Benign prostatic hyperplasia(BPH)and prostatic cancer are aging-associated urological conditions in men.While significant advances have been made in relation to treatment,poor response,treatment resistance and adverse...Benign prostatic hyperplasia(BPH)and prostatic cancer are aging-associated urological conditions in men.While significant advances have been made in relation to treatment,poor response,treatment resistance and adverse side-effects limit currently available therapies.The possible antiproliferative and/or ameliorative potentials of Juglans regia have been reported,however,there is a dearth of scientific information on its effect on BPH or prostatic cancer.In this study,in-vivo and in-vitro studies were used to assess the possible benefits of fresh walnut fruits extract in BPH or prostate cancer.High-performance liquid chromatography coupled with electro spray ionization and quadrupole-time-of-flight mass spectrometry(HPLC-ESI-Q-TOF)was used to assess the chemical profile of the plant extract.The anti-proliferative activity of Juglans regia extract was tested against normal and cancerous human prostate cell lines by using the iCELLigence real-time and label-free cell analysis system.The ameliorative potential of fresh fruits Juglans regia extract was assessed by administering the extract at 50 and 100 mg/kg body weight to rats with testosterone induced BPH.Analysis of phytochemicals in Juglans regia revealed a high concentration of phenolic acids and flavonoids,Juglans regia also showing time-and dose-dependent anti-proliferative activity against prostate cancer cells,and reversed biochemical and histomorphological changes induced by testosterone-induced BPH.展开更多
基金support provided by CICT of Universidad de Ja´en(UJA,MINECO,Junta de Andalucía,FEDER)is gratefully acknowledgedWe are grateful to PON MUR Ricerca Innovazione 2014-2020 for PhD funds of L.M.
文摘Benign prostatic hyperplasia(BPH)and prostatic cancer are aging-associated urological conditions in men.While significant advances have been made in relation to treatment,poor response,treatment resistance and adverse side-effects limit currently available therapies.The possible antiproliferative and/or ameliorative potentials of Juglans regia have been reported,however,there is a dearth of scientific information on its effect on BPH or prostatic cancer.In this study,in-vivo and in-vitro studies were used to assess the possible benefits of fresh walnut fruits extract in BPH or prostate cancer.High-performance liquid chromatography coupled with electro spray ionization and quadrupole-time-of-flight mass spectrometry(HPLC-ESI-Q-TOF)was used to assess the chemical profile of the plant extract.The anti-proliferative activity of Juglans regia extract was tested against normal and cancerous human prostate cell lines by using the iCELLigence real-time and label-free cell analysis system.The ameliorative potential of fresh fruits Juglans regia extract was assessed by administering the extract at 50 and 100 mg/kg body weight to rats with testosterone induced BPH.Analysis of phytochemicals in Juglans regia revealed a high concentration of phenolic acids and flavonoids,Juglans regia also showing time-and dose-dependent anti-proliferative activity against prostate cancer cells,and reversed biochemical and histomorphological changes induced by testosterone-induced BPH.
