Class Ⅲ peroxidases(CⅢ Prxs) play critical roles in plant immunity by scavenging reactive oxygen species(ROS). However, the functions of CⅢ Prxs in rice(Oryza sativa L.) immunity are largely unexplored. Here, we re...Class Ⅲ peroxidases(CⅢ Prxs) play critical roles in plant immunity by scavenging reactive oxygen species(ROS). However, the functions of CⅢ Prxs in rice(Oryza sativa L.) immunity are largely unexplored. Here, we report a Prx precursor, OsPrx30,that is responsive to the bacterial blight Xanthomonas oryzae pv. oryzae(Xoo). OsPrx30 was primarily expressed in rice roots, leaves, and stems,and its protein product was mainly localized at the endoplasmic reticulum. Overexpression of OsPrx30 enhanced the plant’s susceptibility to Xoo by maintaining a high level of peroxidase(POD) activity and reducing the content of H2O2, whereas depletion of OsPrx30 had the opposite effects. Furthermore, we identified an AT-hook transcription factor, Os ATH1, that is specifically bound to the OsPrx30 promoter. As observed in plants overexpressing OsPrx30, depletion of Os ATH1 enhanced susceptibility to Xoo. Finally, we demonstrated that depletion of Os ATH1 increased histone H3 acetylation at the AT-rich region of the OsPrx30 promoter.Taken together, these results reveal a mechanism underlying the POD-induced natural resistance to bacterial diseases and suggest a model for transcription regulation of Prx genes in rice.展开更多
With the objective of obtaining a completely straw-mulched field,when no-tillage mechanical sowing is implemented with straw smashing,delivering,and back-throwing approaches,it may be difficult to scatter the smashed ...With the objective of obtaining a completely straw-mulched field,when no-tillage mechanical sowing is implemented with straw smashing,delivering,and back-throwing approaches,it may be difficult to scatter the smashed straw uniformly during a succeeding wheat sowing step.This is because the previous rice straw is substantial in quantity and has a high humidity and toughness,which may easily result in non-uniform straw mulching and thus sparse and weak seedlings of wheat.Therefore a force-dispersing and uniform-scattering device was designed.With the number of scattering impellers,impeller angle,and impeller rotation speed as the main factors and the percentage of pass for the scattering width and non-uniformity of the straw mulching as the assessment indices,single-factor experiments and orthogonal regressive tests were performed,and a dual-index(percentage of pass for the scattering width and non-uniformity of the straw mulching)fitted regression equation was established.The test results suggested that the main factors(from primary to secondary)that influence the indices were the impeller rotation speed,number of scattering impellers,and impeller angle.The optimal parameter combination for the uniform scattering device was four rows of impellers with an angle of 15°,rotation speed of 1015 r/min,percentage of pass of 72.65%for the scattering width,and a non-uniformity of 13.8%in the straw mulching.This combination can be used to realize a uniform scattering of the smashed straw along the seedling rows on the after-sowing ground.According to the field investigation of the wheat growth,the wheat emergence rate was 90.7%.The research results can provide a reference for improving the uniform scattering device for a straw-smashing,back-throwing,no-tillage planter for obtaining a completely straw-mulched field,enhancing the quality of the machinery operation,and ensuring good and strong seedlings after sowing.展开更多
Restitution coefficient(RC)of garlic bulb is an important mechanical property that is required to establish the kinematics model of bulb collision and research the damage mechanism of bulb collision.In this study,kine...Restitution coefficient(RC)of garlic bulb is an important mechanical property that is required to establish the kinematics model of bulb collision and research the damage mechanism of bulb collision.In this study,kinetic equations of bulb collision were established based on Hertz's contact theory.The kinematics characteristics,elastoplastic deformation and contact damage during bulb collision were analyzed by using high-speed photography.The effects of bulb mass,moisture content,collision material,material thickness and release height on the RC were investigated by mixed orthogonal experiments and single-factor experiments.The results showed that the movement of bulb in the compression stage was translation,and the movement in the rebound stage was translation and rotation.During collision,the larger the rotational angular velocity of the bulb was,the smaller the measured RC would be.The contact damage of bulb included internal damage of the tissue,epidermis stretch and tear.The significance of effects of factors on RC decreased with the following sequence:collision material,release height,material thickness,bulb mass,and moisture content.Collision material,release height,material thickness,and bulb mass were significant factors.The RC between the bulb and Q235,nylon,and rubber decreased sequentially.The RC decreased with the increase of release height and bulb mass.The RC increased with the increase of material thickness of Q235,while it decreased with the increase of material thickness of rubber or Nylon.The determination coefficients of the regression equations between the significant factors and the RC were all greater than 0.96.The results will be helpful for damage mechanism analysis and design of garlic production equipment.展开更多
Research interest in pneumatic conveying technologies in processes such as peanut harvesting and shelling has grown rapidly in recent years.However,the use of pneumatic conveyors in this application suffers from high ...Research interest in pneumatic conveying technologies in processes such as peanut harvesting and shelling has grown rapidly in recent years.However,the use of pneumatic conveyors in this application suffers from high pod damage rates and duct obstruction.To address these issues,we analyzed the critical speed of pneumatic transport for conveying the peanut pods and measured the angle of friction and coefficient of restitution of peanut pods on a variety of material surfaces.Based on the results of these tests,optimizations and improvements were made to the separator bowl,air supply duct,and conveying duct.A pneumatic conveying experiment was then performed using peanut pods.In the factorial experiment,it was found that increases in fan speed increase the pod damage rate and transport efficiency,while increases in the thickness of the cushioning/anti-obstruction layer decrease the rate of pod damage and transport efficiency.Pod damage rates were significantly affected by fan speed,the thickness of the cushioning/obstruction prevention layer,and interaction between these factors,while transport efficiency was only significantly affected by fan speed.It is proved by the machine verification test,the optimal parameters for the pneumatic transport of Baisha peanut pods with a moisture content of 7.24%was a fan speed of 2700 r/min and a cushioning/anti-obstruction layer thickness of 6 mm.A pod damage rate of 5.19%and transport efficiency of 92.03%were achieved using these parameters,which are sufficient for meeting the requirements of industrial applications.展开更多
Accelerate the quality of smashed-straw laying and enhance the effect of seed-bed arranging for no-tillage planter with straw-smashing and strip-mulching in full stubble covered paddy have become imperative in impleme...Accelerate the quality of smashed-straw laying and enhance the effect of seed-bed arranging for no-tillage planter with straw-smashing and strip-mulching in full stubble covered paddy have become imperative in implementing modern conservation tillage.Considering the perfect operating performance(passability and stability)of the developed no-tillageplanter,this study intends to optimize the structure design of smashed-straw diversion device and strip-rotary tillage device.Dynamics equations of smashed straw and kinematics models of rotary blades were established through theoretical analysis,and the principal factors that affecting straw strip-laying quality and seed-bed arranging effect were specified.The influence of out-enlarge angle(η)and slide-push angle(γ)of the diversion device on the coefficient of variation(ζ1)of cover-straw width,and the influence of rotary tillage-blade number(N)and configuration in a singlerotary plane on the broken rate(ζ2)of strip soil were completely analyzed.And then,based on the systematic analysis and integrated scheme,operating performance and field verification tests using the optimized no-tillage planter were thoroughly performed.The results of the performance tests indicated that the out-enlarge angle(η)had a highly significant influence on the coefficient of variation(ζ1),and the slide-push angle(γ)had a significant influence on(ζ1).The rotary tillage-blade number(N)had a highly significant influence on the broken rate(ζ2),and the slide-push angle(γ)had a significant influence on(ζ2).The obtained optimal combination of these key structure parameters through comprehensive analysis wasη=45°,γ=40°,and N=4.Field verification test results indicated that the optimized no-tillage planter achieved mean values of ζ1=10.47%and ζ2=90.95%,which satisfied the relevant operation quality and cultivation agricultural requirement of conservation tillage equipment,and provided technical references for developing the similar no-tillage planter of straw crushing and returning.展开更多
Uniform crushed straw throwing and seed-sowing machines can achieve the processes of straw chopping,straw transport,sowing,fertilization,and straw mulching at the same time,which is widely used in many areas of China....Uniform crushed straw throwing and seed-sowing machines can achieve the processes of straw chopping,straw transport,sowing,fertilization,and straw mulching at the same time,which is widely used in many areas of China.Conveying device is one of the important components used to convey,elevate and throw straw.However,the problems of high power consumption and congestion affect the promotion of the machine.Therefore,the conveying device of uniform crushed straw throwing and seed-sowing machine was analyzed in order to determine its device operation mechanism.Kinematic and dynamic analyses of particles of crushed rice straw during lifting and dispersion are used to develop a flexible-body model of rod-shaped and agglomerate-shaped crushed straw and a coupling model including the mechanical structure of the device.By integrating computational fluid dynamics and the discrete element method,the gas-solid coupling theory in numerical simulations and motion analysis of crushed straw particles is used to determine how the flow field and motion characteristics affect the conveying performance.Besides,regression equations to describe the relationships between the factors and each assessment index were established by using the regression analysis and response surface analysis with the software Design-Expert.The effect of throwing blade speed X_(1),conveying volume of crushed straw X_(2),and pipeline diameter X_(3) on the throwing speed of crushed straw Y_(1) and specific power consumption Y2 were investigated.The highest throwing speed of crushed straw and lowest specific power consumption are the optimization goal.The results of optimization showed that the predict the best optimal parameters were 2000 r/min throwing blade rotational speed,1.4 kg/s conveying volume,and 220 mm pipeline diameter,the planter achieved a throwing speed of 12.2 m/s and specific power consumption of 9179 m^(2)/s^(2).And then a field test verification was conducted.The planter achieved a throwing speed 12.4 m/s and specific power consumption 9070 m^(2)/s^(2) while selecting the best optimal parameters.Thus,the optimal parameters can provide a high-performance operation and satisfy the actual operation requirements The results provide a theoretical basis and data support for seeding technology innovation and equipment optimization to ensure uniform crushed straw throwing in dense rice stubble fields.展开更多
基金supported by the Natural Science Foundation of Guangdong Province of China (2018A0303130340)the Guangdong Basic and Applied Basic Research Foundation (2020A1515010021)+1 种基金the Research and Development Program in key areas of Guangdong Province (2018B020206002)the National Natural Science Foundation of China (31401722)。
文摘Class Ⅲ peroxidases(CⅢ Prxs) play critical roles in plant immunity by scavenging reactive oxygen species(ROS). However, the functions of CⅢ Prxs in rice(Oryza sativa L.) immunity are largely unexplored. Here, we report a Prx precursor, OsPrx30,that is responsive to the bacterial blight Xanthomonas oryzae pv. oryzae(Xoo). OsPrx30 was primarily expressed in rice roots, leaves, and stems,and its protein product was mainly localized at the endoplasmic reticulum. Overexpression of OsPrx30 enhanced the plant’s susceptibility to Xoo by maintaining a high level of peroxidase(POD) activity and reducing the content of H2O2, whereas depletion of OsPrx30 had the opposite effects. Furthermore, we identified an AT-hook transcription factor, Os ATH1, that is specifically bound to the OsPrx30 promoter. As observed in plants overexpressing OsPrx30, depletion of Os ATH1 enhanced susceptibility to Xoo. Finally, we demonstrated that depletion of Os ATH1 increased histone H3 acetylation at the AT-rich region of the OsPrx30 promoter.Taken together, these results reveal a mechanism underlying the POD-induced natural resistance to bacterial diseases and suggest a model for transcription regulation of Prx genes in rice.
基金This research work is a part of the Special funding project of National Modern Agricultural Peanut Industrial Technology System Construction(CARS-14-mechanical equipment)buried fruit harvester of the innovation project of Chinese Academy of Agricultural Sciences.
文摘With the objective of obtaining a completely straw-mulched field,when no-tillage mechanical sowing is implemented with straw smashing,delivering,and back-throwing approaches,it may be difficult to scatter the smashed straw uniformly during a succeeding wheat sowing step.This is because the previous rice straw is substantial in quantity and has a high humidity and toughness,which may easily result in non-uniform straw mulching and thus sparse and weak seedlings of wheat.Therefore a force-dispersing and uniform-scattering device was designed.With the number of scattering impellers,impeller angle,and impeller rotation speed as the main factors and the percentage of pass for the scattering width and non-uniformity of the straw mulching as the assessment indices,single-factor experiments and orthogonal regressive tests were performed,and a dual-index(percentage of pass for the scattering width and non-uniformity of the straw mulching)fitted regression equation was established.The test results suggested that the main factors(from primary to secondary)that influence the indices were the impeller rotation speed,number of scattering impellers,and impeller angle.The optimal parameter combination for the uniform scattering device was four rows of impellers with an angle of 15°,rotation speed of 1015 r/min,percentage of pass of 72.65%for the scattering width,and a non-uniformity of 13.8%in the straw mulching.This combination can be used to realize a uniform scattering of the smashed straw along the seedling rows on the after-sowing ground.According to the field investigation of the wheat growth,the wheat emergence rate was 90.7%.The research results can provide a reference for improving the uniform scattering device for a straw-smashing,back-throwing,no-tillage planter for obtaining a completely straw-mulched field,enhancing the quality of the machinery operation,and ensuring good and strong seedlings after sowing.
基金This work was supported by the National Natural Science Foundation of China(Grant No.51805282)Funds for Central Universities,China(XDJK2018AC001)National Key R&D Program of China(2017YFD0701305-02).
文摘Restitution coefficient(RC)of garlic bulb is an important mechanical property that is required to establish the kinematics model of bulb collision and research the damage mechanism of bulb collision.In this study,kinetic equations of bulb collision were established based on Hertz's contact theory.The kinematics characteristics,elastoplastic deformation and contact damage during bulb collision were analyzed by using high-speed photography.The effects of bulb mass,moisture content,collision material,material thickness and release height on the RC were investigated by mixed orthogonal experiments and single-factor experiments.The results showed that the movement of bulb in the compression stage was translation,and the movement in the rebound stage was translation and rotation.During collision,the larger the rotational angular velocity of the bulb was,the smaller the measured RC would be.The contact damage of bulb included internal damage of the tissue,epidermis stretch and tear.The significance of effects of factors on RC decreased with the following sequence:collision material,release height,material thickness,bulb mass,and moisture content.Collision material,release height,material thickness,and bulb mass were significant factors.The RC between the bulb and Q235,nylon,and rubber decreased sequentially.The RC decreased with the increase of release height and bulb mass.The RC increased with the increase of material thickness of Q235,while it decreased with the increase of material thickness of rubber or Nylon.The determination coefficients of the regression equations between the significant factors and the RC were all greater than 0.96.The results will be helpful for damage mechanism analysis and design of garlic production equipment.
文摘Research interest in pneumatic conveying technologies in processes such as peanut harvesting and shelling has grown rapidly in recent years.However,the use of pneumatic conveyors in this application suffers from high pod damage rates and duct obstruction.To address these issues,we analyzed the critical speed of pneumatic transport for conveying the peanut pods and measured the angle of friction and coefficient of restitution of peanut pods on a variety of material surfaces.Based on the results of these tests,optimizations and improvements were made to the separator bowl,air supply duct,and conveying duct.A pneumatic conveying experiment was then performed using peanut pods.In the factorial experiment,it was found that increases in fan speed increase the pod damage rate and transport efficiency,while increases in the thickness of the cushioning/anti-obstruction layer decrease the rate of pod damage and transport efficiency.Pod damage rates were significantly affected by fan speed,the thickness of the cushioning/obstruction prevention layer,and interaction between these factors,while transport efficiency was only significantly affected by fan speed.It is proved by the machine verification test,the optimal parameters for the pneumatic transport of Baisha peanut pods with a moisture content of 7.24%was a fan speed of 2700 r/min and a cushioning/anti-obstruction layer thickness of 6 mm.A pod damage rate of 5.19%and transport efficiency of 92.03%were achieved using these parameters,which are sufficient for meeting the requirements of industrial applications.
基金The authors acknowledge the financial support provided by the National Natural Science Foundation of China(Grant No.51905281)Jiangsu Agriculture Science and Technology Innovation Fund(Grant No.CX(17)1002)
文摘Accelerate the quality of smashed-straw laying and enhance the effect of seed-bed arranging for no-tillage planter with straw-smashing and strip-mulching in full stubble covered paddy have become imperative in implementing modern conservation tillage.Considering the perfect operating performance(passability and stability)of the developed no-tillageplanter,this study intends to optimize the structure design of smashed-straw diversion device and strip-rotary tillage device.Dynamics equations of smashed straw and kinematics models of rotary blades were established through theoretical analysis,and the principal factors that affecting straw strip-laying quality and seed-bed arranging effect were specified.The influence of out-enlarge angle(η)and slide-push angle(γ)of the diversion device on the coefficient of variation(ζ1)of cover-straw width,and the influence of rotary tillage-blade number(N)and configuration in a singlerotary plane on the broken rate(ζ2)of strip soil were completely analyzed.And then,based on the systematic analysis and integrated scheme,operating performance and field verification tests using the optimized no-tillage planter were thoroughly performed.The results of the performance tests indicated that the out-enlarge angle(η)had a highly significant influence on the coefficient of variation(ζ1),and the slide-push angle(γ)had a significant influence on(ζ1).The rotary tillage-blade number(N)had a highly significant influence on the broken rate(ζ2),and the slide-push angle(γ)had a significant influence on(ζ2).The obtained optimal combination of these key structure parameters through comprehensive analysis wasη=45°,γ=40°,and N=4.Field verification test results indicated that the optimized no-tillage planter achieved mean values of ζ1=10.47%and ζ2=90.95%,which satisfied the relevant operation quality and cultivation agricultural requirement of conservation tillage equipment,and provided technical references for developing the similar no-tillage planter of straw crushing and returning.
基金supported by the earmarked fund for CARS-13Natural Science Foundation of Jiangsu Province (Grant No.BK20221187).
文摘Uniform crushed straw throwing and seed-sowing machines can achieve the processes of straw chopping,straw transport,sowing,fertilization,and straw mulching at the same time,which is widely used in many areas of China.Conveying device is one of the important components used to convey,elevate and throw straw.However,the problems of high power consumption and congestion affect the promotion of the machine.Therefore,the conveying device of uniform crushed straw throwing and seed-sowing machine was analyzed in order to determine its device operation mechanism.Kinematic and dynamic analyses of particles of crushed rice straw during lifting and dispersion are used to develop a flexible-body model of rod-shaped and agglomerate-shaped crushed straw and a coupling model including the mechanical structure of the device.By integrating computational fluid dynamics and the discrete element method,the gas-solid coupling theory in numerical simulations and motion analysis of crushed straw particles is used to determine how the flow field and motion characteristics affect the conveying performance.Besides,regression equations to describe the relationships between the factors and each assessment index were established by using the regression analysis and response surface analysis with the software Design-Expert.The effect of throwing blade speed X_(1),conveying volume of crushed straw X_(2),and pipeline diameter X_(3) on the throwing speed of crushed straw Y_(1) and specific power consumption Y2 were investigated.The highest throwing speed of crushed straw and lowest specific power consumption are the optimization goal.The results of optimization showed that the predict the best optimal parameters were 2000 r/min throwing blade rotational speed,1.4 kg/s conveying volume,and 220 mm pipeline diameter,the planter achieved a throwing speed of 12.2 m/s and specific power consumption of 9179 m^(2)/s^(2).And then a field test verification was conducted.The planter achieved a throwing speed 12.4 m/s and specific power consumption 9070 m^(2)/s^(2) while selecting the best optimal parameters.Thus,the optimal parameters can provide a high-performance operation and satisfy the actual operation requirements The results provide a theoretical basis and data support for seeding technology innovation and equipment optimization to ensure uniform crushed straw throwing in dense rice stubble fields.
