Objective:To report the renal histological lesions in synanthropic rodents,Mus musculus and Rattus rattus,naturally infected with Leptospira spp.,captured in a rural community in Yucatan,Mexico.Methods:Kidney samples ...Objective:To report the renal histological lesions in synanthropic rodents,Mus musculus and Rattus rattus,naturally infected with Leptospira spp.,captured in a rural community in Yucatan,Mexico.Methods:Kidney samples of synanthropic rodents were collected from a rural community in Yucatan,Mexico.Polymerase chain reaction was used to detect Leptospira spp.infection.Tissue kidney was fixed in 10%buffered formalin,processed according to the usual techniques for paraffin inclusion,cut and stained with hematoxylin and eosin,and examined using a conventional electronic microscope.Results:A total of 187 rodents were captured.Nine individuals(4.8%)were positive for Leptospira spp.in the molecular analysis.All renal lesions observed in the histopathological study had been reported previously for Leptospira spp.infection.Conclusions:The histopathological lesions are present in the kidneys,plus the results of the polymerase chain reaction confirm that these rodents are true carriers of Leptospira spp.展开更多
基金supported by PROMEP-México-Proyecto 103.5/09/1258(Red Epidemiológica de Enfermedades Zoonóticas y Transmitidas por Vector de Importancia en Salud Pública)Consejo Nacional de Ciencia y Tecnología de México(No.grant320021)
文摘Objective:To report the renal histological lesions in synanthropic rodents,Mus musculus and Rattus rattus,naturally infected with Leptospira spp.,captured in a rural community in Yucatan,Mexico.Methods:Kidney samples of synanthropic rodents were collected from a rural community in Yucatan,Mexico.Polymerase chain reaction was used to detect Leptospira spp.infection.Tissue kidney was fixed in 10%buffered formalin,processed according to the usual techniques for paraffin inclusion,cut and stained with hematoxylin and eosin,and examined using a conventional electronic microscope.Results:A total of 187 rodents were captured.Nine individuals(4.8%)were positive for Leptospira spp.in the molecular analysis.All renal lesions observed in the histopathological study had been reported previously for Leptospira spp.infection.Conclusions:The histopathological lesions are present in the kidneys,plus the results of the polymerase chain reaction confirm that these rodents are true carriers of Leptospira spp.
