an alt...Transesterification is the most common production process for biodiesel. From this reaction, a glycerin phase is produced that is impure, thus lowering market value. However, because it is rich in carbon, it is an alternative for generating bioproducts with a higher added value through bioconversion by microorganisms. The aim of this study was to screen parameters, such as pH （4, 5, 6, 7 and 8） and the initial glycerol concentration at 30 ± ℃ with agitation at 150 rpm for bioemulsifier and lipid synthesis in a submerged medium by Yarrowia lipolytica IMUFRJ 50678 from crude glycerin. The best conditions for bioemulsifier production were 30 ± ℃ at pH： 6 with 50 g/L of initial substrate, which produced 2.7 g/L of lipids, from which the optimum 300.5 mg/L of triglycerides was produced over 48 h of microorganism growth.展开更多
Technological advances in the past 30 years have boosted the use of PSM （membrane separation processes）, important for its efficiency and flexibility of operation. These processes can be used in many types of separa...Technological advances in the past 30 years have boosted the use of PSM （membrane separation processes）, important for its efficiency and flexibility of operation. These processes can be used in many types of separation, with some advantages over the usual separation processes. NF （nanofiltration） is a membrane separation technique, which has properties intermediate between reverse osmosis and ultrafiltration in terms of separated species, because the average of the pores is in the range of 1/2 to 10 nm, and the separation occurs in function of load and size of the species. Usually removes species in solution with an effective diameter of about 1 nm or larger and multivalent ions to a greater extent than monovalent ions. The objective was to study the formation of biofouling on the surface of commercial nanofiltration membrane （Osmonics/GE） and surface membrane synthesized in our laboratory. The study was conducted in permeation system with filtration cell with tangential displacement of 15 bar for 8 days flow. DBNPA （2,2-dibromo-3-nitrilopropionamide） was used as a biocide agent, and an anti-fouling, in concentrations of 5 and 300 ppm, respectively, added to the water coming from the Beach Sea Galleon, RJ. The results demonstrated that there was no change in the flow and rejection of sulphate ions, even in the presence of anti-fouling. The count of aerobic, anaerobic and BRS （sulfate reducing bacteria） in seawater before and after using the DBNPA showed efficiency in controlling these groups of microorganisms and biofouling microbial consortium consisting of the existing in seawater.展开更多
文摘One possible application for the excess of glycerol, which is an exceeding byproduct in biodiesel industry, was used as carbon and energy sources for bioproducts synthesis. This work aims to evaluate biosurfactant production from glycerol by Pseudomonas aeruginosa EQ 109 isolated from crude oil-contaminated soil. Factorial design 2^3 was utilized to optimize the amount of biosurfactant produced, by using pH （A）, initial biomass concentration （B）, and initial glycerol concentration （C） as independent factors. The experiments were carried out in flasks containing 100 mL of mineral medium. Biosurfactant production was monitored by increase of the emulsification of aviation kerosene （E24） and surface tension reduction （STr）. The results have shown that, at pH = 7.0, in order to increase E24, variables as B and C are the most influential, leading to a maximum value of E24 = 79%, as well as for an increase of GC （GCmax = 49%）. STR was the variable with the best correlation factor for the proposed linear model （R2=0.96） and its maximum value was 48%. Xfwas not significant to the model, although it was influenced by pH and C, with C = 40g/L （Xfmax = 4.56 g/L）.
文摘Transesterification is the most common production process for biodiesel. From this reaction, a glycerin phase is produced that is impure, thus lowering market value. However, because it is rich in carbon, it is an alternative for generating bioproducts with a higher added value through bioconversion by microorganisms. The aim of this study was to screen parameters, such as pH （4, 5, 6, 7 and 8） and the initial glycerol concentration at 30 ± ℃ with agitation at 150 rpm for bioemulsifier and lipid synthesis in a submerged medium by Yarrowia lipolytica IMUFRJ 50678 from crude glycerin. The best conditions for bioemulsifier production were 30 ± ℃ at pH： 6 with 50 g/L of initial substrate, which produced 2.7 g/L of lipids, from which the optimum 300.5 mg/L of triglycerides was produced over 48 h of microorganism growth.
文摘Technological advances in the past 30 years have boosted the use of PSM （membrane separation processes）, important for its efficiency and flexibility of operation. These processes can be used in many types of separation, with some advantages over the usual separation processes. NF （nanofiltration） is a membrane separation technique, which has properties intermediate between reverse osmosis and ultrafiltration in terms of separated species, because the average of the pores is in the range of 1/2 to 10 nm, and the separation occurs in function of load and size of the species. Usually removes species in solution with an effective diameter of about 1 nm or larger and multivalent ions to a greater extent than monovalent ions. The objective was to study the formation of biofouling on the surface of commercial nanofiltration membrane （Osmonics/GE） and surface membrane synthesized in our laboratory. The study was conducted in permeation system with filtration cell with tangential displacement of 15 bar for 8 days flow. DBNPA （2,2-dibromo-3-nitrilopropionamide） was used as a biocide agent, and an anti-fouling, in concentrations of 5 and 300 ppm, respectively, added to the water coming from the Beach Sea Galleon, RJ. The results demonstrated that there was no change in the flow and rejection of sulphate ions, even in the presence of anti-fouling. The count of aerobic, anaerobic and BRS （sulfate reducing bacteria） in seawater before and after using the DBNPA showed efficiency in controlling these groups of microorganisms and biofouling microbial consortium consisting of the existing in seawater.
