Moringa oleifera(MO)is traditionally used to mitigate inflammatory-mediated disorders;however,the influence of ecotypic variation on its anti-inflammatory activity remains poorly understood.In this study,we compared t...Moringa oleifera(MO)is traditionally used to mitigate inflammatory-mediated disorders;however,the influence of ecotypic variation on its anti-inflammatory activity remains poorly understood.In this study,we compared the phytochemical composition and anti-inflammatory activity of ethanolic extracts obtained from fresh and dried leaves of four MO ecotypes(India,Paraguay,Mozambique,and Pakistan),all grown under the same outdoor conditions,as well as two commercial powders(Just Moringa and WISSA),using LPS-stimulated RAW 264.7 macrophages.Extracts from fresh leaves were 19-43%more cytotoxic than those from dried leaves,depending on the ecotype,likely due to higher cyanogenic glycoside content.Extracts from the India and Paraguay ecotypes,characterized by high levels of quercetin derivatives and caffeic acids,as well as Just Moringa,enriched in kaempferol derivatives,significantly inhibited LPS-induced nitric oxide(NO)production(p<0.05).Just Moringa and Paraguay extracts also reduced iNOS gene expression(p<0.05 and p<0.01,respectively),whereas only the Paraguay extract decreased iNOS protein levels(p<0.05).In contrast,quercetin-3-O-glucoside and rutin showed significant effects only at concentrations approximately 100-fold higher than those present in the extracts,indicating that the phytocomplex displays greater bioactivity than individual compounds.Overall,these results demonstrate that ecotypic variation strongly affects the polyphenolic composition and anti-inflammatory properties of MO leaves,highlighting the importance of reporting both origin and phytochemical composition in MO-based products.展开更多
Mesenchymal stem cells(MSCs)are multipotent adult stem cells of mesodermal origin that can be isolated from various tissues,including bone marrow,tooth pulp,adipose tissue,and umbilical cord.MSCs have gained significa...Mesenchymal stem cells(MSCs)are multipotent adult stem cells of mesodermal origin that can be isolated from various tissues,including bone marrow,tooth pulp,adipose tissue,and umbilical cord.MSCs have gained significant attention in regenerative medicine due to their ability to modulate the immune system and favor tissue repair.MSCs enrich the medium in which they are cultivated with a broad range of bioactive molecules,including growth factors,cytokines,chemokines,enzymes,nucleic acids,and extracellular vesicles that collectively compose the MSC secretome.An increasing number of pre-clinical studies suggest that delivering in vivo an MSC-conditioned medium(i.e.,the medium collected from MSC cultures after at least 3 days of exposure)exerts neuroprotective and anti-inflammatory effects in a variety of neurological conditions.展开更多
Slug catcher is an important and costly equipment in the up-stream assets,but is the less supported by research or commercial simulation software in terms of design and sizing criteria.The purpose of this paper is to ...Slug catcher is an important and costly equipment in the up-stream assets,but is the less supported by research or commercial simulation software in terms of design and sizing criteria.The purpose of this paper is to present a simulation model based on the open-source software for computational fluid-dynamics OpenFOAM®,which can assist during the preliminary sizing,made by the usual simplified methods,proving the fluid-dynamic behaviour and drive the process engineers to an optimum final design by checking all the expected production profiles of the related oil/gas field,for a comprehensive and safe operation all along the reservoir life.The slug catcher main function is to ensure the separation of the phases within the acceptance specifications dictated by the process units of downstream CPF(Central Production Facility).The optimization of the slug catcher could allow the reduction of the equipment overall dimensions,that is essential to reduce the capital expenditure and make the installation more flexible.The graphic output is a strong tool in the hand of engineers,following the fluid-dynamic response of the slug catcher in the gas-liquid separation section,in function of the flow pattern at the entrance and superficial velocities of the phases.展开更多
文摘Moringa oleifera(MO)is traditionally used to mitigate inflammatory-mediated disorders;however,the influence of ecotypic variation on its anti-inflammatory activity remains poorly understood.In this study,we compared the phytochemical composition and anti-inflammatory activity of ethanolic extracts obtained from fresh and dried leaves of four MO ecotypes(India,Paraguay,Mozambique,and Pakistan),all grown under the same outdoor conditions,as well as two commercial powders(Just Moringa and WISSA),using LPS-stimulated RAW 264.7 macrophages.Extracts from fresh leaves were 19-43%more cytotoxic than those from dried leaves,depending on the ecotype,likely due to higher cyanogenic glycoside content.Extracts from the India and Paraguay ecotypes,characterized by high levels of quercetin derivatives and caffeic acids,as well as Just Moringa,enriched in kaempferol derivatives,significantly inhibited LPS-induced nitric oxide(NO)production(p<0.05).Just Moringa and Paraguay extracts also reduced iNOS gene expression(p<0.05 and p<0.01,respectively),whereas only the Paraguay extract decreased iNOS protein levels(p<0.05).In contrast,quercetin-3-O-glucoside and rutin showed significant effects only at concentrations approximately 100-fold higher than those present in the extracts,indicating that the phytocomplex displays greater bioactivity than individual compounds.Overall,these results demonstrate that ecotypic variation strongly affects the polyphenolic composition and anti-inflammatory properties of MO leaves,highlighting the importance of reporting both origin and phytochemical composition in MO-based products.
基金supported by the National Centre for the Replacement Refinement & Reduction of Animals in Research Crack It solution “Neuroinflammation and nociception in a dish”(https://nc3rs.org.uk/crackit/news/new-solutionneuroinflammation-and-nociception-dish) fund(to AM)。
文摘Mesenchymal stem cells(MSCs)are multipotent adult stem cells of mesodermal origin that can be isolated from various tissues,including bone marrow,tooth pulp,adipose tissue,and umbilical cord.MSCs have gained significant attention in regenerative medicine due to their ability to modulate the immune system and favor tissue repair.MSCs enrich the medium in which they are cultivated with a broad range of bioactive molecules,including growth factors,cytokines,chemokines,enzymes,nucleic acids,and extracellular vesicles that collectively compose the MSC secretome.An increasing number of pre-clinical studies suggest that delivering in vivo an MSC-conditioned medium(i.e.,the medium collected from MSC cultures after at least 3 days of exposure)exerts neuroprotective and anti-inflammatory effects in a variety of neurological conditions.
文摘Slug catcher is an important and costly equipment in the up-stream assets,but is the less supported by research or commercial simulation software in terms of design and sizing criteria.The purpose of this paper is to present a simulation model based on the open-source software for computational fluid-dynamics OpenFOAM®,which can assist during the preliminary sizing,made by the usual simplified methods,proving the fluid-dynamic behaviour and drive the process engineers to an optimum final design by checking all the expected production profiles of the related oil/gas field,for a comprehensive and safe operation all along the reservoir life.The slug catcher main function is to ensure the separation of the phases within the acceptance specifications dictated by the process units of downstream CPF(Central Production Facility).The optimization of the slug catcher could allow the reduction of the equipment overall dimensions,that is essential to reduce the capital expenditure and make the installation more flexible.The graphic output is a strong tool in the hand of engineers,following the fluid-dynamic response of the slug catcher in the gas-liquid separation section,in function of the flow pattern at the entrance and superficial velocities of the phases.
