The mucilage fraction from Ora-pro-Nobis(OPN)leaves was extracted and characterized regarding its chemical composition,rheological behavior,and prebiotic potential.The extraction involved hot water treatment followed ...The mucilage fraction from Ora-pro-Nobis(OPN)leaves was extracted and characterized regarding its chemical composition,rheological behavior,and prebiotic potential.The extraction involved hot water treatment followed by ethanol precipitation,resulting in a substantial extraction yield(4.77%of total leaves).This mucilage extraction served as an initial step for subsequent protein extraction from residual leaf material.OPN mucilage primarily comprises a high molecular weight polysaccharide(arabinogalactan),which upon hydrolysis,yields xylose(30.11%w/w)and arabinose(29.15%w/w)as primary components,alongside minor amounts of glucose(5.28%w/w)and cellobiose(3.80%w/w).Rheological analysis indicated pseudoplastic behavior and thixotropic properties,offering potential value in formulating 3D printed food products.Furthermore,di-gestibility analysis demonstrated that the mucilage was highly resistant to simulated gastrointestinal conditions,with only 2.69%degradation observed.In addition,assays evaluating its prebiotic potential showed that the mucilage supported the growth of the probiotic strain Lactobacillus acidophilus.Furthermore.All these findings,combined with the classification of glucoarabinoxylan as a dietary or functional fiber,underscore the potential of OPN mucilage as an innovative ingredient for food product development,promoting microbiota balance.However,further research is warranted to fully explore the diverse applications of OPN mucilage as a structural and functional component in foods.展开更多
The meat analog market has significantly grown in recent years,increasing the demand for new ingredients.This study aimed to develop a vegetable meat analogue using soy protein isolate and Ora-pro-nobis(OPN)mucilage a...The meat analog market has significantly grown in recent years,increasing the demand for new ingredients.This study aimed to develop a vegetable meat analogue using soy protein isolate and Ora-pro-nobis(OPN)mucilage as a structuring agent,with application in 3D printing.Ora-pro-nobis mucilage,a complex polysaccharide with nutritional and technological potentials,was used as a structuring agent in 3D-printed soy protein-based meat analogs.Formulations with OPN mucilage(FMOPN),commercial hydrocolloids(FGG and FXG),and a formulation without hydrocolloids(FC)were compared.Rheological properties,texture,color,syneresis,microscopy,and printing accuracy were analyzed,and statistical comparisons between formulations were performed using univariate analysis with Tukey’s test.The FMOPN sample exhibited favorable rheological properties comparable to the FGG and FXG samples and superior to the control sample.All samples displayed pseudoplastic behavior consistent with the Ostwald-de Waele model,while FMOPN and FXG showed thixotropic behavior with viscosity recovery above 80%,which is essential for printing accuracy and structural stability.FMOPN achieved a maximum printing accuracy of 94.6%,outperforming FXG(93.34%),FGG(92.42%),and FC(85.7%).Additionally,the addition of mucilage reduced post-freeze/thaw syneresis to 22.5%(FMOPN),compared to 37.97%in the control sample,FC.However,mucilage influenced coloration,reducing lightness(L*)and redness(a*),due to the presence of chlorophyll,with additional darkening after cooking caused by pigment losses and the Maillard reaction.This study highlights the potential of OPN mucilage as an ingredient for plant-based meat analogue formulations,contributing to enhanced technological properties and stability in 3D printing.展开更多
基金supported by grants project from São Paulo Research Foundation(FAPESP)[project number:2022/016069]National Council for Scientific and Technological Development(CNPq-Brazil).
文摘The mucilage fraction from Ora-pro-Nobis(OPN)leaves was extracted and characterized regarding its chemical composition,rheological behavior,and prebiotic potential.The extraction involved hot water treatment followed by ethanol precipitation,resulting in a substantial extraction yield(4.77%of total leaves).This mucilage extraction served as an initial step for subsequent protein extraction from residual leaf material.OPN mucilage primarily comprises a high molecular weight polysaccharide(arabinogalactan),which upon hydrolysis,yields xylose(30.11%w/w)and arabinose(29.15%w/w)as primary components,alongside minor amounts of glucose(5.28%w/w)and cellobiose(3.80%w/w).Rheological analysis indicated pseudoplastic behavior and thixotropic properties,offering potential value in formulating 3D printed food products.Furthermore,di-gestibility analysis demonstrated that the mucilage was highly resistant to simulated gastrointestinal conditions,with only 2.69%degradation observed.In addition,assays evaluating its prebiotic potential showed that the mucilage supported the growth of the probiotic strain Lactobacillus acidophilus.Furthermore.All these findings,combined with the classification of glucoarabinoxylan as a dietary or functional fiber,underscore the potential of OPN mucilage as an innovative ingredient for food product development,promoting microbiota balance.However,further research is warranted to fully explore the diverse applications of OPN mucilage as a structural and functional component in foods.
基金The Sao Paulo Research Foundation(FAPESP)[project number:2022/016069]Coordination for the Improvement of Higher Education Personnel(CAPES-Brazil)National Council for Scientific and Technological Development(CNPq-Brazil).
文摘The meat analog market has significantly grown in recent years,increasing the demand for new ingredients.This study aimed to develop a vegetable meat analogue using soy protein isolate and Ora-pro-nobis(OPN)mucilage as a structuring agent,with application in 3D printing.Ora-pro-nobis mucilage,a complex polysaccharide with nutritional and technological potentials,was used as a structuring agent in 3D-printed soy protein-based meat analogs.Formulations with OPN mucilage(FMOPN),commercial hydrocolloids(FGG and FXG),and a formulation without hydrocolloids(FC)were compared.Rheological properties,texture,color,syneresis,microscopy,and printing accuracy were analyzed,and statistical comparisons between formulations were performed using univariate analysis with Tukey’s test.The FMOPN sample exhibited favorable rheological properties comparable to the FGG and FXG samples and superior to the control sample.All samples displayed pseudoplastic behavior consistent with the Ostwald-de Waele model,while FMOPN and FXG showed thixotropic behavior with viscosity recovery above 80%,which is essential for printing accuracy and structural stability.FMOPN achieved a maximum printing accuracy of 94.6%,outperforming FXG(93.34%),FGG(92.42%),and FC(85.7%).Additionally,the addition of mucilage reduced post-freeze/thaw syneresis to 22.5%(FMOPN),compared to 37.97%in the control sample,FC.However,mucilage influenced coloration,reducing lightness(L*)and redness(a*),due to the presence of chlorophyll,with additional darkening after cooking caused by pigment losses and the Maillard reaction.This study highlights the potential of OPN mucilage as an ingredient for plant-based meat analogue formulations,contributing to enhanced technological properties and stability in 3D printing.
