BACKGROUND:Postpartum posttraumatic stress disorder(PTSD)can occur in women who give birth after emergency admission.The identification of risk factors for this condition is crucial for developing effective preventive...BACKGROUND:Postpartum posttraumatic stress disorder(PTSD)can occur in women who give birth after emergency admission.The identification of risk factors for this condition is crucial for developing effective preventive measures.This retrospective study aimed to explore the incidence and risk factors for postpartum PTSD in women who give birth after emergency admission.METHODS:Medical records of women who gave birth after emergency admission were collected between March 2021 and April 2023.The patients’general conditions and perinatal clinical indicators were recorded.The puerperae were divided into PTSD group and control group based on symptom occurrence at six weeks postpartum.Multivariate logistic regression analysis was performed to identify risk factors.RESULTS:A total of 276 puerperae were included,with a PTSD incidence of 20.3% at six weeks postpartum.Multivariate logistic regression analysis identified emergency cesarean section(odds ratio[OR]=2.102;95%confidence interval[CI]:1.114-3.966,P=0.022),admission to the emergency department after midnight(12:00 AM)(OR=2.245;95%CI:1.170-4.305,P<0.001),and cervical dilation(OR=3.203;95%CI:1.670–6.141,P=0.039)as independent risk factors for postpartum PTSD.Analgesia pump use(OR=0.500;95%CI:0.259–0.966,P=0.015)was found to be a protective factor against postpartum PTSD.CONCLUSION:Emergency cesarean section,admission to the emergency department after midnight,and cervical dilation were identified as independent risk factors for postpartum PTSD,while analgesic pump use was a protective factor.These findings provide insights for developing more effective preventive measures for women who give birth after emergency admission.展开更多
Histone methylation is a kind of important epigenetic modification which occurs on the lysine residue or arginine residue of histone tails（Zhang and Reinberg,2001）.It takes part in multiple biological processes,incl...Histone methylation is a kind of important epigenetic modification which occurs on the lysine residue or arginine residue of histone tails（Zhang and Reinberg,2001）.It takes part in multiple biological processes,including gene expression,genomic stability,stem cell maturity,genetic imprinting,mitosis and development（Fischle et al.,2005）.展开更多
Background:Induced pluripotent stem cells(iPSCs)and embryonic stem cells(ESCs)share many common features,including similar morphology,gene expression and in vitro differentiation profiles.However,genomic stability is ...Background:Induced pluripotent stem cells(iPSCs)and embryonic stem cells(ESCs)share many common features,including similar morphology,gene expression and in vitro differentiation profiles.However,genomic stability is much lower in iPSCs than in ESCs.In the current study,we examined whether changes in DNA damage repair in iPSCs are responsible for their greater tendency towards mutagenesis.Methods:Mouse iPSCs,ESCs and embryonic fibroblasts were exposed to ionizing radiation(4 Gy)to introduce dou-ble-strand DNA breaks.At 4 h later,fidelity of DNA damage repair was assessed using whole-genome re-sequencing.We also analyzed genomic stability in mice derived from iPSCs versus ESCs.Results:In comparison to ESCs and embryonic fibroblasts,iPSCs had lower DNA damage repair capacity,more somatic mutations and short indels after irradiation.iPSCs showed greater non-homologous end joining DNA repair and less homologous recombination DNA repair.Mice derived from iPSCs had lower DNA damage repair capacity than ESC-derived mice as well as C57 control mice.Conclusions:The relatively low genomic stability of iPSCs and their high rate of tumorigenesis in vivo appear to be due,at least in part,to low fidelity of DNA damage repair.展开更多
DNA methylation plays an essential role in mammalian development[1].However,how DNA methylation is inherited between generations and if there is family-specific DNA methylation pattern remains to be elucidated[2].In t...DNA methylation plays an essential role in mammalian development[1].However,how DNA methylation is inherited between generations and if there is family-specific DNA methylation pattern remains to be elucidated[2].In this study,we collect male blood samples including a big pedigree of the descendants of an ancient Chinese empire and samples from different haplogroups to study their whole genome DNA methylation pattern.We find 115 male family-specific methylation sites from three families.The difference of whole genome DNA methylation pattern correlates展开更多
基金Science and Technology Development Plan Project of Suzhou(SKJYD2021035)Science and Technology Development Plan Project of Suzhou(SKJYD2022078)The Key Project Research Fund of the Second Affiliated Hospital of Wannan Medical College(YK2023Z04)。
文摘BACKGROUND:Postpartum posttraumatic stress disorder(PTSD)can occur in women who give birth after emergency admission.The identification of risk factors for this condition is crucial for developing effective preventive measures.This retrospective study aimed to explore the incidence and risk factors for postpartum PTSD in women who give birth after emergency admission.METHODS:Medical records of women who gave birth after emergency admission were collected between March 2021 and April 2023.The patients’general conditions and perinatal clinical indicators were recorded.The puerperae were divided into PTSD group and control group based on symptom occurrence at six weeks postpartum.Multivariate logistic regression analysis was performed to identify risk factors.RESULTS:A total of 276 puerperae were included,with a PTSD incidence of 20.3% at six weeks postpartum.Multivariate logistic regression analysis identified emergency cesarean section(odds ratio[OR]=2.102;95%confidence interval[CI]:1.114-3.966,P=0.022),admission to the emergency department after midnight(12:00 AM)(OR=2.245;95%CI:1.170-4.305,P<0.001),and cervical dilation(OR=3.203;95%CI:1.670–6.141,P=0.039)as independent risk factors for postpartum PTSD.Analgesia pump use(OR=0.500;95%CI:0.259–0.966,P=0.015)was found to be a protective factor against postpartum PTSD.CONCLUSION:Emergency cesarean section,admission to the emergency department after midnight,and cervical dilation were identified as independent risk factors for postpartum PTSD,while analgesic pump use was a protective factor.These findings provide insights for developing more effective preventive measures for women who give birth after emergency admission.
基金supported by the National Natural Science Foundation of China(Nos.31540033 and91131002)the Precision Medicine Research Program of the Chinese Academy of Sciences(KJZD-EW-L14)+2 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA12020343)the National Basic Research Program of China(2013CB911001 and 2012CB518302)the National Excellent Youth Science Foundation of China(No.31222030)
文摘Histone methylation is a kind of important epigenetic modification which occurs on the lysine residue or arginine residue of histone tails（Zhang and Reinberg,2001）.It takes part in multiple biological processes,including gene expression,genomic stability,stem cell maturity,genetic imprinting,mitosis and development（Fischle et al.,2005）.
基金supported by the Precision Medicine Research Program of the Chinese Academy of Sciences(KJZD-EW-L14)Strategic Priority Research Program of the Chinese Academy of Sciences(XDA01040407)+2 种基金National Natural Science Foundation of China(31471395,91019024,31540033 and 31100558)National Basic Research Program of China(973 Program,2012CB518302 and 2013CB911001)100 Talents Project.
文摘Background:Induced pluripotent stem cells(iPSCs)and embryonic stem cells(ESCs)share many common features,including similar morphology,gene expression and in vitro differentiation profiles.However,genomic stability is much lower in iPSCs than in ESCs.In the current study,we examined whether changes in DNA damage repair in iPSCs are responsible for their greater tendency towards mutagenesis.Methods:Mouse iPSCs,ESCs and embryonic fibroblasts were exposed to ionizing radiation(4 Gy)to introduce dou-ble-strand DNA breaks.At 4 h later,fidelity of DNA damage repair was assessed using whole-genome re-sequencing.We also analyzed genomic stability in mice derived from iPSCs versus ESCs.Results:In comparison to ESCs and embryonic fibroblasts,iPSCs had lower DNA damage repair capacity,more somatic mutations and short indels after irradiation.iPSCs showed greater non-homologous end joining DNA repair and less homologous recombination DNA repair.Mice derived from iPSCs had lower DNA damage repair capacity than ESC-derived mice as well as C57 control mice.Conclusions:The relatively low genomic stability of iPSCs and their high rate of tumorigenesis in vivo appear to be due,at least in part,to low fidelity of DNA damage repair.
基金supported by Precision Medicine Research Program of the Chinese Academy of Sciences(KJZD-EWL14)Strategic Priority Research Program of the Chinese Academy of Sciences(XDA01040407)+2 种基金National Natural Science Foundation of China(31471395,91019024,31540033 and 31100558)National Basic Research Program of China(2012CB518302 and2013CB911001)One Hundred Person Project of the Chinese Academy of Sciences
文摘DNA methylation plays an essential role in mammalian development[1].However,how DNA methylation is inherited between generations and if there is family-specific DNA methylation pattern remains to be elucidated[2].In this study,we collect male blood samples including a big pedigree of the descendants of an ancient Chinese empire and samples from different haplogroups to study their whole genome DNA methylation pattern.We find 115 male family-specific methylation sites from three families.The difference of whole genome DNA methylation pattern correlates
