Caramel milk confectioneries,such as dulce de leche,are produced by simmering whole or reconstituted milk with sugar and alkali at temperatures ranging from 115 to 130℃for several hours.This process triggers extensiv...Caramel milk confectioneries,such as dulce de leche,are produced by simmering whole or reconstituted milk with sugar and alkali at temperatures ranging from 115 to 130℃for several hours.This process triggers extensive non-enzymatic browning through the Maillard reaction,leading to significant modifications in milk proteins.Our research focused on the effects of such severe processing conditions on the structural integrity and digestibility of milk proteins,using dulce de leche as a model for severely transformed food protein systems.Due to structural modifications,covalent interchain cross-links,and sugar-derived bridging moieties,milk proteins in dulce de leche form high molecular weight aggregates that are not resolvable by SDS-PAGE.Proteomic analysis revealed that all major milk proteins are involved in the formation of these covalent molecular complexes.When subjected to static gastro-duodenal digestion using both adult and infant models,these macroaggregates showed resistance to pepsin and marginal susceptibility to duodenal proteases.High pressure liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)-based peptidomic analysis of dulce de leche digests allowed identifying variously sized polypeptides bearing the hallmarks of the Maillard reaction.However,many MS signals could not be assigned due to unknown modifications.The prevalence of free and peptide-bound advanced glycated end products(AGEs)in caramel milk products and other ultra-processed foods poses significant health concerns,stressing the need for a comprehensive dedicated evaluation.展开更多
The regulatory classification of dairy products has significant implications for international trade and labeling compliance.Mascarpone,a high-fat used in culinary applications,occupies a regulatory grey area.This stu...The regulatory classification of dairy products has significant implications for international trade and labeling compliance.Mascarpone,a high-fat used in culinary applications,occupies a regulatory grey area.This study employed a comprehensive proteomic approach to determine whether mascarpone meets the molecular and compositional criteria for cheese.Proteins from two mascarpone samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE)and analyzed by high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS),targetingκ-casein,its rennet-derived fragment para-κ-casein,and the whey proteins-to-caseins ratio.Intact proteins were also analyzed by HPLC-MS.Our results showed thatκ-casein remained intact,while para-κ-casein was undetectable in mascarpone samples.Furthermore,the high whey proteins-to-casein ratio reflected the absence of a drainage step typical of curd formation.These findings have been further confirmed by analyzing proteins from four additional commercial samples of mascarpone in comparison with mozzarella and primo sale cheese,as examples of rennet-coagulated dairy products.Data provide evidence that mascarpone lacks key biochemical markers of cheesemaking,supporting its classification as a thermally stabilized acidified dairy emulsion rather than a true cheese.This study highlights the usefulness of proteomic markers for the accurate classification of dairy products.展开更多
基金partly funded by Italian Ministry of University and Research through the PRIN(Research Projects of National Relevance)projects 2022LXS875 and 20224M92SCproject“Nutrizione,Alimentazione ed Invecchiamento Attivo(NUTRAGE)"(FOE 2021-2022)funded by the Italian National Research CouncilNational Recovery and Resilience Plan,mission 4,component 2,investment 1.3,call n.341/2022 of Italian Ministry of University and Research funded by the European Union-NextGenerationEU for the project“ON Foods-Research and innovation network on food and nutrition Sustainability,Safety and Security-Working ON Foods",project PE00000003,concession decreen.1550/2022,CUP B83C22004790001.
文摘Caramel milk confectioneries,such as dulce de leche,are produced by simmering whole or reconstituted milk with sugar and alkali at temperatures ranging from 115 to 130℃for several hours.This process triggers extensive non-enzymatic browning through the Maillard reaction,leading to significant modifications in milk proteins.Our research focused on the effects of such severe processing conditions on the structural integrity and digestibility of milk proteins,using dulce de leche as a model for severely transformed food protein systems.Due to structural modifications,covalent interchain cross-links,and sugar-derived bridging moieties,milk proteins in dulce de leche form high molecular weight aggregates that are not resolvable by SDS-PAGE.Proteomic analysis revealed that all major milk proteins are involved in the formation of these covalent molecular complexes.When subjected to static gastro-duodenal digestion using both adult and infant models,these macroaggregates showed resistance to pepsin and marginal susceptibility to duodenal proteases.High pressure liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)-based peptidomic analysis of dulce de leche digests allowed identifying variously sized polypeptides bearing the hallmarks of the Maillard reaction.However,many MS signals could not be assigned due to unknown modifications.The prevalence of free and peptide-bound advanced glycated end products(AGEs)in caramel milk products and other ultra-processed foods poses significant health concerns,stressing the need for a comprehensive dedicated evaluation.
基金financed by the European Union ELIXIRxNextGenEU through the Italian Ministry of University and Research under the project:"Consolidation of the Italian Infrastructure for Omics and Bioinformatics"ElixirxNextGenIT(Investment PNRRM4C2-I3.1,Project IR_0000010,CUP B53C22001800006).
文摘The regulatory classification of dairy products has significant implications for international trade and labeling compliance.Mascarpone,a high-fat used in culinary applications,occupies a regulatory grey area.This study employed a comprehensive proteomic approach to determine whether mascarpone meets the molecular and compositional criteria for cheese.Proteins from two mascarpone samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE)and analyzed by high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS),targetingκ-casein,its rennet-derived fragment para-κ-casein,and the whey proteins-to-caseins ratio.Intact proteins were also analyzed by HPLC-MS.Our results showed thatκ-casein remained intact,while para-κ-casein was undetectable in mascarpone samples.Furthermore,the high whey proteins-to-casein ratio reflected the absence of a drainage step typical of curd formation.These findings have been further confirmed by analyzing proteins from four additional commercial samples of mascarpone in comparison with mozzarella and primo sale cheese,as examples of rennet-coagulated dairy products.Data provide evidence that mascarpone lacks key biochemical markers of cheesemaking,supporting its classification as a thermally stabilized acidified dairy emulsion rather than a true cheese.This study highlights the usefulness of proteomic markers for the accurate classification of dairy products.
