In this study,we prepared paclitaxel/chitosan(PTX/CS)nanosuspensions(NSs)with different mass ratios of PTX and CS(1.5:2,2:2,and 2.5:2),for controlled drug delivery purposes.For attachment and dispersion in water mediu...In this study,we prepared paclitaxel/chitosan(PTX/CS)nanosuspensions(NSs)with different mass ratios of PTX and CS(1.5:2,2:2,and 2.5:2),for controlled drug delivery purposes.For attachment and dispersion in water medium,a simple ultrasonic disruption technique was employed.The water-dispersed PTX/CS NSs exhibited a rod-shape morphology with an average diameter of 170-210 nm and average length of about 1-10μm.Transmission electron microscopy,differential scan-ning calorimetry and X-ray diffraction indicated that the obtained PTX/CS NSs contain a nanocrystalline PTX phase.It was also inferred that presence of CS can promotes the crystalline nature of PTX up to 80%.In addition,efficiency of PTX loading reached over 85%in freeze-dried PTX/CS NSs,showing a slow rate of drug release in vitro for 8 days.The MTT and LDH assessments revealed that PTX/CS NSs significantly inhibit the growth of tumor cells(HeLa),while it is slightly toxic for the normal cells(NIH/3T3).Therefore,PTX/CS NSs is suggested as a potential nanodrug delivery system for cancer therapy.展开更多
基金National Natural Science Foundation of China(Grant No:51373099)State Key Laboratory of open funds of China from Donghua University(LK1411).
文摘In this study,we prepared paclitaxel/chitosan(PTX/CS)nanosuspensions(NSs)with different mass ratios of PTX and CS(1.5:2,2:2,and 2.5:2),for controlled drug delivery purposes.For attachment and dispersion in water medium,a simple ultrasonic disruption technique was employed.The water-dispersed PTX/CS NSs exhibited a rod-shape morphology with an average diameter of 170-210 nm and average length of about 1-10μm.Transmission electron microscopy,differential scan-ning calorimetry and X-ray diffraction indicated that the obtained PTX/CS NSs contain a nanocrystalline PTX phase.It was also inferred that presence of CS can promotes the crystalline nature of PTX up to 80%.In addition,efficiency of PTX loading reached over 85%in freeze-dried PTX/CS NSs,showing a slow rate of drug release in vitro for 8 days.The MTT and LDH assessments revealed that PTX/CS NSs significantly inhibit the growth of tumor cells(HeLa),while it is slightly toxic for the normal cells(NIH/3T3).Therefore,PTX/CS NSs is suggested as a potential nanodrug delivery system for cancer therapy.
