In neuroscience research,neuronal models are crucial tools for elucidating the molecular and cellular processes involved in the disorders of the nervous system.Facilitating easily and reproducibly executable studies,i...In neuroscience research,neuronal models are crucial tools for elucidating the molecular and cellular processes involved in the disorders of the nervous system.Facilitating easily and reproducibly executable studies,in vitro models,such as the SH-SY5Y cell line culture,help us explore the pathophysiological mechanisms of neurodegenerative diseases;they are also essential for the efficient screening of drugs for treating the diseases of the nervous system(Peng et al.,2021;Strother et al.,2021).展开更多
Cheese whey proteins are natural vehicles for biological agents that are used as an inexpensive and effective coating material in the encapsulation of probiotics.In this work,the yeast Kluyveromyces marxianus,of probi...Cheese whey proteins are natural vehicles for biological agents that are used as an inexpensive and effective coating material in the encapsulation of probiotics.In this work,the yeast Kluyveromyces marxianus,of probiotic potential,was associated with the prebiotic Plantago ovata(Psyllium)in particles coated with cheese whey.The efficiency of encapsulation,gastrointestinal tract(GIT)viability,particle morphology,and adhesion to enteral cells were analyzed.K.marxianus was encapsulated in different formulations with alginate(alg),psyllium(psy),and cheese whey(cw)through an extrusion process associated with vibrational technology.Yeasts encapsulated in different wall materials[alginate-psyllium-cheese whey(alg-psy-cw),alginate(alg),alginate-psyllium(alg-psy),and alginate-cheese whey(alg-cw)]were adequately protected,resulting in high cell viability(11.32,9.77,9.13,10.95 log CFU.g1,respectively).Regarding the bioavailability assay after three months of storage under refrigeration(±4℃),cells encapsulated in wall material alg-psy-cw demonstrated the best viability(>6 log CFU.g^(-1)).Encapsulated yeasts showed greater resistance to acidic conditions when exposed to simulations of the gastrointestinal tract,demonstrating a lower diffusion rate in the formulation made with wall material alg-psy-cw(10.03 log CFU.g^(-1)).The adhesion rate to Caco-2 cells was high(±3,166 yeasts/100 Caco-2 cells).Finally,the developed product in this study was found to be suitable for incorporation in functional foods,as well as in pharmaceutical products,demonstrating its biotechnological potential.展开更多
基金supported in part by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior(Capes-Brazil),No.001(to MIG).
文摘In neuroscience research,neuronal models are crucial tools for elucidating the molecular and cellular processes involved in the disorders of the nervous system.Facilitating easily and reproducibly executable studies,in vitro models,such as the SH-SY5Y cell line culture,help us explore the pathophysiological mechanisms of neurodegenerative diseases;they are also essential for the efficient screening of drugs for treating the diseases of the nervous system(Peng et al.,2021;Strother et al.,2021).
基金This work was supported by the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico(CNPq)(grant no.308515/2020-0)University of Vale do Taquari-Univates.
文摘Cheese whey proteins are natural vehicles for biological agents that are used as an inexpensive and effective coating material in the encapsulation of probiotics.In this work,the yeast Kluyveromyces marxianus,of probiotic potential,was associated with the prebiotic Plantago ovata(Psyllium)in particles coated with cheese whey.The efficiency of encapsulation,gastrointestinal tract(GIT)viability,particle morphology,and adhesion to enteral cells were analyzed.K.marxianus was encapsulated in different formulations with alginate(alg),psyllium(psy),and cheese whey(cw)through an extrusion process associated with vibrational technology.Yeasts encapsulated in different wall materials[alginate-psyllium-cheese whey(alg-psy-cw),alginate(alg),alginate-psyllium(alg-psy),and alginate-cheese whey(alg-cw)]were adequately protected,resulting in high cell viability(11.32,9.77,9.13,10.95 log CFU.g1,respectively).Regarding the bioavailability assay after three months of storage under refrigeration(±4℃),cells encapsulated in wall material alg-psy-cw demonstrated the best viability(>6 log CFU.g^(-1)).Encapsulated yeasts showed greater resistance to acidic conditions when exposed to simulations of the gastrointestinal tract,demonstrating a lower diffusion rate in the formulation made with wall material alg-psy-cw(10.03 log CFU.g^(-1)).The adhesion rate to Caco-2 cells was high(±3,166 yeasts/100 Caco-2 cells).Finally,the developed product in this study was found to be suitable for incorporation in functional foods,as well as in pharmaceutical products,demonstrating its biotechnological potential.
