To explore the optimum temperature,light intensityand water conditions for seed germination of Siphonostegia chinensis Benth.,seed germination experiment were carried out under different temperatures(5/15,10/20,15/25,...To explore the optimum temperature,light intensityand water conditions for seed germination of Siphonostegia chinensis Benth.,seed germination experiment were carried out under different temperatures(5/15,10/20,15/25,20/30℃),different light intensity(14h light/10h darkness,complete darkness)and different concentrations(0%,5%,10%,15%,20%)of PEG-6000 solution.In terms of concentration,5%PEG was regarded as the low level,10%and 15%as the medium level,and 20%as the high level.The results showed that(1)Germination rate,germination potential,and germination index were increased with the rise of temperature.In addition,seed germination was significantly higher under the dark conditions than that with the 14h light/10h darkness.(2)No seed germination occurred when the temperature was below 10/20 C at 14h light/10h darkness.(3)Under 14h light/10h darkness,the germination rate,germination potential and germination index first increased and then decreased with the increase of PEG concentration.The low concentration was more beneficial to the seed germination.(4)Under the condition of complete darkness,the germination rate,germination potential and germination index decline with fluctuation with the increase of PEG concentration.Seed germination of Siphonostegia chinensis Benth.was inhibited in high concentration of PEG.展开更多
With polyethylene glycol(PEG-6000),of 0%(CK),5%,10%,15%,25%used to simulate drought stress,and CaCl2 concentration 0(CK),of 15,20,25 and 30mmol/L as ion gradient of exogenous calcium,the effects of drought,exogenous c...With polyethylene glycol(PEG-6000),of 0%(CK),5%,10%,15%,25%used to simulate drought stress,and CaCl2 concentration 0(CK),of 15,20,25 and 30mmol/L as ion gradient of exogenous calcium,the effects of drought,exogenous calcium and the interaction between the two on the Datura seed germination,so as to explore the optimal application amount of exogenous calcium to ease the suppression of drought stress on Datura seed germination.The results showed that the germination rate,germination potential and germination index of the Datura seeds were significantly lower than those of the control group.Under the normal moisture condition,exogenous calcium of moderate and low concentration had no significant effect on the Datura seed germination,while that of high concentration showed an inhibitory effect on the seed germination.Under drought stress,with the increasing concentration of exogenous calcium,the three indicators of Datura seeds showed a trend of increasing first and then decreasing.When the exogenous calcium had the concentration of 20 mmol/L,all the indicators of seed germination reached the maximum value,while showed a downward trend when exogenous calcium concentration was 25-30 mmol/L,and even increasingly sharp with drought intensifying.Therefore,in the production and utilization of Datura,20 mmol/L of exogenous calcium can be used to soak seeds before sowing to improve the emergence rate under low and moderate drought conditions.展开更多
基金supported by the National Natural Science Foundation of China(NSFC:41801027)the National Natural Science Foundation of China(NSFC:3170043).
文摘To explore the optimum temperature,light intensityand water conditions for seed germination of Siphonostegia chinensis Benth.,seed germination experiment were carried out under different temperatures(5/15,10/20,15/25,20/30℃),different light intensity(14h light/10h darkness,complete darkness)and different concentrations(0%,5%,10%,15%,20%)of PEG-6000 solution.In terms of concentration,5%PEG was regarded as the low level,10%and 15%as the medium level,and 20%as the high level.The results showed that(1)Germination rate,germination potential,and germination index were increased with the rise of temperature.In addition,seed germination was significantly higher under the dark conditions than that with the 14h light/10h darkness.(2)No seed germination occurred when the temperature was below 10/20 C at 14h light/10h darkness.(3)Under 14h light/10h darkness,the germination rate,germination potential and germination index first increased and then decreased with the increase of PEG concentration.The low concentration was more beneficial to the seed germination.(4)Under the condition of complete darkness,the germination rate,germination potential and germination index decline with fluctuation with the increase of PEG concentration.Seed germination of Siphonostegia chinensis Benth.was inhibited in high concentration of PEG.
基金supported by the National Natural Science Foundation of China(NSFC:41801027)the National Natural Science Foundation of China(NSFC:31700434).
文摘With polyethylene glycol(PEG-6000),of 0%(CK),5%,10%,15%,25%used to simulate drought stress,and CaCl2 concentration 0(CK),of 15,20,25 and 30mmol/L as ion gradient of exogenous calcium,the effects of drought,exogenous calcium and the interaction between the two on the Datura seed germination,so as to explore the optimal application amount of exogenous calcium to ease the suppression of drought stress on Datura seed germination.The results showed that the germination rate,germination potential and germination index of the Datura seeds were significantly lower than those of the control group.Under the normal moisture condition,exogenous calcium of moderate and low concentration had no significant effect on the Datura seed germination,while that of high concentration showed an inhibitory effect on the seed germination.Under drought stress,with the increasing concentration of exogenous calcium,the three indicators of Datura seeds showed a trend of increasing first and then decreasing.When the exogenous calcium had the concentration of 20 mmol/L,all the indicators of seed germination reached the maximum value,while showed a downward trend when exogenous calcium concentration was 25-30 mmol/L,and even increasingly sharp with drought intensifying.Therefore,in the production and utilization of Datura,20 mmol/L of exogenous calcium can be used to soak seeds before sowing to improve the emergence rate under low and moderate drought conditions.
