Background:Metabolic regulation is critical during liver regeneration in rodents,but human data are limited.We investigated perioperative dynamics of circulating metabolites and plasma levels of glucagon-like peptide-...Background:Metabolic regulation is critical during liver regeneration in rodents,but human data are limited.We investigated perioperative dynamics of circulating metabolites and plasma levels of glucagon-like peptide-1(GLP-1)and GLP-2,in patients undergoing liver resections,exploring their associations with the histological phenotype of metabolic dysfunction-associated steatotic liver disease(MASLD)and posthepatectomy liver failure(PHLF).Methods:Eighty-one and 75 patients from two centers between 2012 and 2023 were studied.Targeted quantitative metabolomic assay of 180 circulating metabolites,perioperative GLP-1,GLP-2,and standard lipid parameter level evaluation was employed.An exploratory PHLF prediction model was developed,including GLP-1 as a metabolic parameter.Results:Significant alterations of 44 metabolites by postoperative day(POD)1 and 40 by POD5 were observed,mainly among phospholipid species.Unsupervised clustering identified two metabolic clusters,with one encompassing 93%of PHLF patients by POD5(P<0.001).Standard plasma lipid parameters displayed consistent decrease after hepatectomy,independent from MASLD phenotype,with the lowest levels in PHLF patients.Postoperative GLP-1 and GLP-2 dynamics displayed a reciprocal pattern,indicating adaptive change in secretion.Preoperative GLP-1 levels were significantly increased in PHLF(P=0.02).Furthermore,incorporation of GLP-1 into the established aspartate aminotransferase to platelet ratio index(APRI)+albumin-bilirubin(ALBI)score,improved PHLF prediction[area under the curve(AUC):0.833,95%confidence interval(CI):0.660-0.964].Conclusions:Significant metabolic changes occur during human liver resection,particularly in phospholipid metabolism,along with distinct perioperative dynamics of GLP-1 and GLP-2,closely linked to PHLF and independent of the histological phenotype of MASLD.Additionally,we provide exploratory results on the predictive value of GLP-1 for PHLF,emphasizing a holistic model of liver function assessment highlighting the metabolic component of human liver regeneration.展开更多
The relative xity of a permutation group is the maximum proportion of the points xed by a non-trivial element of the group,and the relative xity of a graph is the relative xity of its automorphism group,viewed as a pe...The relative xity of a permutation group is the maximum proportion of the points xed by a non-trivial element of the group,and the relative xity of a graph is the relative xity of its automorphism group,viewed as a permutation group on the vertex-set of the graph.We prove in this paper that the relative xity of connected 2-arc-transitive graphs of a xed valence tends to 0 as the number of vertices grows to in nity.We prove the same result for the class of arc-transitive graphs of a xed prime valence,and more generally,for any class of arc-transitive locally-L graphs,where L is a xed quasiprimitive graph-restrictive permutation group.展开更多
基金supported by the Austrian government“Bürgermeisterfonds,Vienna,Austria”(18129)NIH(R01DK122813)+2 种基金the Austrian Science Fund(FWF)(P-32064)“City of Vienna Fund for Innovative Interdisciplinary Cancer Research”(21041)supported by means of the IHPBA Kenneth Warren Fellowship during conducting this study.
文摘Background:Metabolic regulation is critical during liver regeneration in rodents,but human data are limited.We investigated perioperative dynamics of circulating metabolites and plasma levels of glucagon-like peptide-1(GLP-1)and GLP-2,in patients undergoing liver resections,exploring their associations with the histological phenotype of metabolic dysfunction-associated steatotic liver disease(MASLD)and posthepatectomy liver failure(PHLF).Methods:Eighty-one and 75 patients from two centers between 2012 and 2023 were studied.Targeted quantitative metabolomic assay of 180 circulating metabolites,perioperative GLP-1,GLP-2,and standard lipid parameter level evaluation was employed.An exploratory PHLF prediction model was developed,including GLP-1 as a metabolic parameter.Results:Significant alterations of 44 metabolites by postoperative day(POD)1 and 40 by POD5 were observed,mainly among phospholipid species.Unsupervised clustering identified two metabolic clusters,with one encompassing 93%of PHLF patients by POD5(P<0.001).Standard plasma lipid parameters displayed consistent decrease after hepatectomy,independent from MASLD phenotype,with the lowest levels in PHLF patients.Postoperative GLP-1 and GLP-2 dynamics displayed a reciprocal pattern,indicating adaptive change in secretion.Preoperative GLP-1 levels were significantly increased in PHLF(P=0.02).Furthermore,incorporation of GLP-1 into the established aspartate aminotransferase to platelet ratio index(APRI)+albumin-bilirubin(ALBI)score,improved PHLF prediction[area under the curve(AUC):0.833,95%confidence interval(CI):0.660-0.964].Conclusions:Significant metabolic changes occur during human liver resection,particularly in phospholipid metabolism,along with distinct perioperative dynamics of GLP-1 and GLP-2,closely linked to PHLF and independent of the histological phenotype of MASLD.Additionally,we provide exploratory results on the predictive value of GLP-1 for PHLF,emphasizing a holistic model of liver function assessment highlighting the metabolic component of human liver regeneration.
基金supported by the Austrian Science Fund(FWF)Project W1230-N13.The second author was supported by the Research Programme P1-0294the Research Project J1-1691,both funded by the Slovenian Research Agency(ARRS).
文摘The relative xity of a permutation group is the maximum proportion of the points xed by a non-trivial element of the group,and the relative xity of a graph is the relative xity of its automorphism group,viewed as a permutation group on the vertex-set of the graph.We prove in this paper that the relative xity of connected 2-arc-transitive graphs of a xed valence tends to 0 as the number of vertices grows to in nity.We prove the same result for the class of arc-transitive graphs of a xed prime valence,and more generally,for any class of arc-transitive locally-L graphs,where L is a xed quasiprimitive graph-restrictive permutation group.
